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Studies on resource of Ephedra sinica and the related Ephedra plants in Eurasia and evaluation of their quality as the crude drug.

Research Project

Project/Area Number 22590116
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Environmental pharmacy
Research InstitutionKyushu University of Health and Welfare

Principal Investigator

KAKIUCHI Nobuko  九州保健福祉大学, 薬学部, 教授 (30204324)

Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords生薬 / 資源 / DNA 解析 / ユーラシア大陸 / エフェドラ・シニカ / DNA解析 / エフェドラ・ゲラルディアーナ / ネパール
Research Abstract

The aerial part of Ephedra plants is a staple crude drug worldwide. Among them, E. sinica iscurrently the most commonly used crude drug in the Japanese and Chinese market. E. dahuricaand E. distachya are believed to be closely related to E. sinica. However, E. dahurica,distributed in limited area of Far East Russia, has no record of medicinal usage, neither clearevidence on the relationship with E. sinica. I examined DNA sequences of E. dahurica samples,which I had collected in Bryatia, East Siberia, in 2006, E. sinica vouchers of the Herbaria ofKanazawa University collected in China and Mongolia by Prof Mikage. I also examined E.distachya samples collected in 2008 in Alps as well as vouchers of Kassel University depositedby Prof Freitag. I revealed the phylogenic relationship of E. sinica-relating Ephedra plants, andevaluated them as the crude drug.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (45 results)

All 2013 2012 2011 2010

All Journal Article (25 results) (of which Peer Reviewed: 21 results) Presentation (20 results)

  • [Journal Article] Phylogenic study on Ephedra plants related to Ephedra sinica by sequence analysis of internal transcribed spacer 2 region (ITS2) in nuclear ribosomal DNA.2013

    • Author(s)
      Kakiuchi N, Higuchi M,Tanaka S, Nishikawa K, Kamikawa S, Ohtsuka I,Freitag H.
    • Journal Title

      J KUHW

      Volume: 14 Pages: 169-172

    • NAID

      110009596035

    • Related Report
      2012 Final Research Report
  • [Journal Article] Phylogenic study on Ephedra plants related to Ephedra sinica by sequence analysis of internal transcribed spacer 2 region (ITS2) in nuclear ribosomal DNA.2013

    • Author(s)
      Kakiuchi N
    • Journal Title

      J. KUHW

      Volume: 14 Pages: 169-172

    • NAID

      110009596035

    • Related Report
      2012 Annual Research Report
  • [Journal Article] Synthesis of a New Glycosphingolipid from the Marine Ascidian Microcosmus sulcatus using a One-Pot Glycosylation Strategy2013

    • Author(s)
      Ohtsuka I
    • Journal Title

      Tetrahedron

      Volume: 69 Issue: 5 Pages: 1470-1475

    • DOI

      10.1016/j.tet.2012.12.023

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Phylogenic examination of crude drugs derived from Yunnanese Swertia plants.2013

    • Author(s)
      Kakiuchi N
    • Journal Title

      J Nat Med

      Volume: 67 Issue: 1 Pages: 206-210

    • DOI

      10.1007/s11418-013-0776-x

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Difference in cultivation characteristics and genetic polymorphism between Chinese and Japanese strains of Wolfiporia cocos Ryvarden et Gilbertson (Poria cocos Wolf).2012

    • Author(s)
      Kobira S,Atsumi T, Kakiuchi N, Mikage M.
    • Journal Title

      J Nat Med

      Volume: 66 Pages: 493-499

    • NAID

      10031087787

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Quality evaluation of Chotoko - Local and specific variations in the alkaloid contents of Uncaria plants-.2012

    • Author(s)
      Shi W, Kitaoka F, Doui M,Miyake K, Sasaki Y, Kakiuchi N, Mikage M.
    • Journal Title

      J Trd Med

      Volume: 29 Pages: 63-73

    • NAID

      120006547501

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Chemical changes in rhubarb heated after processed with liquor.2012

    • Author(s)
      Doui M, Kakiuchi N,Miakge M.
    • Journal Title

      J Trad Med.

