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Development of formaldehyde alternative liquid (aqueous N-vinyl-2-pyrolidone) for organ preservation and tissue fixation

Research Project

Project/Area Number 22590177
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General anatomy (including Histology/Embryology)
Research InstitutionOita University

Principal Investigator

FUJIKURA Yoshihisa  大分大学, 医学部, 教授 (10165368)

Co-Investigator(Kenkyū-buntansha) KITAMURA Hirokazu  大分大学, 医学部, 助教 (70115559)
伊奈 啓輔  大分大学, 医学部, 准教授 (20203193)
Co-Investigator(Renkei-kenkyūsha) INA Keisuke  大分大学, 医学部, 准教授 (20203193)
Project Period (FY) 2010-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2013: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywordsホルムアルデヒド代替液 / 親水性高分子モノマー / ピロリドン / 組織固定 / 臓器保存 / 有害化学物質 / 環境対応 / 病原微生物不活性化 / NVP / プリザーブ / ホルマリン代替液 / 病原微生物不活化 / 外科修練 / 解剖実習 / 解剖学 / 残留モノマー定量
Research Abstract

Formaldehyde (FA) was authenticated international cancer carcinogens by IARC in 2007. As an FA is still commonly used in the medical area, we tried to develop FA alternative solutions in this study. Its principal use is in three areas. We examined whether the alternative solutions were effective or not instead of formaldehyde. (1) As a tissue fixative for tissue sections preparation, (2) As a solution of organs over the long term as a gross specimens and storing at, (3) For the embalming and fixation of the donated cadavers. As a result, we got 2 patents, and are commercializing the solution as a name of Preserve.

Report

(5 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (18 results)

All 2014 2013 2012 2011 2010 Other

All Journal Article (7 results) Presentation (9 results) Book (1 results) Patent(Industrial Property Rights) (1 results) (of which Overseas: 1 results)

  • [Journal Article] Administration of antisense DNA for GPR39-1b causes anxiolytic-like responses and appetite loss in rats2012

    • Author(s)
      Yoshinobu Ishitobi, Jotaro Akiyoshi, Shuhei Honda, Taiga Ninomiya, Masayuki Kanehisa, Yoshihiro Tanaka, Jusen Tsuru, Koichi Isogawa, Hirokazu Kitamura, Yoshihisa Fujikura
    • Journal Title

      Neuroscience Research

      Volume: 72(3) Pages: 257-262

    • Related Report
      2013 Final Research Report
  • [Journal Article] Significance ofαSMA in myofibroblasts emerging in tubulointerstitial fibrosis in diabetic nephropathy2011

    • Author(s)
      Keisuke Ina, Hirokazu Kitamura, Shuji Tatsukawa, Yoshihisa Fujikura
    • Journal Title

      Histology and Histopathology

      Volume: 26(7) Pages: 855-866

    • Related Report
      2013 Final Research Report
  • [Journal Article] Infrared radiation has potential antidepressant and anxiolytic effects in animal model of depression and anxiety2011

    • Author(s)
      Yoshihiro Tanaka, Jotaro Akiyoshi, Yoshinari Kawahara, Yoshinobu Ishitobi, Koji Hatano, Nobuhiko Hoaki, Ayumi Mori, Shinjiro Goto, Jusen Tsuru, Hirotaka Matsushita, Hiroaki Hanada, Kensuke Kodama, Koichi Isogawa, Hirokazu Kitamura, Yoshihisa Fujikura
    • Journal Title

      Brain Stimulation

      Volume: 4(2) Pages: 71-76

    • Related Report
      2013 Final Research Report
  • [Journal Article] Human skeletal muscles replaced to a high degree by white adipose tissue2011

    • Author(s)
      Keisuke Ina, Hirokazu Kitamura, Takayuki Masaki, Shuji Tatsukawa, Hironobu Yoshimatsu, Yoshihisa Fujikura
    • Journal Title

      Okajimas Folia Anatomica Japonica

      Volume: 87(4) Pages: 165-170

    • NAID

      130004495086

    • Related Report
      2013 Final Research Report
  • [Journal Article] Epiplakin accelerates the lateral organization of keratin filaments during wound healing2010

    • Author(s)
      Kazushi Ishikawa,Hideaki Sumiyoshi,Noritaka Matsuo, Naoko Takeo, Mizuki Goto, Osamu Okamoto, Shuji Tatsukawa, Hirokazu Kitamura, Yoshihisa Fujikura, Hidekatsu Yoshioka, Sakuhei Fujiwara
    • Journal Title

      Journal of Dermatological Science

      Volume: 60 Pages: 95-104

    • NAID

      10027473970

    • Related Report
      2013 Final Research Report
  • [Journal Article] Evaluation by multidetector row computed tomography2010

    • Author(s)
      Hidetoshi Miyake, Hiro Kiyosue, Shuichi Tanoue, Yoshimi Goto, Hiromu Mori, Yoshihisa Fujikura
    • Journal Title

      Clinical Anatomy

      Volume: 23 Pages: 662-672

    • Related Report
      2013 Final Research Report
  • [Journal Article] Direct inhibition of arginase attenuated allergic and inflame mation in a Dermatophagoides farina-Induced NC/Nga mouse model2010

    • Author(s)
      Noriko Takahashi, Keiki Ogino, Kei Takemoto, Seiji Hamanishi, Da-Hong Wang, Tomoko Takigawa, Masafumi Shibamori, Hironobu Ishiyama, Yoshihisa Fujikura
    • Journal Title

      Am. J. Physiol. Lung Cell Mol. Physiol.

