Role of Wnt signal modulators in malignancy of cancer

• Project/Area Number: 22590282
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Pathological medical chemistry
• Research Institution: University of Tsukuba
• Principal Investigator: UCHIDA Kazuhiko 筑波大学, 医学医療系, 准教授 (90211078)
• Co-Investigator(Kenkyū-buntansha): SHIMAZUI Toru 筑波大学, 医学医療系, 教授 (80235613)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: Wntシグナル / プロテオミクス / 分子標的薬 / 腎明細胞癌 / 肝細胞がん / 質量分析 / 遺伝子発現解析 / シグナル伝達 / 腎がん / 肝がん / Wnt / siRNA / 浸潤 / 腎明細胞がん

Research Abstract

In this study, we analyzed the mechanisms of Wnt signaling modulator in carcinogenesis and malignant transformation. We have found overexpression of novel Wnt modulator. Membrane Protein X (MPX) by ‘omics’ analysis of liver cancer. Functional analysis of MPX by over-expression of cDNA and siRNA knockdown for MPX revealed this new Wnt signaling modulator promote cancer invasion in liver cancer.

Research Products

• [Journal Article] Serum protein isoform profiles indicate the progression of hepatitis C virus-induced liver diseases. (2013)
  • Author(s): Sugimoto K, Shiraki K, Takei Y, Ito M, Nobori T, Suzuki H, Dissanayaka SK, Meno K, Asashima M, Uchida K
  • Journal Title: Int J Mol Med Volume: Vol. 31 Issue: 6 Pages: 943-950
  • DOI: 10.1111/cas.12149
  • Peer Reviewed
• [Journal Article] Systemic transduction of p16INK4A antitumor peptide inhibits the growth of MBT-2 mouse bladder tumor cell line grafts (2013)
  • Author(s): Shimazui T, Yoshikawa K, Miyazaki J, Kojima T, Inai H, Ando S, Uemura H, Uchida K, Nishiyama H
  • Journal Title: Int. J. Oncol Volume: Vo l .42 Issue: 2 Pages: 543-54
  • DOI: 10.3892/ijo.2012.1752
  • Peer Reviewed
• [Journal Article] Serum protein isoform profiles indicate the progression of hepatitis C virus-induced liver diseases. (2013)
  • Author(s): Sugimoto K, Uchida K et al.
  • Journal Title: Int J Mol Med. Volume: Int J Mol Med. Pages: 943-950
  • Peer Reviewed
• [Journal Article] Delayed cutaneous wound healing in Fam129b/Minerva-deficient mice. (2012)
  • Author(s): Oishi H et al.
  • Journal Title: J. Biochem Volume: 152 Issue: 6 Pages: 549-555
  • DOI: 10.1093/jb/mvs100
  • Peer Reviewed
• [Journal Article] Genome-wide identification of endothelial cell-enriched genes in the mouse embryo. (2012)
  • Author(s): Takase H, Matsumoto K, Yamadera R, Kubota Y, Otsu A, Suzuki R, Ishitobi H, Mochizuki H, Kojima T, Takano S, Uchida K, Takahashi S, Ema M
  • Journal Title: Blood Volume: 120 Pages: 914-914
  • Peer Reviewed
• [Journal Article] Genome-wide identification of endothelial cell-enriched genes in the mouse embryo (2012)
  • Author(s): Takase H, Matsumoto H, Yamadera R, Kubota Y, Otsu A, Suzuki R, Ishitobi H, Mochizuki H, Kojima T, Takano S, Uchida K, Takahashi S, Ema M
  • Journal Title: Blood Volume: 120 Issue: 4 Pages: 914-923
  • DOI: 10.1182/blood-2011-12-398156
  • Peer Reviewed
• [Journal Article] Molecular Biomarker DIscovery in Hepatocellular Carcinoma (2012)
  • Author(s): Uchida, K., Shiraki K
  • Journal Title: Cancers Volume: (In press)
  • Peer Reviewed
• [Journal Article] 網羅的プロテオミクスによる膵癌のバイオマーカーの探索 (2011)
  • Author(s): 白木克哉, 杉本和史, 内田和彦
  • Journal Title: 胆と膵 Volume: Vol. 32 Pages: 811-815
  • Peer Reviewed
• [Journal Article] c-Maf plays a crucial role for the definitive erythropoiesis that accompanies erythroblastic island formation in the fetal liver (2011)
