In vitro and in vivo analyses of differentiation/proliferation mechanism of the Theileria orientalis protozoan parasite
| Project/Area Number |
22580344
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Basic veterinary science/Basic zootechnical science
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| Research Institution | Azabu University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
KODAMA Michi 北海道大学, (連合)獣医学研究科, 客員研究員 (90466467)
KISHIKAWA Seigou 麻布大学, 環境保健学部, 教授 (10099377)
HISAMATSU Shin 麻布大学, 環境保健学部, 講師 (10198997)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 寄生虫 / Theileria orientalis / 原虫 / SCID-Bo / マクロファージ / theireria orientalis / Bo-RBC-SCID / BO-RBC-SCID |
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| Research Abstract |
Theileria orientalis（T.o）parasite infection cell lines were established from adherent cells of the peripheral blood of the bovine which was infected with T.o protozoan parasite. The cell lines were derived from monocytes/macrophages. After detection of T.o in the infected cell lines by the nested-PCR method, the SCID mice were substituted for bovine red blood cells (Bo-RBC-SCID), and transfected with T.o infected cells. As a result, piroplasms were detected in red blood cells in the peripheral blood of Bo-RBC-SCID. These findings suggest that T.o protozoan were subcultured in macrophage cells, differentiated and proliferated into the intraerythrocytic stage after having been infected with the bovine red blood cells in Bo-RBC-SCID.
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Report
(4 results)
Research Products
(10 results)
