| Project/Area Number |
22590091
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biological pharmacy
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| Research Institution | Suzuka University of Medical Science |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
INOUE Sumiko 鈴鹿医療科学大学, 薬学部, 准教授 (20378657)
HASHI Minako 金沢大学, 医学系研究科, 研究員(講師) (10272957)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | グルタミン酸 / 細胞死 / アポトーシス / プロテオミクス / シグナル伝達 / ストレス / タンパク質 / 脳神経疾患 / GRP-78 / Galectin-1 / プロテオミクス解析 / 老化 / プロテインキナーゼC / シグナル伝津 |
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| Research Abstract |
We have reported that glutamate exerts cytotoxic action through inhibition of cystine uptake, which leads to a marked decrease in cellular glutathione (GSH) levels exposing the cells to oxidative stresses leading to cell death. This cell death seemed to be apoptosis accompanying with 1-2Mbp giant DNA fragmentation led to internucleosomal DNA fragmentation. We expand on recent studies of intracellular proteins by examining the cell death regulating proteins or factors by using two-dimensional differential in-gel electrophoresis (2D-DIGE) in the intracellular peptides from cell extracts of C6 rat glioma cells. We identified a protein kinase C-δas one of protein kinases involved in the glutamate-induced cell death, and furthermore, identified 3 peptides derived from 10 proteins with LC-MS/MS in theglutamate-treated cells. Three peptides such as 78-kDa glucose-regulated protein (GRP78), annexin -A5and galectin-1 were increased in glutamate -treated glioma cells.
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