  • Author(s): 日下部学、長谷川和輝、濱田理人、中村恵弥、大住貴之、鈴木宏奈、工藤崇、内田和彦、二宮治彦、千葉滋、高橋智
  • Journal Title: Blood Volume: 118 Issue: 5 Pages: 1374-1385
  • DOI: 10.1182/blood-2010-08-300400
  • Peer Reviewed
• [Journal Article] c-Maf plays a crucial role for the definitive erythropoiesis that accompanies erythroblastic island formation in the fetal liver (2011)
  • Author(s): Kusakabe M, Uchida K., et al
  • Journal Title: Blood Volume: 118
  • Peer Reviewed
• [Journal Article] FOXO1 and TCF7L2 genes involved in metastasis and poor prognosis in clear cell renal cell carcinoma (2011)
  • Author(s): Kojima, T, Uchida, K, et al.
  • Journal Title: Genes Chromosomes Cancer Volume: 49 Pages: 379-389
  • Peer Reviewed
• [Journal Article] FOXO1 and TCF7L2 genes involved in metastasis and poor prognosis in clear cell renal cell carcinoma. Genes Chrom. (2010)
  • Author(s): Kojima T, Shimazui T, Horie R, Hinotsu S, Oikawa T, Kawai K, Suzuki H, Meno K, Akaza H, Uchida K
  • Journal Title: Cancer Volume: Vol.49 Issue: 4 Pages: 379-389
  • DOI: 10.1002/gcc.20750
  • Peer Reviewed
• [Journal Article] Up-regulation of glypican-3 in human hepatocellular carcinoma (2010)
  • Author(s): Suzuki M, Sugimoto K, Tanaka J, Tameda M, Inagaki Y, Kusagawa S, Nojiri K, Beppu T, Yoneda K, Yamamoto N, Ito M, Yoneda M, Uchida K, Takase K, Shiraki K
  • Volume: Vol.30
  • Pages: 5055-61
  • Peer Reviewed
• [Journal Article] Functional cell surface expression of Toll-like receptor 9 promotes cell proliferation and survival in human hepatocellular carcinomas (2010)
  • Author(s): Tanaka J, Sugimoto K, Shiraki K, Tameda M, Kusagawa S, Nojiri K, Beppu T, Yoneda K, Yamamoto N, Uchida K, Kojima T, Takei Y
  • Journal Title: Int J Oncol Volume: 37 Pages: 805-14
  • Peer Reviewed
• [Journal Article] Laser microdissection-based analysis of cytokine balance in the kidneys of patients with lupus nephritis (2010)
  • Author(s): Wang Y, Ito S, Chino Y, Goto D, Matsumoto I, Murata H, Tsutsumi A, Hayashi T, Uchida K, Usui J, Yamagata K, Sumida T.
  • Journal Title: Clin Exp Immunol. Volume: Vol.159 Pages: 1-10
  • Peer Reviewed
• [Journal Article] Laser microdissection-based analysis of cytokine balance in the kidneys of patients with lupus nephriti (2010)
  • Author(s): Wang, Y., Uchida, K., et al.
  • Journal Title: Clin Exp Immunol. Volume: 159 Pages: 1-10
  • Peer Reviewed
• [Journal Article] Up-regulation of glypican-3 in human hepatocellular carcinom (2010)
  • Author(s): Suzuki, M., Uchida, K., et al.
  • Journal Title: Anticancer Res. Volume: 30 Pages: 5955-5061
  • Peer Reviewed
• [Presentation] Peptidomeによるバイオマーカー探索 (2012)
  • Author(s): 内田和彦
  • Organizer: 生命医薬情報学連合大会2012(招待講演)
  • Place of Presentation: 東京
  • Year and Date: 2012-10-14
• [Presentation] Discovery of'cell-derived'circulating peptide biomarker in blood and LC-MS/MS assay developmentfor liver disease. (2012)
  • Author(s): Uchida, K
  • Organizer: AACC2012
  • Place of Presentation: Los Angeles, USA
  • Year and Date: 2012-07-15
• [Presentation] dentification of serum biomarkers for transaminase-negative chronic hepatitis and nonalcoholic fatty liver disease by proteomics (2012)
  • Author(s): Uchida K., Suzuki H, Meno K, Korenaga T, Nagashima R, Sugimoto K, Toyota H, Kumada S, Okanoue T, Shiraki K
  • Organizer: The 21th Asian Pacific Association for the Study of the Liver (APASL 2011) (Plenary lecture)
  • Place of Presentation: Bangkok, Thailand
  • Year and Date: 2012-02-18