      Volume: 27 Pages: 143-147

    • NAID

      130004495561

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Quality evaluation of <I>Chotoko</I><BR>- Local and specific variations in the alkaloid contents of <I>Uncaria</I> plants -2012

    • Author(s)
      Shi, W
    • Journal Title

      Journal of Traditional Medicines

      Volume: 29 Issue: 2 Pages: 63-73

    • DOI

      10.11339/jtm.29.63

    • NAID

      130004942392

    • ISSN
      1880-1447, 1881-3747
    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Establishment of Synthesis Method of Molecular Probe Containing Carbohydrate Ligand and Photoreactive Group for Elucidation of Carbohydrate – Lectin Interactions by Glycosyl Trichloroacetoimidate Strategy2012

    • Author(s)
      Ohtsuka I
    • Journal Title

      J. KUHW

      Volume: 14 Pages: 165-168

    • Related Report
      2012 Annual Research Report
  • [Journal Article] A molecular phylogenetic study of the Ephedra distachya / E. sinica complex in Eurasia.2011

    • Author(s)
      Kakiuchi N, Mikage M, Ickert-Bond S,Maier-Stolte M, Freitag H.
    • Journal Title

      Willdenowia

      Volume: 41 Pages: 203-215

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Molecular Genetic Characteristics of Nepalese Ephedra Plants.2011

    • Author(s)
      Hamanaka E, Ohkubo K, Mikage M and Kakiuchi N.
    • Journal Title

      J Jpn Bot.

      Volume: 86 Pages: 303-313

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Temporal change in quality of a Kampo decoction packed by a decocting machine (1)2011

    • Author(s)
      Doui M, Anjiki N, Ina S, Yoshimitsu M,Kawahara N, Goda Y, Kakiuchi N, Mikage M.
    • Journal Title

      Shoyakugaku Zasshi

      Volume: 65 Pages: 103-107

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] The relationship between growth of the aerial part and alkaloid content variation in cultivated Aconitum carmichaeli Debeaux.2011

    • Author(s)
      Kawasaki R, Motoya W, At sumi T, Mouri C, Kakiuchi N, Mikage M.
    • Journal Title

      J Nat Med.

      Volume: 65 Pages: 111-5

    • NAID

      10029460564

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] A molecular phylogenetic study of the Ephedra distachya/E.sinica complex in Eurasia2011

    • Author(s)
      Nobuko Kakiuchi, Masayuki Mikage, 他3名
    • Journal Title

      Willdenowia

      Volume: 41 Issue: 2 Pages: 203-215

    • DOI

      10.3372/wi.41.41201

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular Genetic Characteristics of Nepalese Ephedra Plants2011

    • Author(s)
      Hamanaka, E., Ohkubo, K., Mikage, M., Kakiuchi, N.
    • Journal Title

      J.Jpn.Bot.

      Volume: 86(Ephedraceae) Pages: 303-313

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Temporal change in quality of a Kampo decoction packed by a decocting machine2011

    • Author(s)
      Doui M., Anjiki N., Ina S., Yoshimitsu M., Kawahara N., Goda Y., Kakiuchi N., Mikage M.
    • Journal Title

      Shoyakugaku Zasshi

      Volume: 65 Pages: 103-107

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 附子の栽培研究-アルカロイド含量が安定した附子製造原料作出のための条件2011

    • Author(s)
      御影雅幸, 松山和寛, 川崎亮一, 垣内信子
    • Journal Title

      薬用植物研究

      Volume: 33 Pages: 1-6

    • NAID

      40022285025

    • Related Report
      2011 Annual Research Report
  • [Journal Article] The effect of structural differences in the reducing terminus of sugars on the binding affinity of carbohydrates and proteins analyzed using photoaffinity labeling2011

    • Author(s)
      Ohtsuka, I., Sadakane, Y., Higuchi, M., Hada, N., Hada, J., Kakiuchi N., Sakushima, A.
    • Journal Title

      Bioorg.Med.Chem.

      Volume: 19 Pages: 894-899

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The relationship between growth of the aerial part and alkaloid content variation in cultivated Aconitum carmichaeli Debeaux2011

    • Author(s)
      Kawasaki, R.,・Motoya, W., Atsumi, T.,・Mouri, C.,・Kakiuchi, N.,・Mikage, M.
    • Journal Title

      J Nat Med

      Volume: 65 Issue: 1 Pages: 111-115

    • DOI

      10.1007/s11418-010-0466-x

    • NAID

      10029460564

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A herbological studies on medicinal effects and employments of rhubarb processed with liquor (2). Comparison of principal compound contents in liquor-dipped rhubarb and liquor-soaked rhubarb.2010

    • Author(s)
      Doui M, Kakiuchi N,Ebara T, Miakge M.
    • Journal Title

      Kampo Med.

      Volume: 61 Pages: 133-137

    • NAID

      10026477146

    • Related Report
      2012 Final Research Report 2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Herbological studies on combination of rhubarb and mirabilite2010

    • Author(s)
      Doui M, Ando H, Goi C, Kakiuchi N, Mikage M.
    • Journal Title

      Yakushigaku Zasshi.

