Different toxic activities of adenylate cyclase toxins of Bordetella pertussisand Bordetella bronchiseptica

• Project/Area Number: 22590390
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Bacteriology (including Mycology)
• Research Institution: Osaka University
• Principal Investigator: KAMITANI Shigeki 大阪大学, 微生物病研究所, 助教 (60379089)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 病原性 / 百日咳 / 細菌毒素 / 宿主特異性 / 百日咳菌

Research Abstract

We found that different toxic activities of adenylate cyclase toxins (ACTs) produced by Bordetella species including B. pertussis, B. parapertussis, and B. bronchisepticacome from the different activities of translocation of ACTs to the target cells. Furthermore, we showed that the different toxic activities among Bordetellaspecies could be a cause of the different pathology of diseases.

Research Products

• [Journal Article] Defining the substrate specificity of Pasteurella multocida toxin-induced deamidation of α-subunits of heterotrimeric G proteins (2013)
  • Author(s): Orth, J. H., Fester, I., Siegert, P., Weise, M., Lanner, U., Kamitani, S., Tachibana, T., Wilson, B. A., Schlosser, A., Horiguchi, Y., and Aktories,K
  • Journal Title: FASEB J Volume: 27 Issue: 2 Pages: 832-842
  • DOI: 10.1096/fj.12-213900
  • Peer Reviewed
• [Journal Article] IFN-γ elicits macrophage autophagy via the p38 MAPK signaling pathway (2012)
  • Author(s): Matsuzawa, T., Kimb B-H., Kamitani, S., Miyake, M., and MacMicking, J. D
  • Journal Title: J Immunol Volume: 189 Issue: 2 Pages: 813-818
  • DOI: 10.4049/jimmunol.1102041
  • Peer Reviewed
• [Journal Article] Differences Between Group X and Group V Secretory Phospholipase A2 in Lipolytic Modification of Lipoproteins (2012)
  • Author(s): Kamitani, S., Yamada, K., Yamamoto, S., Ishimoto, Y., Ono, T., Saiga, A., and Hanasaki, K.
  • Journal Title: Cell Mol Biol Lett Volume: 17 Issue: 3 Pages: 459-479
  • DOI: 10.2478/s11658-012-0019-2
  • Peer Reviewed
• [Journal Article] Enzymatic actions of Pasteurella multocida toxin detected by monoclonal antibody recognizing the deamidated alpha subunit of the heterotrimeric GTPase Gq (2011)
  • Author(s): Kamitani, S., Ao, S., Toshima, H., Tachibana, T., Hashimoto, M., Kitadokoro, K., Fukui-Miyazaki, A., Abe, H., and Horiguchi, Y.
  • Journal Title: FEBS J Volume: 278 Issue: 15 Pages: 2702-2712
  • DOI: 10.1111/j.1742-4658.2011.08197.x
  • Peer Reviewed
• [Journal Article] Crystal Structure of Clostridiumperfringensenterotoxin displays features of β-pore-forming toxins (2011)
  • Author(s): Kitadokoro, K., Nishimura, K., Kamitani, S., Fukui-Miyazaki, A., Toshima, H., Abe, H., Kamata, Y., Sugita-Konishi, Y., Yamamoto, S., Karatani, H., and Horiguchi, Y
  • Journal Title: J Biol Chem Volume: 286 Issue: 22 Pages: 19549-19555
  • DOI: 10.1074/jbc.m111.228478
  • Peer Reviewed
• [Journal Article] Bordetella dermonecrotic toxin binds to target cells via the N-terminal 30 amino acids. (2011)
  • Author(s): Fukui-Miyazaki, A., Ohnishi, S., Kamitani, S., Abe, H., and Horiguchi, Y
  • Journal Title: Microbiol Immunol Volume: 55 Pages: 154-159
  • NAID: 10031121454
  • Peer Reviewed
• [Journal Article] Bordetella Dermonecrotic Toxin Binds to Target Cells Via the N-Terminal 30 Amino Acids (2011)
  • Author(s): Fukui-Miyazaki, A., S.Ohnishi, S.Kamitani, H.Abe, Y.Horiguchi
  • Journal Title: Microbiology and Immunology Volume: 55 Pages: 154-9
  • NAID: 10031121454
  • Peer Reviewed
• [Journal Article] Bordetella dermonecrotic toxin binds to target cells via the N-terminal 30 amino acids. (2011)
  • Author(s): Fukui-Miyazaki A., Kamitani S, et al.
  • Journal Title: Microbiology and Immunology Volume: 55 Pages: 154-159
  • NAID: 10031121454
  • Peer Reviewed
• [Journal Article] Association of Bordetella dermonecrotic toxin with the extracellular matrix (2010)
