The implication of conformational change of ST2 gene product due to polymorphism for various diseases.

• Project/Area Number: 22590289
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Pathological medical chemistry
• Research Institution: Jichi Medical University
• Principal Investigator: TOMINAGA Shinichi 自治医科大学, 医学部, 教授 (70155571)
• Co-Investigator(Kenkyū-buntansha): TAMEMOTO Hiroyuki 自治医科大学, 医学部, 准教授 (90292630) ; KOMINE Mayumi 自治医科大学, 医学部, 准教授 (00282632) ; HAYAKAWA Morisada 自治医科大学, 医学部, 講師 (30326847)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: ST2遺伝子 / IL-33 / シグナル伝達 / ST2 / タンパク質 / 免疫学 / アレルギー疾患 / リガンド

Research Abstract

Effect of novel polymorphism on human ST2 gene was studied. Comparison of signal transduction of IL-33 in stable transformants expressing A78 type ST2L and E78 type ST2L resulted in no remarkable difference. Measurement using surface plasmon resonance met

Research Products

• [Journal Article] Presence of a novel exon 2E encoding a putative transmembrane protein in human IL-33 gene (2013)
  • Author(s): Tominaga, S., Hayakawa, M., Tsuda, H., Ohta, S., and Yanagisawa, K
  • Journal Title: Biochem. Biophys. Res. Commun Volume: 430 Issue: 3 Pages: 969-974
  • DOI: 10.1016/j.bbrc.2012.12.050
  • Peer Reviewed
• [Journal Article] YM-175 induces apoptosis of human native monocyte-lineage cells via inhibition of prenylation. (2012)
  • Author(s): Miwa, A., Takezako, N., Hayakawa, H., Hayakawa, M., Tominaga, S., and Yanagisawa, K.
  • Journal Title: Am. J. Hematol Volume: 87 Issue: 12 Pages: 1084-1088
  • DOI: 10.1002/ajh.23328
  • Peer Reviewed
• [Journal Article] Novel splice variants of IL-33: Differential expression in normal and transformed cells (2012)
  • Author(s): Tsuda, H., Komine, M., Karakawa, M., Etoh, T., Tominaga, S., and Ohtsuki, M
  • Journal Title: J. Invest. Dermatol Volume: 132 Issue: 11 Pages: 2661-2664
  • DOI: 10.1038/jid.2012.180
  • Peer Reviewed
• [Journal Article] Soluble ST2 protein inhibits LPS stimulation on monocyte-derived dendritic cells (2012)
  • Author(s): Nagata, A., Takezako, N., Tamemoto, H., Ohto-Ozaki, H., Ohta, S., Tominaga, S., and Yanagisawa, K.
  • Journal Title: Cell. Mol. Immunol Volume: 9 Issue: 5 Pages: 399-409
  • DOI: 10.1038/cmi.2012.29
  • Peer Reviewed
• [Journal Article] Regulation of IL-33 expression by IFN-γ and tumor necrosis factor-α in normal human epidermal keratinocytes. (2012)
  • Author(s): Meephansan, J., Tsuda, H., Komine, M., Tominaga, S., and Ohtsuki, M
  • Journal Title: J. Invest. Dermatol Volume: 132 Issue: 11 Pages: 2593-2600
  • DOI: 10.1038/jid.2012.185
  • Peer Reviewed
• [Journal Article] The levels of soluble ST2 in sera and bullous fluid from patients with bullous pemphigoid. (2012)
  • Author(s): Wakatabi, K., Komine, M., Meephansan, J., Matsuyama, Y., Tsuda, H., Tominaga, S., and Ohtsuki, M.
  • Journal Title: Eur. J. Dermatol Volume: 22 Issue: 3 Pages: 333-336
  • DOI: 10.1684/ejd.2012.1706
  • Peer Reviewed
• [Journal Article] Ultraviolet B irradiation induces the expression of IL-33 mRNA and protein in normal human epidermal keratinocytes (2012)
  • Author(s): Meephansan, J., Komine, M., Tsuda, H., Tominaga, S., and Ohtsuki, M.
  • Journal Title: J. Dermatol. Sci Volume: 65 Issue: 1 Pages: 72-74
  • DOI: 10.1016/j.jdermsci.2011.10.004
  • Peer Reviewed
• [Journal Article] Sustained elevation of interleukin-33 in sera and synovial fluids from patients with rheumatoid arthritis non-responsive to anti-tumor necrosis factor: possible association with persistent IL-1βsignaling and a poor clinical response (2012)
  • Author(s): Matsuyama, Y., Okazaki, H., Hoshino, M., Onishi, S., Kamata, Y., Nagatani, K., Nagashima, T., Iwamoto, M., Yoshio, T., Ohto-Ozaki, H., Tamemoto, H., Komine, M.,Sekiya, H., Tominaga, S. and Minota, S.
  • Journal Title: Rheumatol. Int Volume: 32 Issue: 5 Pages: 1397-1401
  • DOI: 10.1007/s00296-011-1854-6
  • Peer Reviewed
• [Journal Article] JAK2 is an important signal transducer in IL-33-induced NF-κB activation. (2011)
