Study on some proteins controling cell death in oxidative stress-induced neuronal cell death.
| Project/Area Number |
22590091
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biological pharmacy
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| Research Institution | Suzuka University of Medical Science |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
INOUE Sumiko 鈴鹿医療科学大学, 薬学部, 准教授 (20378657)
HASHI Minako 金沢大学, 医学系研究科, 研究員(講師) (10272957)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | グルタミン酸 / 細胞死 / アポトーシス / プロテオミクス / シグナル伝達 / ストレス / タンパク質 / 脳神経疾患 / GRP-78 / Galectin-1 / プロテオミクス解析 / 老化 / プロテインキナーゼC / シグナル伝津 |
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| Research Abstract |
We have reported that glutamate exerts cytotoxic action through inhibition of cystine uptake, which leads to a marked decrease in cellular glutathione (GSH) levels exposing the cells to oxidative stresses leading to cell death. This cell death seemed to be apoptosis accompanying with 1-2Mbp giant DNA fragmentation led to internucleosomal DNA fragmentation. We expand on recent studies of intracellular proteins by examining the cell death regulating proteins or factors by using two-dimensional differential in-gel electrophoresis (2D-DIGE) in the intracellular peptides from cell extracts of C6 rat glioma cells. We identified a protein kinase C-δas one of protein kinases involved in the glutamate-induced cell death, and furthermore, identified 3 peptides derived from 10 proteins with LC-MS/MS in theglutamate-treated cells. Three peptides such as 78-kDa glucose-regulated protein (GRP78), annexin -A5and galectin-1 were increased in glutamate -treated glioma cells.
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Report
(4 results)
Research Products
(23 results)
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[Presentation]2010
Author(s)
Higuchi, Y. Hashii, M., Tanii, H. and Kohriyama, Y.
Organizer
16th World Congress of Basic and Clinical Pharmacology
Place of Presentation
コペ ンハーゲン
Year and Date
2010-07-20
Related Report
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[Presentation] Effect of zonisamide on apoptosis-related factors formed during iron-induced epileptogenesis in rat brain.
Author(s)
Higuchi, Y., Furukawa, A., Oowada, S., Komatsu, M. and Hiramatsu, M.,
Organizer
14th International Congress of Histochemistry and Cytochemistry (ICHC 2012),
Place of Presentation
京都市
Related Report
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