Highly Assay of Autoantigens in BSE Sera for a Diagnosis

• Project/Area Number: 22580353
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied veterinary science
• Research Institution: Rakuno Gakuen University
• Principal Investigator: YOKOTA Hiroshi 酪農学園大学, 獣医学群, 教授 (90137414)
• Co-Investigator(Kenkyū-buntansha): 今岡 進 関西学院大学, 理工学部, 教授 (60145795) ; 鈴木 一由 酪農学園大学, 獣医学部, 准教授 (30339296)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: BSE / 生前診断 / GFAP / 血清蛋白質 / 質量分析 / 血清診断 / 自己抗体 / 抗原検出

Research Abstract

Our objective is to detect a serum biomarker Glial Fibrillary Acidic Protein (GFAP) of Bovine Spongiform Encephalopathy (BSE) by the Selected Reaction Monitoring (SRM) of quadrupole MS analysis from a bovine serum. SRM conditions were determined from the data of MS/MS fragmentation and identification of the protein by data dependent MS/MS analysis of Q-pole MS. An additive GFAP was highly recovered by the preparation method using alkylation of the proteins and removal of the albumin from the sera. SRM methods reported in this study could identify protein and peptide with high selectivity and sensitivity, therefore this method was useful for screening of known proteins and peptides.

