Gene transfer to the chick during later embryonic development using transposon

• Project/Area Number: 22590188
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General anatomy (including Histology/Embryology)
• Research Institution: Niigata University
• Principal Investigator: SATO Noboru 新潟大学, 医歯学系, 教授 (00254756)
• Co-Investigator(Renkei-kenkyūsha): SIBATA Masahiro 新潟大学, 医歯学系, 准教授 (10343253)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: ニワトリ胚 / 遺伝子導入 / トランスポゾン / 発育鶏胚

Research Abstract

This study is planned to clear how transposon-mediated gene transfer does work in the chicken embryo during the later period of development. It is shown that transposon-mediated gene transfer allows efficient delivery of the trangene into the chicken embryo until the later period of development. It is also suggested that the gene expression cassette should be precisely constructed to introduce the transgene into the specific cell and tissue.

Research Products

• [Journal Article] Elucidation of target muscle and detailed development of dorsal motor neurons in chick embryo spinal cord. (2013)
  • Author(s): Kobayashi N, Homma S, Okada T, Masuda T, Sato N, Nishiyama K, Sakuma C, Shimada T, Yaginuma H.
  • Journal Title: J Comp Neurol. Volume: in press Issue: 13 Pages: 2987-3002
  • DOI: 10.1002/cne.23326
  • Peer Reviewed
• [Journal Article] 脊髄運動ニューロンの発生と細胞死:その標的との関連 (2012)
  • Author(s): 佐藤昇、柴田昌弘、安戸方邦、伊藤健二朗
  • Journal Title: 新潟医学雑誌 Volume: 第126巻 Pages: 233-237
  • NAID: 120006749322
  • Peer Reviewed
• [Journal Article] Neurogenin2 expression together with NeuroM regulates GDNF family neurotrophic factor receptor α1 (GFRα1) expression in the embryonicspinal cord (2012)
  • Author(s): Shimada T, Yaginuma H, Sato N,Homma S
  • Journal Title: Dev Biol Volume: 320巻 Pages: 250-63
  • Peer Reviewed
• [Journal Article] 脊髄運動ニューロンの発生と細胞死：その標的との関連 (2012)
  • Author(s): 佐藤 昇、柴田昌弘、安戸方邦、伊藤健二朗
  • Journal Title: 新潟医学雑誌 Volume: 126 Pages: 233-237
  • NAID: 120006749322
• [Journal Article] Neurogenin2 expression together with NeuroM regulates GDNF family neurotrophic factor receptor α1 (GFRα1) expression in the embryonic spinal cord. (2012)
  • Author(s): Shimada T, Yaginuma H, Sato N, Homma S
  • Journal Title: Dev Biol. Volume: 370（2） Pages: 250-63
  • Peer Reviewed
• [Journal Article] Intrasulcal electrocorticography in macaque monkeys with minimally invasive neurosurgicalprotocols (2011)
  • Author(s): Matsuo T, Kawasaki K, Osada T, SawahataH, Suzuki T, Shibata M, Miyakawa N, Nakahara K, Iijima A, Sato N, Kawai K, Saito N, Hasegawa I
  • Journal Title: Front Syst Neurosci Volume: 5 Pages: 34-34
  • DOI: 10.3389/fnsys.2011.00034
  • Peer Reviewed
• [Journal Article] Bilateral, asymmetric anomalies ofthe anterior bellies of digastric muscles (2011)
  • Author(s): Y Yamazaki, M Shibata, T Ushiki, K Isokawa, N Sato
  • Journal Title: Journal of Oral Sci Volume: 53(4)巻 Pages: 523-7
  • Peer Reviewed
• [Journal Article] Bilateral, asymmetric anomalies of the anterior bellies of digastric muscles (2011)
  • Author(s): Y Yamazaki, M Shibata, T Ushiki, K Isokawa, N Sato
  • Journal Title: Journal of Oral Sci Volume: 53(4) Pages: 523-7
  • NAID: 130001381392
  • Peer Reviewed
• [Journal Article] Elucidation of target muscle and detaileddevelopment of dorsal motor neurons in chick embryo spinal cord
  • Author(s): Kobayashi N, Homma S, Okada T, Masuda T, Sato N, Nishiyama K, Sakuma C, Shimada T, Yaginuma H
  • Journal Title: J Comp Neurol Volume: (印刷中)
  • Peer Reviewed
• [Presentation] シナプス形成期運動ニューロンへの遺伝子導入法の検討 (2013)
  • Author(s): 千葉映奈、長島寛、柴田昌弘、佐藤昇
  • Organizer: 第118回日本解剖学会全国学術集会
  • Place of Presentation: 高松市
  • Year and Date: 2013-03-30
• [Presentation] Development of a novel model for polyglutamine pathogenesis using the chicken embryo (2012)
  • Author(s): Sato N, Nakayama H, Shibata M, Yasudo M, Ito K, Uemura O, Okamoto H
  • Organizer: 8th FENS Forum of Neuroscience
  • Place of Presentation: バルセロナ(スペイン)
  • Year and Date: 2012-07-15
• [Presentation] Development of a novel model for polyglutamine pathogenesis using the chicken embryo (2012)
  • Author(s): Sato N, Nakayama H, Shibata M, Yasudo M, Ito K, Uemura O, and Okamoto H
  • Organizer: 8ｔｈ FENS Forum of Neuroscience
  • Place of Presentation: バルセロナ（スペイン）
• [Presentation] Gene transfer into the chick by using Tol2 transposition for studying the nervous system development (2011)
  • Author(s): M, Shibata, N, Sato
  • Organizer: 8 th IBRO
  • Place of Presentation: イタリアフィレンツェ国際会議場
  • Year and Date: 2011-07-16
• [Presentation] In vivo imaging of the chick using tol2 transposition (2011)
  • Author(s): 柴田昌弘、伊藤健二郎、佐藤昇
  • Organizer: 第116回日本解剖学会全国学術集会
  • Place of Presentation: 横浜市
  • Year and Date: 2011-03-30
• [Presentation] Gene delivery into developing neurons in the chick using Tol2 transposition (2011)
  • Author(s): 斎藤大樹、柴田昌弘、伊藤健二郎、植村修、岡本仁、佐藤昇
  • Organizer: 第116回日本解剖学会全国学術集会
  • Place of Presentation: 横浜市
• [Presentation] Cre-loxPシステムとTol2トランスポゾンを併用したRNA干渉法は、鶏胚を用いた発生学の研究に有用である (2010)
  • Author(s): 柴田昌宏、伊藤健二朗、佐藤昇
  • Organizer: 第33回日本神経科学大会
  • Place of Presentation: 兵庫県神戸コンベンションセンター
  • Year and Date: 2010-09-04