| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Research Abstract |
In this study, we developed the RNAi vector system which expressed siRNA in cytoplasm using T7 RNA polymerase. The co-transfection of pCMV-T7RNAP and pT7-RNAi-Lu significantly suppressed luciferase expression by pGL3-control plasmid in HeLa cells and A549 cells. Moreover, the co-transfection of pCMV-T7RNAP and pT7-RNAi-p53 significantly suppressed p53 protein expression in HeLa cells. Thus, we were able to confirm that the new vector system using T7 RNA polymerase has function to suppress the expression of target gene.
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