Mechanisms for development and differentiation of cardiomyocytes

• Project/Area Number: 22590183
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General anatomy (including Histology/Embryology)
• Research Institution: Hiroshima University (2012); National Institute of Genetics (2010-2011)
• Principal Investigator: KOKUBO Hiroki 広島大学, 大学院・医歯薬保健学研究院, 講師 (10270480)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 心筋

Research Abstract

In this research, we tried to identify the novel cell population, contributing to the left ventricle, by using genetically engineered mouse of the gene regulating Wnt signaling. As a result, we found a new undifferentiated domain, which contributes to the outflow tract, the atrium in addition to the left ventricle during heart development. This result suggests a new heart formation model: "the heart allows cell migration from at least two undifferentiated cell groups, which contribute the all heart region except for the right ventricle or except for the left ventricle.

Research Products

• [Journal Article] Hesr2 knockout mice develop aortic valve disease with advancing age (2013)
  • Author(s): Kokubo H, Miyagawa-Tomita S, Nakashima Y, Kume T, Yoshizumi M, Nakanishi T,Saga Y
  • Journal Title: Arterioscler.Thromb. Vasc. Biol Volume: 33
• [Journal Article] Hesr2 knockout mice develop aortic valve disease with advancing age (2013)
  • Author(s): Kokubo H, Miyagawa-Tomita S, Nakashima Y, Kume T, Yoshizumi M, Nakanishi T, Saga Y.
  • Journal Title: Arterioscler Thromb Vascular Biology Volume: 33 Issue: 3 Pages: 84-92
  • DOI: 10.1161/atvbaha.112.300573
  • Peer Reviewed
• [Journal Article] Ectopic retinoic acid signaling affects outflow tract cushion development through suppression of the myocardial Tbx2-Tgfβ2 pathway (2012)
  • Author(s): Sakabe M, Kokubo H, Nakajima Y, Saga Y
  • Journal Title: Development Volume: 139 Pages: 385-95
• [Journal Article] Mastermind-like 1 (MamL1) and mastermind-like 3 (MamL3) are essential for Notch signaling in vivo. (2011)
  • Author(s): Oyama T, Harigaya K, Sasaki N, OkamuraY, Kokubo H, Saga Y, Hozumi K, Suganami A, Tamura Y, Nagase T, Koga H, Nishimura M, Sakamoto R, Sato M, Yoshida N, Kitagawa M.
  • Journal Title: Development Volume: 138 Pages: 5235-46
• [Journal Article] Hesr1 and Hesr3 are essential to generate undifferentiated quiescent satellite cells and to maintain satellite cellnumbers (2011)
  • Author(s): Fukada S, Yamaguchi M, Kokubo H, Ogawa R, Uezumi A, Yoneda T, Matev MM, Motohashi N, Ito T, Zolkiewska A, Johnson RL, Saga Y, Miyagoe-Suzuki Y, Tsujikawa K,Takeda S, Yamamoto H.
  • Journal Title: Development. Volume: 138 Pages: 4609-19
• [Journal Article] (MamL1)/Mastermind-like 3 (MamL3) are essential for Notch signaling in vivo (2011)
  • Author(s): Oyama T, Harigaya K, Sasaki N, Okamura Y, Kokubo H, Saga Y, Hozumi K, Suganami A, Tamura Y, Nagase T, Koga H, Nishimura M, Sakamoto R, Sato M, Yoshida N, Kitagawa M
  • Journal Title: Mastermind-like1 Volume: 138 Issue: 23 Pages: 5235-5246
  • DOI: 10.1242/dev.062802
  • Peer Reviewed
• [Journal Article] Hesr1 and Hesr3 are essential to generate undifferentiated quiescent satellite cells and to maintain satellite cell numbers (2011)
  • Author(s): F ukada S, Yamaguchi M, Kokubo H, Ogawa R, Uezumi A, Yoneda T, Matev MM, Motohashi N, Ito T, Zolkiewska A, Johnson RL, Saga Y, Miyagoe-Suzuki Y, Tsujikawa K, Takeda S, Yamamoto H
  • Journal Title: Development Volume: 138 Issue: 21 Pages: 4609-4619
  • DOI: 10.1242/dev.067165
  • Peer Reviewed
• [Journal Article] Endothelin type-A receptor expression defines a distinct subpopulation withinthe first heart field contributing to chamber myocardium (2010)
  • Author(s): Asai R, Kurihara Y, Fujisawa K, Sato T, Kawamura Y, Kokubo H, Tonami K, Nishiyama K, Uchijima Y, Miyagawa-Tomita S, Kurihara H
  • Journal Title: Development Volume: 137 Pages: 3823-3833
• [Journal Article] Endothelin type-A receptor expression defines a distinct subpopulation within the first heart field contributing to chamber myocardium (2010)
  • Author(s): Asai, R., Kokubo, H., et al
  • Journal Title: Development Volume: 137 Pages: 3823-3833
  • Peer Reviewed
• [Presentation] 先天性心疾患から成人性心疾患まで (2012)
  • Author(s): 小久保 博樹
  • Organizer: 心臓発生研究会(日本小児心臓学会)
  • Place of Presentation: 福島
  • Year and Date: 2012-10-19
• [Presentation] 先天性心疾患から成人性心疾患まで (2012)
  • Author(s): 小久保博樹
  • Organizer: 心臓発生研究会（日本小児心臓学会）
  • Place of Presentation: 福島
  • Invited
• [Presentation] 静脈洞から左心室に寄与する新たな細胞系譜 (2011)
  • Author(s): 小久保 博樹, 坂口あかね、 相賀裕美子
  • Organizer: 小児循環器学会
  • Place of Presentation: 福島
  • Year and Date: 2011-10-08
• [Presentation] 静脈洞から左心室に寄与する新たな細胞系譜 (2011)
  • Author(s): 小久保博樹
  • Organizer: 小児循環器学会(心臓血管発生研究会)
  • Place of Presentation: 福島
  • Year and Date: 2011-10-08
• [Presentation] Endothelin type-A receptor expression defines a distinct subpopulation within the first heart field contributing to chamber myocardium (2010)
  • Author(s): Asai, R. Kokubo, H. et al
  • Organizer: 日本分子生物・生化学合同学会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-10
• [Presentation] T-box transcription factor Tbx2 is a key factor in the pathogenesis of congenital heart disease including transposition of great arteries (2010)
  • Author(s): Sakabe, M., Kokubo H. et al
  • Organizer: 日本分子生物・生化学合同学会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-10