  • Author(s): Fukui-Miyazaki, A., Kamitani, S., Miyake,M., and Horiguchi, Y
  • Journal Title: BMC microbiology Volume: 10 Issue: 1 Pages: 247-247
  • DOI: 10.1186/1471-2180-10-247
  • Peer Reviewed
• [Journal Article] Characterization of the membrane-targeting C1 domain in Pasteurella multocidatoxin (2010)
  • Author(s): Kamitani, S., Kitadokoro, K, Miyazawa,M., Toshima, H., Fukui, A., Abe, H., Miyake,M., and Horiguchi, Y.
  • Journal Title: J Biol Chem Volume: 285 Issue: 33 Pages: 25467-25475
  • DOI: 10.1074/jbc.m110.102285
  • Peer Reviewed
• [Journal Article] Characterization of the membrane-targeting C1 domain in Pasteurella multocida toxin. (2010)
  • Author(s): Kamitani, S., et al.
  • Journal Title: The Journal of Biological Chemistry Volume: 285(33) Pages: 25467-25475
  • Peer Reviewed
• [Journal Article] Association of Bordetella dermonecrotic toxin with the extracellular matrix. (2010)
  • Author(s): Fukui-Miyazaki, A., Kamitani S, et al.
  • Journal Title: BMC Microbiology Volume: 10 Pages: 247-255
  • Peer Reviewed
• [Presentation] IVET-IP 法:ラット感染中の気管支敗血症菌の遺伝子発現プロファイル解析 (2013)
  • Author(s): 安倍裕順,神谷重樹,福井理,岡田圭祐,堀口安彦
  • Organizer: 第86回細菌学会総会
  • Place of Presentation: 千葉・幕張メッセ国際会議場
  • Year and Date: 2013-03-20
• [Presentation] 気管支敗血症菌の新たな病原因子 (2013)
  • Author(s): 西川明芳,安倍裕順,岡田圭祐,神谷重樹,福井理,堀口安彦
  • Organizer: 第86回細菌学会総会
  • Place of Presentation: 千葉・幕張メッセ国際会議場
  • Year and Date: 2013-03-20
• [Presentation] 気管支敗血症菌の新たな病原因子 (2013)
  • Author(s): 西川明芳、安倍裕順、岡田圭祐、神谷重樹、福井理、堀口安彦
  • Organizer: 第86回細菌学会総会
  • Place of Presentation: 千葉 幕張メッセ 国際会議場
• [Presentation] IVET-IP法：ラット感染中の気管支敗血症菌の遺伝子発現プロファイル解析 (2013)
  • Author(s): 安倍裕順、神谷重樹、福井理、岡田圭祐、堀口安彦
  • Organizer: 第86回細菌学会総会
  • Place of Presentation: 千葉 幕張メッセ 国際会議場
• [Presentation] IVET-IP system to screen Bordetella genes expressed in animal infection models (2012)
  • Author(s): 安倍裕順, 神谷重樹, 福井理, 戸嶋ひろ野, 堀口安彦
  • Organizer: 第85回日本細菌学会総会
  • Place of Presentation: 長崎・長崎ブリックホール/長崎新聞文化ホール
  • Year and Date: 2012-07-29
• [Presentation] 病原細菌の宿主特異性決定因子の解明 (2012)
  • Author(s): 安倍裕順,神谷重樹,堀口安彦,東秀明
  • Organizer: 第1回 人獣共通感染症研究拠点シンポジウム
  • Place of Presentation: 札幌・北海道大学
  • Year and Date: 2012-06-14
• [Presentation] Different activities of adenylate cyclase toxins of Bordetella pertussisand B. bronchiseptica (2012)
  • Author(s): 戸嶋ひろ野, 福井理, 安倍裕順, 神谷重樹 , 堀 口安 彦
  • Organizer: 第85回日本細菌学会総会
  • Place of Presentation: 長崎・長崎ブリックホール/長崎新聞文化ホー
  • Year and Date: 2012-03-29
• [Presentation] Different activities of adenylate cyclase toxins of Bordetella pertussis and Bordetella bronchiseptica (2012)
  • Author(s): 戸嶋ひろ野、福井理、安倍裕順、神谷重樹、堀口安彦
  • Organizer: 第85回日本細菌学会総会
  • Place of Presentation: 長崎ブリックホール長崎新聞文化ホール
  • Year and Date: 2012-03-27
• [Presentation] 病原細菌の宿主特異性決定因子の解明 (2012)
  • Author(s): 安倍裕順、神谷重樹、堀口安彦、東秀明
  • Organizer: 第1回 人獣共通感染症研究拠点シンポジウム
  • Place of Presentation: 北海道札幌 北海道大学
• [Presentation] 感染成立過程で発現する病原細菌遺伝子の網羅的解析法の開発 (2011)
  • Author(s): 安倍裕順, 神谷重樹, 福井理, 戸嶋ひろ野, 堀口安彦
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜・パシフィコ横浜
  • Year and Date: 2011-12-16
• [Presentation] ウェルシュ菌の産生する食中毒病原因子 CPE の構造および機能解析 (2011)
  • Author(s): 西村昂亮, 神谷重樹, 福井理, 戸嶋ひろ野, 安倍裕順, 鎌田洋一, 小西良子,山本茂貴, 柄谷肇, 堀口安彦, 北所健悟
  • Organizer: 第26回日本結晶学会年会
  • Year and Date: 2011-11-25
• [Presentation] THE PORE-FORMING MECHANISM OF CLOSTIRIDIUM PERFRINGENS ENTEROTOXIN TARGETING CLAUDINS, COMPONENTS OF THE TIGHT JUNCTION (2011)
  • Author(s): 堀口安彦, 北所健吾, 西村昂亮, 神谷重樹, 安倍裕順
  • Organizer: 第84回日本細菌学会総会
  • Place of Presentation: 札幌・札幌コンベンションセンター
  • Year and Date: 2011-09-10
• [Presentation] ウェルシュ菌エンテロトキシンの分子構造のβ型孔形成毒素の特徴 (2011)
  • Author(s): 北所健吾, 西村昂亮, 神谷重樹, 福井理, 戸嶋ひろ野, 安倍裕順, 鎌田洋一, 小西良子, 山本茂貴, 柄谷肇, 堀口安彦
  • Organizer: 第58回毒素シンポジウム
  • Place of Presentation: 東京・順天堂大学本郷キャンパス
  • Year and Date: 2011-07-07
• [Remarks]
  • URL: http://bactox1.biken.osaka-u.ac.jp/2011Web/Horiguchis_Lab_top.html