Identification of novel toxin genes in CA-MRSA

Research Project

Project/Area Number	22590403
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Bacteriology (including Mycology)
Research Institution	Juntendo University
Principal Investigator	HIRAMATSU Keiichi 順天堂大学, 医学部, 教授 (10173262)
Project Period (FY)	2010 – 2012
Project Status	Completed (Fiscal Year 2012)
Budget Amount *help	¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000) Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000) Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000) Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords	MRSA / leukocidin / CA-MRSA / HA-MRSA / PVL / MW1941 / MW1942 / Staphylococcus aureus / MW1941/MW1942 / 白血球溶解 / Leukocidin
Research Abstract	Community-acquired MRSA (CA-MRSA) has a stronger virulence than Healthcare-assiciated MRSA. Production of Panton-Valentine Leukocidin (PVL) is deemed to be responsible for the strong virulence.However, it was not clear that PVL alone could explain the strong virulence of CA-MRSA. We noticed that CA-MRSA strain MW2 produced another set of leukocidins MW1941/1942. We expressed the toxins in E. coli, purified the toxin proteins and measured their lytic activity against human leukocytes. We observed a synergistic lytic activity of PVL and MW1941/1942 against human leukocytes.

Report

(4 results)