Epigenetic regulation by transceiption factor GATA3

• Project/Area Number: 22590279
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General medical chemistry
• Research Institution: 公益財団法人東京都医学総合研究所 (2012); Tokyo Metropolitan Organization for Medical Research (2010-2011)
• Principal Investigator: MIYATAKE Shoichiro 公益財団法人東京都医学総合研究所, ゲノム医科学研究分野, 副参事研究員 (30239420)
• Co-Investigator(Kenkyū-buntansha): AOKI Kazuhisa 公益財団法人東京都医学総合研究所, 主任研究員 (00280785)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: ゲノム医化学 / GATA3 / RCOR1 / Zbtb35(ZNF131) / epigenetics / 細胞分化 / 転写因子 / ZNF131 / エピジェネティックス

Research Abstract

Interacting molecules for transcription factor Gata3 were identified and their functions were analyzed. Mouse strain with flag tag integrated Gata3 locus was established. Knockdown of DNA demethylation enzymes in Th2 was performed. Rcor1 and Zbtb35 (Znf131) conditional knockout mice were established. Rcor1 plays important roles in Treg induction. Zbtb35 collaborates with Gata3 during early T lineage development, however in late T lineage development, Zbtb35 has different functions from those of Gata3.

Research Products

• [Journal Article] Interferon regulatory factor-2 regulates hematopoietic stem cells in mouse bone marrow (2012)
  • Author(s): Masumi, A., Miyatake, S., Kohno, T. and Matsuyama, T
  • Journal Title: In Advances in Hematopoietic Stem Cell Research (ed. Pelayo, R.) Volume: (InTech) Pages: 91-112
  • Peer Reviewed
• [Journal Article] The enhancer HS2 critically regulates GATA -3-mediated Il4 transcription in T(H)2 cells (2011)
  • Author(s): Tanaka, S., Motomura, Y., Suzuki, Y., Yagi, R., Inoue, H.,Miyatake, S., and Kubo M
  • Journal Title: Nat Immunol Volume: 12 Pages: 77-85
  • Peer Reviewed
• [Journal Article] The enhancer HS2 critically regulates GATA-3-mediated Il4 transcription in T(H)2 cells. (2011)
  • Author(s): Tanaka, S., Motomura, Y., Suzuki, Y., Yagi, R., Inoue, H., Miyatake, S., Kubo M.
  • Journal Title: Nature Immunology Volume: 12 Pages: 77-85
  • Peer Reviewed
• [Journal Article] Selective down-regulation of Th2 cytokines by C-terminal binding protein 2 in human T cells (2010)
  • Author(s): Kitamura, F., Kitamura, N., Mori, A., Tatsumi, H., Nemoto, S., Miyoshi, H., Miyatake, S., Hiroi, T. and Kaminuma, O
  • Journal Title: Int. Arch. Aller gy Immunol Volume: 152(Suppl 1) Pages: 18-21
  • Peer Reviewed
• [Journal Article] Selective down-regulation of Th2 cytokines by C-terminal binding protein 2 in human T cells. (2010)
  • Author(s): Kitamura, F., Kitamura, N., Mori, A., Tatsumi, H., Nemoto, S., Miyoshi, H., Miyatake, S., Hiroi, T., Kaminuma, O.
  • Journal Title: International Archives of Allergy and Immunology Volume: 152 Pages: 18-21
  • Peer Reviewed
• [Presentation] BTB-ZF transcription factor ZNF131 is crucial for early T cell development in thymus and exerts its function through the interaction with GATA3 KTCC 2013 (2013)
  • Author(s): Miyatake S
  • Organizer: Sixth International Workshop of Kyoto T Cell Conference 2013 (招待講演)
  • Place of Presentation: Kyoto University Shiran Kaikan, Kyoto
• [Presentation] BTB-ZF transcription factor ZNF131 is crucial for early T cell development in thymus and exerts its function through the interaction with GATA3 (2013)
  • Author(s): Miyatake S
  • Organizer: KTCC 2013: Sixth International Workshop of Kyoto T Cell Conference
  • Place of Presentation: Kyoto University Shiran Kaikan
  • Invited
• [Presentation] T細胞の分化に関与するDNA脱メチル化酵素の探索 (2012)
  • Author(s): 青木和久、宮武昌一郎
  • Organizer: 日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場、福岡
• [Presentation] The role of POZ-ZF protein Znf131 in T cell homeostasis Annual Meeting of the Japanese Society for Immunology (2012)
  • Author(s): Iguchi T, Aoki K, Aihara Y and Miyatake S
  • Place of Presentation: Kobe International Conference Center, Kobe
• [Presentation] T 細胞恒常性維持におけるZNF131 の役割 (2012)
  • Author(s): 井口智弘、青木和久、相原祐子、宮武昌一郎
  • Organizer: 第22回 Kyoto T Cell Conference(KTCC)
  • Place of Presentation: 京都
• [Presentation] POZ-ZF protein, ZNF131 is required for the development of T cells during the double negative stage as well as for the maintenance in the periphery Gene Expression & Signaling in the Immune System (2012)
  • Author(s): Iguchi T, Aoki K, Taya C, Sakimura K, and Miyatake S