  • Author(s): Funakoshi-Tago, M., Tago, K., Sato, Y., Tominaga, S., and Kasahara, T.
  • Journal Title: Cell. Signal Volume: 23 Issue: 2 Pages: 363-370
  • DOI: 10.1016/j.cellsig.2010.10.006
  • Peer Reviewed
• [Journal Article] JAK2 is an important signal transducer in IL-33-induced NF-κB activation. (2011)
  • Author(s): Funakoshi-Tago, M
  • Journal Title: Cell.Sigunal. Volume: VOL.23 Pages: 363-370
  • Peer Reviewed
• [Journal Article] Characterization of ST2 transgenic mice with resistance to IL-33. (2010)
  • Author(s): Ohto-Ozaki, H., Kuroiwa, K., Mato, N., Matsuyama, Y., Hayakawa, M., Tamemoto, H., and Tominaga, S.
  • Journal Title: Eur. J. Immunol Volume: 40 Issue: 9 Pages: 2632-2642
  • DOI: 10.1002/eji.200940291
  • Peer Reviewed
• [Journal Article] Characterization of ST2 transgenic mice with resistance IL-33. (2010)
  • Author(s): Ohto-Ozaki, H
  • Journal Title: Eur.J.Immunol. Volume: VOL.40 Pages: 2632-2642
  • Peer Reviewed
• [Journal Article] IL-33の基礎とアレルギー性炎症の誘導 (2010)
  • Author(s): 富永真一
  • Journal Title: 炎症と免疫 Volume: VOL.18 Pages: 15-20
• [Presentation] ST2遺伝子の選択的スプライシングにおけるトランス因子の解析 (2012)
  • Author(s): 鴨下信彦、為本浩至、早川盛禎、富永眞一
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡
  • Year and Date: 2012-12-15
• [Presentation] Soluble ST2 protein inhibits LPS stimulation on monocyte-derived dendritic cells (2012)
  • Author(s): Nagata, A., Ohta, S., Tominaga, S.,Yanagisawa, K
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
  • Year and Date: 2012-12-14
• [Presentation] Shedding of receptor for IL-33 (ST2L) (2012)
  • Author(s): Tamemoto, H., Kamoshita, N., Kashiwada, M., Hayakawa, M., Ozaki, H., Tominaga, S
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
  • Year and Date: 2012-12-12
• [Presentation] Nuclear IL-33 in relation to proliferation and cell cycle of normal human epidermal keratinocytes (2012)
  • Author(s): Tsuda, H., Komine, M., Oshio, T., Tominaga, S., Ohtsuki, M
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
  • Year and Date: 2012-12-11
• [Presentation] IL-17 up regulates IL-33 expression in keratinocytes; negative feedback loop in psoriatic epidermis (2012)
  • Author(s): Meephansan, J., Tsuda, H., Komine, M., Karakawa, M., Tominaga, S., Ohtsuki, M
  • Organizer: The 42ndAnnual ESDR (European Society for Dermatological Research)Meeting
  • Place of Presentation: Venice, Italy
• [Presentation] Regulation of IL-33 expression by interferon-gamma and tumor necrosis factor-alpha in normal human epidermal keratinocytes. (2012)
  • Author(s): Meephansan, J., Tsuda, H., Komine, M., Karakawa, M., Tominaga, S., Ohtsuki, M
  • Organizer: Annual Meeting of the Society for Investigative Dermatology
  • Place of Presentation: Raleigh, North Carolina, USA
• [Presentation] Regulation of IL-33 expression by interferon-gamma and tumor necrosis factor-alpha in normal human epidermal keratinocytes. (2012)
  • Author(s): Meephansan, J., Tsuda, H., Komine, M., Karakawa, M., Tominaga, S., Ohtsuki, M.
  • Organizer: Annual Meeting of the Society for Investigative Dermatology.
  • Place of Presentation: Raleigh, North Carolina, USA
• [Presentation] IL-17 up regulates IL-33 expression in keratinocytes; negative feedback loop in psoriatic epidermis. (2012)
  • Author(s): Meephansan, J., Tsuda, H., Komine, M., Karakawa, M., Tominaga, S., Ohtsuki, M.
  • Organizer: The 42nd Annual ESDR (European Society for Dermatological Research) Meeting.
  • Place of Presentation: Venice, Italy
• [Presentation] IL-33は核内でケラチノサイトの細胞増殖に関与している。 (2012)
  • Author(s): Tsuda, H., Komine, M., Oshio, T., Tominaga, S., Ohtsuki, M.
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
• [Presentation] IL-33受容体ST2Lの細胞外ドメイン放出。 (2012)
  • Author(s): Tamemoto, H., Kamoshita, N., Kashiwada, M., Hayakawa, M., Ozaki, H., Tominaga, S.
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
• [Presentation] ヒト単球由来樹状細胞に対する分泌型ST2タンパク質の作用。 (2012)
