Highly Assay of Autoantigens in BSE Sera for a Diagnosis
| Project/Area Number |
22580353
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied veterinary science
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| Research Institution | Rakuno Gakuen University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
今岡 進 関西学院大学, 理工学部, 教授 (60145795)
鈴木 一由 酪農学園大学, 獣医学部, 准教授 (30339296)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | BSE / 生前診断 / GFAP / 血清蛋白質 / 質量分析 / 血清診断 / 自己抗体 / 抗原検出 |
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| Research Abstract |
Our objective is to detect a serum biomarker Glial Fibrillary Acidic Protein (GFAP) of Bovine Spongiform Encephalopathy (BSE) by the Selected Reaction Monitoring (SRM) of quadrupole MS analysis from a bovine serum. SRM conditions were determined from the data of MS/MS fragmentation and identification of the protein by data dependent MS/MS analysis of Q-pole MS. An additive GFAP was highly recovered by the preparation method using alkylation of the proteins and removal of the albumin from the sera. SRM methods reported in this study could identify protein and peptide with high selectivity and sensitivity, therefore this method was useful for screening of known proteins and peptides.
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Report
(4 results)
Research Products
(25 results)
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[Journal Article] Neutrophil elastase inhibitor improves survival of rats with clinically relevant sepsis2010
Author(s)
Suda K, Takeuchi H, Hagiwara T, Miyasho T, Okamoto M, Kawasako K, Yamada S, Suganuma K, Wada N, Saikawa Y, Fukunaga K, Funakoshi Y, Hashimoto S, Yokota H, Maruyama I, Ishizaka A,Kitagawa Y
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Journal Title
Shock. May
Volume: 33(5):526-531
Related Report
Peer Reviewed
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[Journal Article] Protective Effect of High-Mobility Group Box 1 Blockade on Acute Liver Failure in Rats :(HMGB 1 Blockade for Rat Acute Liver Failure).Y.2010
Author(s)
Takano K, Shinoda M, Tanabe M, Miyasho T, Yamada S, Ono S, Masugi Y, Suda K, Fukunaga K, Hayashida T, Hibi T, Obara H, Takeuchi H, Kawachi S, Kawasako K, Okamoto M, Yokota H, Maruyama I, Kitagawa
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Journal Title
Shock
Volume: 34
Pages: 573-579
Related Report
Peer Reviewed
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[Journal Article] Spherical sulfated cellulose adsorbs high-mobility-group box chromosomal protein 1 in vitro and in vivo.2010
Author(s)
Suda K, Takeuchi H, Hagiwara T, Miyasho T, Yamada S, Inoue T, Okamoto M, Wada N, Saikawa Y, Fukunaga K, Hashimoto S, Yokota H, Maruyama I, Kitagawa Y.
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Journal Title
ASAIO J.
Volume: 56
Pages: 210-214
Related Report
Peer Reviewed
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[Journal Article] Neutrophil elastase inhibitor improves survival of rats with clinically relevant sepsis.2010
Author(s)
Suda K, Takeuchi H, Hagiwara T, Miyasho T, Okamoto M, Kawasako K, Yamada S, Suganuma K, Wada N, Saikawa Y, Fukunaga K, Funakoshi Y, Hashimoto S, Yokota H, Maruyama I, Ishizaka A, Kitagawa Y.
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Journal Title
Shock
Volume: 33
Pages: 526-531
Related Report
Peer Reviewed
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