Study on the role of CNP in the regulation of oocyte meiosis

• Project/Area Number: 22580332
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Basic veterinary science/Basic zootechnical science
• Research Institution: Okayama University
• Principal Investigator: TSUJI Takehito 岡山大学, 大学院・環境生命科学研究科, 准教授 (90314682)
• Co-Investigator(Kenkyū-buntansha): KUNIEDA Tetsuo 岡山大学, 大学院・環境生命科学研究科, 教授 (80178011)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 卵母細胞 / 減数分裂 / CNP / LH

Research Abstract

This study investigated the role of CNP/NPR2 signaling in the regulation of oocyte meiosis, and obtained novel findings as follows: (1) CNP and its receptor (NPR2) were expressed in ovarian granulosa cells (2) mice with a mutation in Npr2 gene showed precocious resumption of oocyte meiosis in antral follicles (3) CNP was downregulated in response to LH signaling. These results suggest that the arrest of oocyte meiosis is maintained by CNP/NPR2 signaling from the granulosa cells that is relieved by LH signaling.

Research Products

• [Journal Article] CNP/NPR2 signaling maintains oocyte meiotic arrest in early antral follicles and is suppressed by EGFR-mediated signaling in preovulatory follicles (2012)
  • Author(s): Tsuji T, Kiyosu C, Akiyama K,KuniedaT
  • Journal Title: Mol Reprod Dev. Volume: 79巻 Issue: 11 Pages: 795-802
  • DOI: 10.1002/mrd.22114
  • Peer Reviewed
• [Journal Article] NPPC/NPR2 signaling is essential for oocyte meiotic arrest and cumulus oophorus formation during follicular development in the mouse ovary (2012)
  • Author(s): Kiyosu C, Tsuji T, Yamada K, Kajita S, Kunieda T
  • Journal Title: Reproduction Volume: 144巻 Issue: 2 Pages: 187-193
  • DOI: 10.1530/rep-12-0050
  • Peer Reviewed
• [Journal Article] A missense mutation of the Dhh gene is associated with male pseudohermaphroditic rats showing impaired Leydig cell development (2011)
  • Author(s): Kawai Y., その他
  • Journal Title: Reproduction Volume: 141 Pages: 217-225
  • Peer Reviewed
• [Journal Article] Gastrointestinal Tract Disorder in Natriuretic Peptide Receptor B Gene Mutant Mice (2010)
  • Author(s): Sogawa C, Abe A, Tsuji T, Koizumi M, Saga T,Kunieda T
  • Journal Title: Am.J.Pathol Volume: 177巻 Issue: 2 Pages: 822-828
  • DOI: 10.2353/ajpath.2010.091278
  • Peer Reviewed
• [Journal Article] Gastrointestinal Tract Disorder in Natriuretic Peptide Receptor B Gene Mutant Mice. (2010)
  • Author(s): Sogawa C.
  • Journal Title: Am.J.Pathol. Volume: 177 Pages: 822-828
  • Peer Reviewed
• [Presentation] 骨成長をもたらす突然変異(dwg)の原因遺伝子の同定 (2012)
  • Author(s): 山田郁・辻 岳人
  • Organizer: 第30回日本骨代謝学会
  • Place of Presentation: 東京
  • Year and Date: 2012-07-21
• [Presentation] 矮小を呈するstbマウスの組織学的解析および原因遺伝子のマッピング (2012)
  • Author(s): 岩上 大毅・辻 岳人
  • Organizer: 第30回日本骨代謝学会
  • Place of Presentation: 東京
  • Year and Date: 2012-07-21
• [Presentation] Roles of Syntaxin2/Epimorphin (Stx2/Epim) in progression of meiosis during mouse spermatogenesis (2011)
  • Author(s): Yasuhiro Fujiwara, その他
  • Organizer: 2^<nd> World Congress on Reproductive Biology
  • Place of Presentation: Cairns, Australia
  • Year and Date: 2011-10-10
• [Presentation] sks突然変異マウスのパキテン期精母細胞における核の形態異常 (2011)
  • Author(s): 梶田晋平, その他
  • Organizer: 第58回日本実験動物学会総会
  • Place of Presentation: 東京
  • Year and Date: 2011-05-26
• [Presentation] The role of Stntaxin2/Epimorphin (Stx2/Epinm) in progression of meisis during spermatogenesis. (2010)
  • Author(s): Fujiwara Y
  • Organizer: Society for the study of reproduction 43rd annual meeting
  • Place of Presentation: Milwaukee
  • Year and Date: 2010-07-30
• [Presentation] Cloning of candidate genes for TMEM48 binding proteins, which could be involved in gametogenesis. (2010)
  • Author(s): Kajita S
  • Organizer: Society for the study of reproduction 43rd annual meeting
  • Place of Presentation: Milwaukee
  • Year and Date: 2010-07-30
• [Presentation] repro34突然変異マウスを用いたStx2/Epim遺伝子の精子形成における機能の解析 (2010)
  • Author(s): 藤原靖浩
  • Organizer: 第57回日本実験動物学会
  • Place of Presentation: 京都
  • Year and Date: 2010-05-12
• [Presentation] マウス精巣においてTMEM48/NDC1と相互作用するタンパク質の探索 (2010)
  • Author(s): 梶田晋平
  • Organizer: 第57回日本実験動物学会
  • Place of Presentation: 京都
  • Year and Date: 2010-05-12