• [Presentation] Discovery of‘cell-derived’circulating peptide biomarker in blood and LC-MS/MS assay development for liver disease (2012)
  • Author(s): Uchida K et al.
  • Organizer: AACC2012
  • Place of Presentation: Los Angeles
• [Presentation] 高密度オリゴヌクレオチドアレイによる新規肝癌治療標的分子の探索 (2012)
  • Author(s): 杉本和史、内田和彦ら
  • Organizer: 第48回日本肝臓病学会
  • Place of Presentation: 金沢
• [Presentation] Peptidomeによるバイオマーカー探索 (2012)
  • Author(s): 内田和彦
  • Organizer: 生命医薬情報学連合大会2012
  • Place of Presentation: 東京
  • Invited
• [Presentation] Peptidomic and immunological analyses reveal novel circulating biomarkers for nonalcoholic fatty liver disease (2011)
  • Author(s): Korenaga T, Meno K, Suzuki H, Sugimoto K, Shiraki K, Uchida K
  • Organizer: 第34回日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-13
• [Presentation] Peptidomic and immunological analyses reveal novel circulating biomarkers for nonalcoholic fatty liver diseases (2011)
  • Author(s): Korenaga T., Uchida, K, et al.
  • Organizer: 第34回日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-13
• [Presentation] 認知症の早期診断のための血液バイオマーカー探索 (2011)
  • Author(s): 内田和彦
  • Organizer: オミックス医療研究会シンポジウム・定期講演会
  • Place of Presentation: 東京(招待講演)
  • Year and Date: 2011-07-07
• [Presentation] Pooled Sampleを用いた血清中のNASHバイオマーカーペプチドのiTRAQによる同定 (2011)
  • Author(s): 杉本和史, 山本憲彦, 白木克哉, 竹井謙之, 熊田卓, 内田和彦
  • Organizer: 第47回日本肝臓学会総会
  • Place of Presentation: 東京
  • Year and Date: 2011-06-02
• [Presentation] 二次元電気泳動と多段階質量分析を利用した新規HCV関連肝疾患進展マーカーの検出 (2011)
  • Author(s): 為田雅彦、杉本和史、白木克哉、山本憲彦、竹井謙之、内田和彦
  • Organizer: 第47回日本肝臓学会総会
  • Place of Presentation: 東京
  • Year and Date: 2011-06-02
• [Presentation] Pooled Sampleを用いた血清中のNASHバイオマーカーペプチドのiTRAQによる同定 (2011)
  • Author(s): 杉本和史、内田和彦, ほか
  • Organizer: 第47回日本肝臓学会総会(ワークショップ)
  • Place of Presentation: 東京
  • Year and Date: 2011-06-02
• [Presentation] 網羅的プロテオミクスによる膵癌のバイオマーカーの探索 (2011)
  • Author(s): 白木克哉、杉本和史、熊田卓、内田和彦
  • Organizer: 第97回日本消化器病学会総会
  • Place of Presentation: 東京
  • Year and Date: 2011-05-15
• [Presentation] New Strategy for Identification and Assay Development of Circulating Peptide Biomarkers for Chronic Liver Disease and Hepatocellular Carcinoma (2010)
  • Author(s): Uchida, K.
  • Organizer: HUPO2010
  • Place of Presentation: Sydney, Australia
  • Year and Date: 2010-09-20
• [Presentation] New Strategy for Identification and Assay Development of Circulating Peptide Biomarkers for Chronic Liver Disease and Hepatocellular Carcinoma. (2010)
  • Author(s): Uchida K, Suzuki, H., Meno, K., Ishii, T., Sugimoto, K., Nagashima, N., Shiraki, K.
  • Organizer: 国際ヒトプロテオーム機構HUPO 9th World Congres.
  • Place of Presentation: Sydney, Australia
  • Year and Date: 2010-09-09
• [Presentation] Quantitative Peptidomic Analysis Reveals Novel Circulating Biomarkers for Chronic Inflammation in Transaminase-Negative Chronic Hepatitis and Nonalcoholic Fatty Liver Disease. (2010)
  • Author(s): Suzuki, H., Meno, K., Korenaga, T., Ishii, T., Sugimoto, K., Nagashima, R., Shiraki, K., Uchida, K
  • Organizer: 国際ヒトプロテオーム機構HUPO 9th World Congres
  • Place of Presentation: Sydney, Australia
  • Year and Date: 2010-09-09
• [Presentation] Dishevelled阻害薬(FJ9)における腎癌細胞株のWntシグナルの抑制と抗腫瘍効果に関する検討 (2010)
  • Author(s): 小島崇宏, 島居徹, 及川剛宏, 河合弘二, 内田和彦, 赤座英之
  • Organizer: 第98回日本泌尿器科学会総会
  • Place of Presentation: 盛岡
  • Year and Date: 2010-04-27
• [Book] Renal Cell Carcinoma(In press) (2011)
  • Author(s): Uchida, K.
  • Publisher: InTech-Open Access Publisher