  • Author(s): Nagata, A., Ohta, S., Tominaga, S., Yanagisawa, K.
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
• [Presentation] ST2遺伝子の選択的スプライシングにおけるトランス因子の解析。 (2012)
  • Author(s): 鴨下信彦、為本浩至、早川盛禎、富永眞一
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡
• [Presentation] Alternative splicing in ST2 gene. (2011)
  • Author(s): Kamoshita, N., Tamemoto, H., Hayakawa, M., Tominaga, S
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-16
• [Presentation] Alternative splicing in ST2 gene (2011)
  • Author(s): Kamoshita, N., et al
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2011-12-16
• [Presentation] Novel splicing variants of IL-33 and its expression variation (2011)
  • Author(s): Tsuda, H., Meephansan, J., Karakawa, M., Tominaga, S., Komine, M., and Ohtsuki, M.
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-15
• [Presentation] Novel splicing variants of IL-33 and its expression variation (2011)
  • Author(s): Tsuda, H., et al
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2011-12-15
• [Presentation] Localization of intracellular domain of ST2L. (2011)
  • Author(s): Tamemoto, H., Hayakawa, M., Kamoshita, N., Ozaki, H., Tominaga, S.
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-14
• [Presentation] Localization of intracellular domain of ST2L (2011)
  • Author(s): Tamemoto, H., et al
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2011-12-14
• [Presentation] IL-33 is produced by normal human keratinocytes with environmental and inflammatory stimuli: A novel "alarmin" in the skin (2011)
  • Author(s): Meephansan, J., Komine, M., Tsuda, H., Yano, S., Tominaga, S., Ohtsuki, M
  • Organizer: Annual Meeting of the Society for Investigative Dermatology
  • Place of Presentation: Phoenix, Arizona, USA
• [Presentation] Sustained elevation of interleukin-33 in serum and synovial fluid is associated with a poor response of rheumatoid arthritis to anti-tumor necrosis factor therapy (2010)
  • Author(s): Matsuyama, Y., Okazaki, H., Hoshino, T., Onishi, S., Ohto-Ozaki, H., Tamemoto, H., Tominaga, S., and Minota, S.
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会 合同年会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-09
• [Presentation] Sustained elevation of interleukin-33 in serum and synovial fluid is associated with a poor response of rheumatoid arthritis to anti-tumor necrosis factor therapy. (2010)
  • Author(s): Matsuyama, Y
  • Organizer: 日本分子生物学会・日本生化学会 合同年会
  • Place of Presentation: 神戸国際会議場(兵庫県)
  • Year and Date: 2010-12-09
• [Presentation] Soluble ST2 attenuated the IL-33-induced M2 polarization of peritoneal macrophages (2010)
  • Author(s): Ohto-Ozaki, H., Kuroiwa, K., Matsuyama, Y., Hayakawa, M., Tamemoto, H., and Tominaga, S.
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会 合同年会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-08
• [Presentation] Ectodomain shedding of ST2L, a member of IL-1 receptor family. (2010)
  • Author(s): Tamemoto, H., Ozaki, H., Kamoshita, N., Hayakawa, M., and Tominaga, S.
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会 合同年会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-08
• [Presentation] Ectodomain shedding of ST2L, a member of IL-1 receptor family. (2010)
  • Author(s): Tamemoto, H
  • Organizer: 日本分子生物学会・日本生化学会 合同年会
  • Place of Presentation: 神戸国際会議場(兵庫県)
  • Year and Date: 2010-12-08
• [Presentation] Soluble ST2 attenuated the IL-33-induced M2 polarization of peritoneal Macrophages. (2010)
  • Author(s): Ohto-Ozaki, H
  • Organizer: 日本分子生物学会・日本生化学会 合同年会
  • Place of Presentation: 神戸国際会議場(兵庫県)
  • Year and Date: 2010-12-08
• [Presentation] Environmental stimuli up regulate IL-33 in normal human keratinocytes. (2010)
  • Author(s): Meephansan, J., Komine, M., Wakatabi, K., Tominaga, S., Ohtsuki, M.
  • Organizer: Annual Meeting of the Society for Investigative Dermatology
  • Place of Presentation: Atlanta, Georgia, USA
• [Book] IL-33の基礎とアレルギー性炎症の誘導 炎症と免疫 (2010)
  • Author(s): 早川盛禎、富永眞一
  • Publisher: 先端医学社