lnvestigation of rapid diagnosis and prevent for protothecal mastitis

• Project/Area Number: 22580368
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Clinical veterinary science
• Research Institution: Nihon University
• Principal Investigator: KANO Rui 日本大学, 生物資源科学部, 准教授 (00318388)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 農林水産学 / 獣医学 / 乳牛 / 乳房炎 / ﾌﾟﾛﾄﾃｶ / genotype / 遺伝子タイピング / プロトテカ乳房炎 / 防除法 / 消毒剤 / プロトテカ / 藻類 / PCR / 遺伝子診断 / 薬剤感受性試験 / Genotype / Prototheca zopfii

Research Abstract

Bovine mastitis due to Prototheca zopfii leads to reduced milk production and is difficult to cure. Therefore, preventlon is the best approach and this is best achieved through theuse of effective antifugal drugs and disinfectants. The 18S rDNA sequences of diverse clinical speclmens from different areas in Japan were studied to clarify the pathogenicity of P zopLfii var. zopfii.All isolates from bovine mastitis in Japan were identified as.P. zopfii genotype 2. Therefore, P zopfii var.zopfii genotype 2 is associated with bovine mastitis.

Research Products

• [Journal Article] Short communication:Molecular typingof Prototheca zopfii from bovine mastitis inJapan. (2012)
  • Author(s): Sobukawa H, Yamaguchi S, Kano R, Ito T,Suzuki K, Onozaki M, Hasegawa A, Kamata H.
  • Journal Title: J. Daity. Sci. Volume: 95 Pages: 4442-4426
  • Peer Reviewed
• [Journal Article] Short communication: Molecular typing of Prototheca zopfii from bovine mastitis in Japan (2012)
  • Author(s): Sobukawa H, Yamaguchi S, Kano R, Ito T, Suzuki K, Onozaki M, Hasegawa A, Kamata H.
  • Journal Title: Journal of Daily Science Volume: 95 Issue: 8 Pages: 4442
  • DOI: 10.3168/jds.2011-5101
  • URL: https://pure.teikyo.jp/en/publications/c11d06f9-539f-4354-9295-9d4037f12e48
  • Peer Reviewed
• [Journal Article] Experimental Infection of Bovine Mammary Gland with Prototheca zopfii Genotype l. (2011)
  • Author(s): Ito T, Kano R, Sobukawa H, Ogawa J, Honda Y,Hosoi Y, Shibuya H, Sato T, Hasegawa A,Kamata H.
  • Journal Title: J. Vet. Med.、Sci. Volume: 73 Pages: 117-119
  • URL: http://www.ncbi.nlm.nih.govipubmed/20736514
  • Peer Reviewed
• [Journal Article] In vitro susceptibility of Prototheca zopfii genotypes 1 and 2 (2011)
  • Author(s): Sohukawa H, Kano R, Ito T, Onozaki M,Makimura K, Hasegawa A, Kamata H.
  • Journal Title: Med. MycoL Volume: 49 Issue: 2 Pages: 222
  • DOI: 10.3109/13693786.2010.511285
  • URL: https://pure.teikyo.jp/en/publications/9bb05baa-7934-4bdf-b741-858480a91ac5
  • Peer Reviewed
• [Journal Article] In vitro algaecide effect of disinfectants on Prototheca ZOPfii genotypes 1 and 2. (2011)
  • Author(s): Sobukawa H, Watanabe M, Kano R, Ito T,Onozaki M, Hasegawa A, Kamata H.
  • Journal Title: J. Vet.Med Sci Volume: 15 Pages: 1527-1529
  • NAID: 130000862402
  • URL: http://www.ncbi.nlm.nih.gov/pubmed/217379
  • Peer Reviewed
• [Journal Article] In vitro Algaecide Effect of Disinfectants on Prototheca zopfii Genotypes 1 and 2 (2011)
  • Author(s): Sobukawa H, Watanabe M, Kano R, Ito T, Onozaki M, Hasegawa A, Kamata H
  • Journal Title: Journal of Veterinary Medical Science Volume: 73 Issue: 11 Pages: 1527-1529
  • DOI: 10.1292/jvms.11-0256
  • NAID: 130000862402
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed
• [Journal Article] Experimental Infection of Bovine Mammary Gland with Prototheca zopfii Genotype 1 (2011)
  • Author(s): Ito T, Kano R, Sobukawa H, Ogawa J, Honda Y, Hosoi Y, Shibuya H, Sato T, Hasegawa A, Kamata H.
  • Journal Title: Journal of Veterinary Medical Science Volume: 73 Pages: 117-119
  • NAID: 130000307713
  • Peer Reviewed
• [Journal Article] In vitro algaecid effect of disinfectants on Prototheca zopfii genotypes 1 and 2 (2011)
  • Author(s): Sobukawa H, Watanabe M, Kano R, Ito T, Onozaki M, Hasegawa A, Kamata H.
  • Journal Title: Medical Mycology Volume: 49 Pages: 222-224
  • NAID: 130000862402
  • Peer Reviewed
• [Presentation] 本邦から分離されたPrototheca zopfiiの分子疫学的調査 (2012)
  • Author(s): 曽布川英人、山口修平、加納塁.伊藤隆晶、鎌田寛
  • Organizer: 第154回日本獣医学科学術集会
  • Place of Presentation: 岩手大学
• [Remarks] 日本大学生物資源科学部獣医学科
  • URL: http://hp..brs.nihon-u.ac.jP/-vethome/
• [Remarks] 日本大学生物資源科学部獣医学科
  • URL: http://hp.brs.nihon-u.ac.jp/~vethome/