      Volume: 299

    • Related Report
      2013 Final Research Report
  • [Presentation] ホルマリン代替液(N-Vinyl-2-pyrrolidone)注入固定遺体の解剖所見2014

    • Author(s)
      灰塚嘉典、松村讓兒、高篠智、藤倉義久
    • Organizer
      第119回日本解剖学会総会・全国学術集会
    • Place of Presentation
      自治医科大学
    • Year and Date
      2014-03-27
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] サージカルトレーニングに適した固定法の検討-Thiel法およびプリザー®の固定法の試み-2014

    • Author(s)
      河田晋一、小山耕一、林省吾、内藤宗和、曲寧、畑山直之、藤倉義久、伊藤正裕
    • Organizer
      第119回日本解剖学会総会・全国学術集会
    • Place of Presentation
      自治医科大学
    • Year and Date
      2014-03-27
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] ホルムアルデヒド代替液(プリザーブ)を用いた献体者の固定、保存、解剖所見2013

    • Author(s)
      藤倉義久、松村譲兒、雉鼻一郎、松川詠梅、立川修二、北村裕和、伊奈啓輔
    • Organizer
      第11回日本予防医学会学術集会
    • Place of Presentation
      日本科学未来館
    • Year and Date
      2013-06-22
    • Related Report
      2013 Final Research Report
  • [Presentation] ホルマリン代替液の応用-外科修練に向けての基礎的研究-2011

    • Author(s)
      白石恵子, 藤倉義久, 立川修二, 北村裕和, 伊奈啓輔
    • Organizer
      第9回日本予防医学会学術総会
    • Place of Presentation
      首都大学東京荒川キャンパス
    • Year and Date
      2011-11-20
    • Related Report
      2011 Annual Research Report
  • [Presentation] ホルマリン代替液の応用―外科修練に向けての基礎的研究―2011

    • Author(s)
      白石恵子, 藤倉義久, 立川修二, 北村裕和, 伊奈啓輔
    • Organizer
      第9回日本予防医学会学術総会
    • Place of Presentation
      首都大学東京荒川キャンパス
    • Year and Date
      2011-11-19
    • Related Report
      2013 Final Research Report
  • [Presentation] 外科系医療技術修練の為の遺体固定保存方法の基礎的研究2011

    • Author(s)
      藤倉義久、北村裕和、白石恵子、立川修二、伊奈啓輔
    • Organizer
      第116回日本解剖学会総会・全国学術集会
    • Place of Presentation
      横浜(震災影響の為、学術集会中止→誌上開催)
    • Year and Date
      2011-03-29
    • Related Report
      2010 Annual Research Report
  • [Presentation] 外科系医療技術修練の為の遺体固定保存方法の基礎的研究2011

    • Author(s)
      藤倉義久、北村裕和、白石恵子、立川修二、伊奈啓輔
    • Organizer
      第116回日本解剖学会総会・全国学術集会
    • Place of Presentation
      パシフィコ横浜(東日本大震災のため学術集会中止→誌上開催)
    • Related Report
      2013 Final Research Report
  • [Presentation] ホルムアルデヒド代替液(プリザーブ)を用いた献体者の固定、保存、解剖所見

    • Author(s)
      藤倉義久、松村譲兒、雉鼻一郎、松川詠梅、立川修二、北村裕和、伊奈啓輔
    • Organizer
      第11回日本予防医学会学術集会
    • Place of Presentation
      日本科学未来館
    • Related Report
      2013 Annual Research Report
  • [Presentation] ホルマリン代替液固定遺体の解剖

    • Author(s)
      灰塚嘉典、松村讓兒、藤倉義久
    • Organizer
      第101回日本解剖学会関東支部会
    • Place of Presentation
      昭和大学
    • Related Report
      2013 Annual Research Report
  • [Book] The Contribution of Fibronectin ED-A Expression to Myofibroblast Transdifferentiation in Diabetic Renal Fibrosis. Diabetic Nephropathy. ed.by John S.D.Chan2012

    • Author(s)
      Keisuke Ina, Hirokazu Kitamura,Shuji Tatsukawa, Yoshihisa Fujikura
    • Publisher
      In-Tech
    • Related Report
      2013 Final Research Report
  • [Patent(Industrial Property Rights)] 死体保存用注入液2013

    • Inventor(s)
      藤倉義久、雉鼻一郎、松村讓兒
    • Industrial Property Rights Holder
      国立大学法人大分大学、株式会社 日本医化器械製作所、学校法人杏林学園
    • Industrial Property Rights Type
      特許
    • Filing Date
      2013-10-31
    • Related Report
      2013 Final Research Report
    • Overseas

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Published: 2010-08-23   Modified: 2019-07-29  

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