Research Products

• [Journal Article] Accurate Determination of Tissue Steroid Hormones, Precursors and Conjugates in Adult Male Rat (2013)
  • Author(s): Maeda Naoyuki, Emi Tanaka, Tomokazu Suzuki, Kanako Okumura, Sachiko Nomura, Taku Miyasho, Satoko Haeno, and Hiroshi Yokota
  • Journal Title: J. Biochem. Volume: １５３ Pages: 63-71
  • Peer Reviewed
• [Journal Article] Nakade T.Proteome Analysis of Cerebrospinal Fluid in Healthy Beagle Dogs and Canine Encephalitis (2012)
  • Author(s): Nakamura K, Miyasho T, Nomura S, Yokota H
  • Journal Title: J Vet Med Sci Volume: 74 Pages: 751-756
  • Peer Reviewed
• [Journal Article] Particle-Induced X-ray Emission Analysis of Elements in Plasma from Wild and Captive Sea Turtles(Eretmochelys imbricata, Chelonia mydas, and Caretta caretta) in Okinawa (2012)
  • Author(s): Kazuyuki Suzuki & Jun Noda & Makio Yanagisawa & Isao Kawazu & Kouichiro Sera & Daisuke Fukui & Mitsuhiko Asakawa & Hiroshi Yokota
  • Journal Title: Japan Biol Trace Elem Res
  • Peer Reviewed
• [Journal Article] Relationships between Curved Carapace Sizes and Plasma Major and Trace Element Status in Captive Hawksbill Sea Turtle (Eretmochelys imbricata) (2012)
  • Author(s): Suzuki K, Noda J, Yanagisawa M, Kawazu I, Sera K, Fukui D, Asakawa M, Yokota H
  • Journal Title: J Vet Med Sci Volume: (In press)
  • Peer Reviewed
• [Journal Article] Relationships between Carapace Sizes and Plasma Major and Trace Element Status in Captive Hawksbill Sea Turtles (<i>Eretmochelys imbricata</i>) (2012)
  • Author(s): Kazuyuki SUZUKI*, Jun NODA, Makio YANAGISAWA, Isao KAWAZU, Kouichiro SERA, Daisuke FUKUI, Mitsuhiko ASAKAWA and Hiroshi YOKOTA.
  • Journal Title: Journal of Veterinary Medical Science Volume: 74 Issue: 12 Pages: 1677-1680
  • DOI: 10.1292/jvms.12-0099
  • NAID: 130001879676
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed
• [Journal Article] Proteome Analysis of Cerebrospinal Fluid in Healthy Beagles and Canine Encephalitis (2012)
  • Author(s): Nakamura K, Miyasho T, Nomura S, Yokota H, Nakade T
  • Journal Title: Journal of Veterinary Medical Science Volume: 74 Issue: 6 Pages: 751-756
  • DOI: 10.1292/jvms.11-0474
  • NAID: 130002090077
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed
• [Journal Article] Oxidatively Damaged Proteins in the Early Stage of Testicular Toxicities in Male Rats by Orally Administered with a Synthetic Oestrogen (2011)
  • Author(s): Yunlan Li, Kanako Okumura, Sachiko Nomura, Naoyuki Maeda, Taku Miyasho, Hiroshi Yokota
  • Journal Title: Diethylstilbestrol Reproductive Toxicol Volume: 31 Pages: 26-34
  • Peer Reviewed
• [Journal Article] High Mobility Group Box 1 (HMGB1) Protein Is Present in the Cerebrospinal Fluid of Dogs with Encephalitis (2011)
  • Author(s): Miyasho T, Nakamura K, Nomura S, Kawasako K, Nakade T, Yamada S, Yokota H
  • Journal Title: J Vet Med Sci Volume: 73(7) Pages: 917-922
  • NAID: 130000676922
  • Peer Reviewed
• [Journal Article] Simplified PCR Assay for Fast and Easy Mycoplasma Mastitis Screening in Dairy Cattle (2011)
  • Author(s): Higuchi H., Iwano H., Kawai K., Ohta T., Obayashi T., Hirose K., Ito N., Yokota H., Tamura Y. and Nagahata H. A
  • Journal Title: J. Vet Science Volume: 12,(2) Pages: 191-193
  • NAID: 120005761687
  • Peer Reviewed
• [Journal Article] ATP-binding cassette transporter isoform C2 localizes to the apical plasma membrane via interactions with scaffolding protein (2011)
  • Author(s): Emi Y , Nomura S, Yokota H, Sakaguchi M
  • Journal Title: J Biochem Volume: 149:177-189
  • NAID: 10030583422
  • Peer Reviewed
• [Journal Article] Oxidatively Damaged Proteins in the Early Stage of Testicular Toxicities in Male Rats by Orally Administered with a Synthetic Oestrogen, Diethylstilbestrol (2011)
  • Author(s): Yunlan Li, Kanako Okumura, Sachiko Nomura, Naoyuki Maeda, Taku Miyasho, Hiroshi Yokota
  • Journal Title: Reproductive Toxicol Volume: 31 Pages: 26-34
  • Peer Reviewed
• [Journal Article] ATP-binding cassette transporter isoform C2 (ABCC2) localizes to the apical plasma membrane via interactions with PDZK1 scaffolding protein (2011)
  • Author(s): Emi, Y., Nomura, S., Yokota, H., Sakaguchi, M.
