Novel regulatory mechanisms against virus infection: activation of dignal transduction through protein SUMO modification

• Project/Area Number: 22590424
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Virology
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: KUBOTA Toru 国立感染症研究所, ウイルス第三部, 主任研究官 (10274929)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: ウイルス感染応答 / インターフェロン / SUMO化 / 翻訳後修飾 / SUMO / TBK1 / IKKε / TANK / ウイルス / 自然免疫 / タンパク質翻訳後修飾

Research Abstract

SUMO modification is one of the post-translational modification systems for regulating various cellular signal transductions. SUMO modification dependent molecular switch has been reported upon exhibiting innate immune response against virus infection. In this study, we focused on the contribution of SUMOylation upon virus infection induced type I IFN production pathway and found that PIASy negatively regulates both IFN transcription and IFN-stimulated gene expression through multiple mechanisms utilizing the function of different domains.

Research Products

• [Journal Article] Canine distemper virus with the intact C protein has the potential to replicate in human epithelial cells by using human nectin4 as a receptor. (2013)
  • Author(s): Noriyuki Otsuki
  • Journal Title: Virology Volume: 435(2) Pages: 485-492
  • Peer Reviewed
• [Journal Article] Dengue Virus Serotype 2 Blocks Extracellular Signal-Regulated Kinase and Nuclear Factor-κB Activation to Downregulate Cytokine Production (2012)
  • Author(s): Tsung-Hsien Chang
  • Journal Title: PLoS one Volume: 7(8)
  • Peer Reviewed
• [Journal Article] PIASy inhibits virus-induced and interferon-stimulated transcription through distinct mechanisms (2011)
  • Author(s): Kubota T, Matsuoka M, Xu S, Otsuki N, Takeda M, Kato A, Ozato K
  • Journal Title: Jounal of Biological Chemistry Volume: 286(10) Pages: 8165-75
  • Peer Reviewed
• [Journal Article] PIASy inhibits virus-induced and interferon-stimulated transcription through distinct mechanisms (2011)
  • Author(s): 久保田耐, 他
  • Journal Title: The Journal of Biological Chemistry Volume: 286 Pages: 8165-8175
  • Peer Reviewed
• [Journal Article] Piasy inhibits virus-induced and interferon-stimulated transcription through distinct mechanisms. (2011)
  • Author(s): Kubota T, Matsuoka M, Xu S, Otsuki N, Takeda M, Kato A, Ozato K.
  • Journal Title: The Journal of Biological Chemistry Volume: 286(10) Pages: 8165-8175
  • Peer Reviewed
• [Presentation] PIASY INHIBITS VIRUS INDUCED TYPE I INTERFERON PRODUCTION. (2010)
  • Author(s): Kubota T, Matsuoka M, Xu S, Otsuki N, Takeda M, Kato A, Ozato K.
  • Organizer: Cytokines Chicago 2010
  • Place of Presentation: アメリカ合衆国シカゴ
  • Year and Date: 2010-10-05
• [Presentation] Piasy inhibits virus induced type I Interferon production. (2010)
  • Author(s): Kubota T, Matsuoka M, Xu S, Takeda M, Shuai K, Kato A, Ozato K
  • Organizer: 8^<th> Joint Annual Meeting of the ISC/ISICR
  • Place of Presentation: Chicago, USA