  • Organizer: Cold Spring Harbor Meeting
  • Place of Presentation: Cold Spring Harbor Institute, New York, USA
• [Presentation] POZ-ZF protein, ZNF131 is required for the development of T cells during the double negative stage as well as for the maintenance in the periphery (2012)
  • Author(s): Iguchi T(Miyatake S)
  • Organizer: Gene Expression & Signaling in the Immune System (Cold Spring Harbor Meeting)
  • Place of Presentation: Cold Spring Harbor Institute, New York, USA
• [Presentation] T細胞恒常性維持におけるZNF131の役割 (2012)
  • Author(s): 井口 智弘（宮武 昌一郎）
  • Organizer: 第22回 Kyoto T Cell Conference (KTCC)
  • Place of Presentation: 和順会館、京都
• [Presentation] The role of POZ-ZF protein Znf131 in T cell homeostasis (2012)
  • Author(s): Iguchi T (Miyatake S)
  • Organizer: Annual Meeting of the Japanese Society for Immunology
  • Place of Presentation: Kobe International Conference Center, Kobe
• [Presentation] Epigenetic regulation in T cell development Annual Meeting of the Molecular Biology Society of Japan (2011)
  • Author(s): Kazuhisa Aoki, Shoichiro Miyatake
  • Place of Presentation: Yokohama
• [Presentation] POZ-ZF タンパク質であるZNF131 のT 細胞分化およびホメオスタシスにおける役割 (2011)
  • Author(s): 井口智弘、宮武昌一郎
  • Organizer: 日本免疫学会総会
  • Place of Presentation: 千葉
• [Presentation] T 細胞分化および活性化における、BTB/POZ ドメインとZn フィンガーを持つ遺伝子 (2011)
  • Author(s): 井口智弘、宮武昌一郎
  • Organizer: ZNF131 の機能解明KTCC(Kyoto T Cell Conference)
  • Place of Presentation: 京都
• [Presentation] T細胞分化および活性化における、BTB/POZドメインとZnフィンガーを持つ遺伝子、ZNF131の機能解明 (2011)
  • Author(s): 井口智弘、宮武昌一郎
  • Organizer: KTCC (Kyoto T Cell Conference)
  • Place of Presentation: 京都
• [Presentation] POZ-ZFタンパク質であるZNF131のT細胞分化およびホメオスタシスにおける役割 (2011)
  • Author(s): 井口智弘、宮武昌一郎
  • Organizer: 日本免疫学会総会
  • Place of Presentation: 千葉
• [Presentation] Epigenetic regulation in T cell development (2011)
  • Author(s): Kazuhisa Aoki, Shoichiro Miyatake
  • Organizer: Annual Meeting of the Molecular Biology Society of Japan
  • Place of Presentation: Yokohama
• [Presentation] T 細胞分化に伴うサイトカイン産生のエピジェネティック制御 (2010)
  • Author(s): 青木和久、宮武昌一郎
  • Organizer: 第33回日本分子生物学会, 第83回日本生化学会 合同大会
  • Place of Presentation: 神戸ポートピアホテル/神戸国際展示場(兵庫県)
• [Presentation] Function of Bst2/tetherin in immune response (2010)
  • Author(s): Iguchi, T. Yoshitani, H. Aoki, K. Miyatake, S
  • Organizer: 14th International Congress of Immunology
  • Place of Presentation: Kobe Portopia Hotel/Kobe International Exhibition Hall, Kobe, Japan
• [Presentation] Il4 Intronic enhancer HS2 is critical element regulating the GATA-3 mediated type 2 helper T cell development (2010)
  • Author(s): Kubo, M., Motomura, Y., Tanaka, S. Suzuki, Y. Inoue, H., Miyatake, S
  • Organizer: 14th International Congress of Immunology
  • Place of Presentation: Kobe Portopia Hotel/Kobe International Exhibition Hall, Kobe, Japan
• [Presentation] GATA3 結合分子群の解析KTCC (2010)
  • Author(s): 宮武昌一郎、青木和久、田沼雄一、村越早絵、井口智弘
  • Organizer: Kyoto T cell Conference
  • Place of Presentation: 京都大学芝蘭会館、京都
• [Presentation] GATA3結合分子群の解析 (2010)
  • Author(s): 宮武昌一郎、青木和久、田沼雄一、村越早絵、井口智弘
  • Organizer: KTCC(Kyoto T cell Conference)
  • Place of Presentation: 京都大学芝蘭会館(京都府)
• [Presentation] Il4 Intronic enhancer HS2 is critical element regulating the GATA-3 mediated type 2 helper T cell development. (2010)
  • Author(s): Kubo, M., Motomura, Y., Tanaka S., Suzuki, Y., Inoue, H., Miyatake, S.
  • Organizer: 14^<th> International Congress of Immunology
  • Place of Presentation: Kobe Portopia Hotel/Kobe International Exhibition Hall, Kobe, Japan
• [Presentation] Function of Bst2/tetherin in immune response. (2010)
  • Author(s): Iguchi, T., Yoshitani, H., Aoki, K., Miyatake, S.
  • Organizer: 14^<th> International Congress of Immunology
  • Place of Presentation: Kobe Portopia Hotel/Kobe International Exhibition Hall, Kobe, Japan
• [Presentation] T細胞分化に伴うサイトカイン産生のエピジェネティック制御 (2010)
  • Author(s): 青木和久、宮武昌一郎
  • Organizer: 第33回日本分子生物学会 第83回日本生化学会合同大会
  • Place of Presentation: 神戸ポートピアホテル/神戸国際展示場(兵庫県)
• [Book] Advances in Hematopoietic Stem Cell Research, Interferon regulatory factor-2 regulates hematopoietic stem cells in mouse bone marrow(R.P.Camacho (ed.)) (2012)
  • Author(s): Masumi, A., Takizawa, K., Mizukami, T., Kuramitsu, M., Momose, H., Miyatake, S., Kohno, T., Matsuyama, T., Yamaguchi, K., Hamaguchi, I.
  • Total Pages: 464
  • Publisher: InTech