Identification of novel toxin genes in CA-MRSA
Project/Area Number |
22590403
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Bacteriology (including Mycology)
|
Research Institution | Juntendo University |
Principal Investigator |
|
Project Period (FY) |
2010 – 2012
|
Project Status |
Completed (Fiscal Year 2012)
|
Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
Keywords | MRSA / leukocidin / CA-MRSA / HA-MRSA / PVL / MW1941 / MW1942 / Staphylococcus aureus / MW1941/MW1942 / 白血球溶解 / Leukocidin |
Research Abstract |
Community-acquired MRSA (CA-MRSA) has a stronger virulence than Healthcare-assiciated MRSA. Production of Panton-Valentine Leukocidin (PVL) is deemed to be responsible for the strong virulence.However, it was not clear that PVL alone could explain the strong virulence of CA-MRSA. We noticed that CA-MRSA strain MW2 produced another set of leukocidins MW1941/1942. We expressed the toxins in E. coli, purified the toxin proteins and measured their lytic activity against human leukocytes. We observed a synergistic lytic activity of PVL and MW1941/1942 against human leukocytes.
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Report
(4 results)
Research Products
(3 results)