      Volume: 45 Pages: 106-11

    • NAID

      10029416323

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Studies of Ephedra plants in Asia. Part 6:Geographical changes of anatomical features and alkaloids content of Ephedra sinica.2010

    • Author(s)
      Wang LL,Kakiuchi N, Mikage M.
    • Journal Title

      J Nat Med.

      Volume: 64 Pages: 63-9

    • NAID

      10027709124

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Studies on Ephedra plants in Asia. Part 6 : Geographical changes of anatomical features and alkaloids content of Ephedra sinica2010

    • Author(s)
      L.Wang, N.Kakiuchi, M.Miakge
    • Journal Title

      J.Nat.Med

      Volume: 64 Pages: 63-69

    • NAID

      10027709124

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 「大黄」の古来の煎じ時間に関する考察2010

    • Author(s)
      堂井美里, 江原利彰, 五井千尋, 安藤広和, 垣内信子, 御影雅幸
    • Journal Title

      Kampo Med.

      Volume: 61 Pages: 282-288

    • NAID

      10030705121

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Chemical changes in rhubarb heated after processed with liquor.2010

    • Author(s)
      M.Doui, N.Kakiuchi, M.Miakge
    • Journal Title

      J.Trad Med.

      Volume: 27 Pages: 143-147

    • NAID

      130004495561

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] 九州産トリカブトの資源調査2012

    • Author(s)
      渥美聡孝,大塚功,垣内信子
    • Organizer
      第29回日本薬学会九州支部大会
    • Place of Presentation
      熊本
    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Presentation] アジア及び地中海沿岸地域に分布するEphedragerardianaおよび関連Ephedra属植物の系統解析2012

    • Author(s)
      垣内信子,渥美聡孝,大塚功,御影雅幸,H.Freitag,M.Maier-Stolte
    • Organizer
      日本生薬学会第59回年会
    • Place of Presentation
      千葉.
    • Related Report
      2012 Final Research Report
  • [Presentation] 南北海道に自生するトリカブト植物の遺伝子タイピングと含有成分を指標とした識別2012

    • Author(s)
      垣内信子,樋口真理,上川翔平,大塚功,渥美聡孝,山岸喬,西澤信,林茂樹,菱田敦之
    • Organizer
      日本薬学会第132年会
    • Place of Presentation
      札幌.
    • Related Report
      2012 Final Research Report
  • [Presentation] Synthesis of New Molecular Tools for Elucidation of Carbohydrates Roles by Photoaffinity Labeling ~Carbohydrate – Protein Interactions are Affected by Different of Glycosidic Formations and Conformation of Reducing End Sugar~2012

    • Author(s)
      大塚功
    • Organizer
      26th International Carbohydrate Symposium
    • Place of Presentation
      Madrid
    • Related Report
      2012 Annual Research Report
  • [Presentation] 天然由来糖脂質の合成から糖鎖機能を探る~脊索動物リモーニ・ディ・マーレ由来糖脂質の合成2012

    • Author(s)
      大塚功
    • Organizer
      第31回日本糖質学会年会
    • Place of Presentation
      鹿児島
    • Related Report
      2012 Annual Research Report
  • [Presentation] アジア及び地中海沿岸地域に分布するEphedra gerardianaおよび関連Ephedra属植物の系統解析2012

    • Author(s)
      垣内信子
    • Organizer
      日本生薬学会第59回年会
    • Place of Presentation
      千葉
    • Related Report
      2012 Annual Research Report
  • [Presentation] 脊索動物リモーニ・ディ・マーレ由来糖脂質の合成2012

    • Author(s)
      大塚功
    • Organizer
      第29回日本薬学会九州支部大会
    • Place of Presentation
      熊本
    • Related Report
      2012 Annual Research Report
  • [Presentation] 薬用Clematis属植物の研究(1)威霊仙のDNA研究2011

    • Author(s)
      韓燕、真野真澄、北岡文代、姜東孝、垣内信子、御影雅幸
    • Organizer
      日本生薬学会第58年会
    • Place of Presentation
      昭和大学薬学部(東京都)
    • Year and Date
      2011-09-24
    • Related Report
      2011 Annual Research Report
  • [Presentation] ユーラシア大陸に自生するEphedra sinica関連マオウ属植物のDNA解析と系統解析2011