  • Journal Title: Journal of Biochemistry Volume: 149 Issue: 2 Pages: 177-189
  • DOI: 10.1093/jb/mvq131
  • Peer Reviewed
• [Journal Article] A Simplified PCR Assay for Fast and Easy Mycoplasma Mastitis Screening in Dairy Cattle (2011)
  • Author(s): Higuchi H., Iwano H., Kawai K., Ohta T., Obayashi T., Hirose K., Ito N., Yokota H., Tamura Y., Nagahata H.
  • Journal Title: J.Vet Scienc Volume: 12 Issue: 1 Pages: 191-193
  • DOI: 10.1016/j.reprotox.2010.09.007
  • NAID: 120005761687
  • Peer Reviewed
• [Journal Article] Biochemical and immunohistochemical characterization of the amyloid in canine amyloid-producing odontogenic tumor (2010)
  • Author(s): Hirayama K, Miyasho T, Ohmachi T, Watanabe T, Yokota H, Taniyama H
  • Journal Title: Vet Pathol Volume: 47 Pages: 915-922
  • Peer Reviewed
• [Journal Article] Placental Transfer of Conjugated Bisphenol A and Subsequent Reactivation in the Rat fetus (2010)
  • Author(s): Miyu Nishikawa, Hidetomo Iwano, Risa Yanagisawa, Nanako Koike, Hiroki Inoue and Hiroshi Yokota
  • Journal Title: Environ. Health Perspect Volume: 118 Pages: 1196-1203
  • NAID: 120005761579
  • Peer Reviewed
• [Journal Article] Neutrophil elastase inhibitor improves survival of rats with clinically relevant sepsis (2010)
  • Author(s): Suda K, Takeuchi H, Hagiwara T, Miyasho T, Okamoto M, Kawasako K, Yamada S, Suganuma K, Wada N, Saikawa Y, Fukunaga K, Funakoshi Y, Hashimoto S, Yokota H, Maruyama I, Ishizaka A,Kitagawa Y
  • Journal Title: Shock. May Volume: 33(5):526-531
  • Peer Reviewed
• [Journal Article] Placental Transfer of Conjugated Bisphenol A and Subsequent Reactivation in the Rat fetus (2010)
  • Author(s): Miyu Nishikawa, Hidetomo Iwano, Risa Yanagisawa, Nanako Koike, Hiroki Inoue, Hiroshi Yokota
  • Journal Title: Environ.Health Perspect. Volume: 118 Pages: 1196-1203
  • NAID: 120005761579
  • Peer Reviewed
• [Journal Article] Protective Effect of High-Mobility Group Box 1 Blockade on Acute Liver Failure in Rats :(HMGB 1 Blockade for Rat Acute Liver Failure).Y. (2010)
  • Author(s): Takano K, Shinoda M, Tanabe M, Miyasho T, Yamada S, Ono S, Masugi Y, Suda K, Fukunaga K, Hayashida T, Hibi T, Obara H, Takeuchi H, Kawachi S, Kawasako K, Okamoto M, Yokota H, Maruyama I, Kitagawa
  • Journal Title: Shock Volume: 34 Pages: 573-579
  • Peer Reviewed
• [Journal Article] Biochemical and immunohistochemical characterization of the amyloid in canine amyloid-producing odontogenic tumor (2010)
  • Author(s): Hirayama K, Miyasho T, Ohmachi T, Watanabe T, Yokota H, Taniyama H
  • Journal Title: Vet Pathol. Volume: 47 Pages: 915-922
  • Peer Reviewed
• [Journal Article] Spherical sulfated cellulose adsorbs high-mobility-group box chromosomal protein 1 in vitro and in vivo. (2010)
  • Author(s): Suda K, Takeuchi H, Hagiwara T, Miyasho T, Yamada S, Inoue T, Okamoto M, Wada N, Saikawa Y, Fukunaga K, Hashimoto S, Yokota H, Maruyama I, Kitagawa Y.
  • Journal Title: ASAIO J. Volume: 56 Pages: 210-214
  • Peer Reviewed
• [Journal Article] Neutrophil elastase inhibitor improves survival of rats with clinically relevant sepsis. (2010)
  • Author(s): Suda K, Takeuchi H, Hagiwara T, Miyasho T, Okamoto M, Kawasako K, Yamada S, Suganuma K, Wada N, Saikawa Y, Fukunaga K, Funakoshi Y, Hashimoto S, Yokota H, Maruyama I, Ishizaka A, Kitagawa Y.
  • Journal Title: Shock Volume: 33 Pages: 526-531
  • Peer Reviewed
• [Presentation] Selected Reaction Monitoring(SRM)を用いたウシ血中グリア専制生賛成蛋白質の検知 (2011)
  • Author(s): 鈴木智和、田中絵美、前田尚之、宮庄拓、横田博
  • Organizer: 日本質量分析学会
  • Place of Presentation: 阪急エキスポパーク
  • Year and Date: 2011-09-15
• [Presentation] 臓器灌流モデルを用いたビスフェノールA体内動態の解明 (2010)
  • Author(s): 井上博紀, 西川美宇, 佐野千枝, 太田有耶, 櫻田泰樹, 岩野英知, 横田博
  • Organizer: 環境ホルモン学会
  • Place of Presentation: 東京大学
  • Year and Date: 2010-12-17
• [Presentation] メタボローム解析によるビスフェノールAの妊娠期暴露影響の新規探索 (2010)
  • Author(s): 岩野英知, 西川美宇, 森田安奈, 柳沢梨沙, 封馬澄人, 井上博紀, 横田博
  • Organizer: 環境ホルモン学会
  • Place of Presentation: 東京大学
  • Year and Date: 2010-12-17
• [Presentation] Selected ReactionMonitoring (SRM)を用いた牛血中グリア繊維性酸性蛋白質の検知
  • Author(s): 鈴木智和1・ 田中絵美1・ 前田尚之1,2・宮庄 拓2・ 横田 博2 (1食肉科研 ・2酪農大獣生化)
  • Organizer: 第59回日本質量分析学会(大阪)