    • Author(s)
      樋口真理、田中沙織、西川佳奈美、上川翔平、大塚功、垣内信子
    • Organizer
      日本生薬学会第58年会
    • Place of Presentation
      昭和大学薬学部(東京都)
    • Year and Date
      2011-09-24
    • Related Report
      2011 Annual Research Report
  • [Presentation] ネパール産マオウ属植物に関する新知見2011

    • Author(s)
      御影雅幸、三宅克典、北岡文代、垣内信子
    • Organizer
      植物地理・分類学会2011年度大会
    • Place of Presentation
      京都大学楽友会館(京都府)
    • Year and Date
      2011-06-04
    • Related Report
      2011 Annual Research Report
  • [Presentation] 光アフィニティーラベル法による新規分子ツールの開発-糖鎖結合様式が認識に及ぼす影響を検証2011

    • Author(s)
      大塚功、定金豊、樋口真理、垣内信子
    • Organizer
      日本薬学会第131年会
    • Place of Presentation
      ツインメッセ静岡(静岡県)
    • Year and Date
      2011-03-31
    • Related Report
      2011 Annual Research Report
  • [Presentation] ネパール産マオウ属植物に関する新知見2011

    • Author(s)
      御影雅幸,三宅克典,北岡文美代,垣内信子
    • Organizer
      植物地理・分類学会
    • Place of Presentation
      京都
    • Related Report
      2012 Final Research Report
  • [Presentation] ユーラシア大陸に自生するEphedrasinica関連マオウ属植物のDNA解析と系統解析2011

    • Author(s)
      樋口真理,田中沙織,西川佳奈美,上川翔平,大塚功,垣内信子,御影雅幸,H.Freitag
    • Organizer
      第58回日本生薬学会
    • Place of Presentation
      東京
    • Related Report
      2012 Final Research Report
  • [Presentation] 薬用Clematis属植物の研究(1)-「威霊仙」のDNA解析-2011

    • Author(s)
      韓燕,間野真澄,北岡文美代,姜東孝,垣内信子,御影雅幸
    • Organizer
      日本生薬学会第58年会
    • Place of Presentation
      東京.
    • Related Report
      2012 Final Research Report
  • [Presentation] 中国産茯苓と日本産茯苓の栽培特性およびDNAの比較2010

    • Author(s)
      小平早百合,御影雅幸、垣内信子
    • Organizer
      第130回日本薬学会
    • Place of Presentation
      岡山.
    • Related Report
      2012 Final Research Report
  • [Presentation] The relationship of alkaloid contents between daughter tuberous root and rootlet of Aconitium carmichaeli.2010

    • Author(s)
      Kawasaki R, Oi I, Miyake K, Kakiuchi N, Mikage M.
    • Organizer
      The 5th JSP-CCTNM-KSP Joint Symposium on Pharmacognosy.
    • Place of Presentation
      徳島
    • Related Report
      2012 Final Research Report
  • [Presentation] The relationship of alkaloid contents between daughter tuberous root and rootlet of Aconitium carmichaeli.2010

    • Author(s)
      R.Kawasaki, I.Oi, K.Miyake, N.Kakiuchi, M.Mikage
    • Organizer
      The 5th JSP-CCTNM-KSP Joint Symposium on Pharmacognosy
    • Place of Presentation
      徳島
    • Related Report
      2010 Annual Research Report
  • [Presentation] Studies on processing for Rhubarb.2010

    • Author(s)
      M.Doui, N.Kakiuchi, M.Mikage
    • Organizer
      The 5th JSP-CCTNM-KSP Joint Symposium on Pharma-cognosy
    • Place of Presentation
      徳島
    • Related Report
      2010 Annual Research Report
  • [Presentation] 「釣藤鉤」の研究(1)-Uncaria属植物のDNA研究2010

    • Author(s)
      施ぶん、北岡文代、堂井美里、御影雅幸、垣内信子、小山鐵夫、渡邊高志
    • Organizer
      日本生薬学会57回年会
    • Place of Presentation
      徳島
    • Related Report
      2010 Annual Research Report
  • [Presentation] Synthesis of New Molecular Tools for Elucidation of Carbohydrates Roles by Photoaffinity Labeling~Carbohydrate-Protein Interactions are Affected by Difference in Glycosidic Formations2010

    • Author(s)
      I.Ohtsuka, Y.Sadakane, M.Higuchi, N.Hada, J.Hada, N.Kakiuchi, A.Sakushima
    • Organizer
      International Chemical Congress of Pacific Basin Societies (PACIFICHEM 2010)
    • Place of Presentation
      Hawaii, USA
    • Related Report
      2010 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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