Role of Rab proteins and related host factors in HIV-1 assembly

• Project/Area Number: 22590427
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Virology
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: MURAKAMI Tsutomu 国立感染症研究所, エイズ研究センター, 室長 (50336385)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: Rab 蛋白質 / Rab蛋白質 / HIV-1 / 細胞内輸送 / 粒子形成

Research Abstract

We hypothesized that some Rab proteins play roles in HIV-1 assembly and/or release. In the present study, we focused on Rab11, one of important regulators of recycling of cellular glycoproteins to the plasma membrane, and examined its potential roles in HIV-1 assembly. The results shown by the experiments using Rab11-kock down cells or cells that over-express Rab11 mutants suggest that Rab11 is involved in the formation of infectious virions by modulating trafficking of the HIV-1 Env glycoproteins.

Research Products

• [Presentation] R Functional analysis of Rab11a in HIV-1 assembly. (2012)
  • Author(s): Murakami T., H. Wu, M. Kawamata, J. Chiba, and T. Takemura
  • Organizer: The 2012 Meeting on Retroviruses
  • Place of Presentation: Cold Spring Harbor, USA
• [Presentation] Functional analysis of Rab11a in HIV-1 assembly. (2012)
  • Author(s): 村上 努
  • Organizer: 2012 Meeting on Retroviruses
  • Place of Presentation: Cold Spring Harbor, USA
• [Presentation] Functional analysis of Rab 11a in HIV-1 Replication (2011)
  • Author(s): Murakami T., H.Wu, M.Kawamata, K.Hayashi, J.Chiba, T.Takemura
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(横浜)
  • Year and Date: 2011-12-14
• [Presentation] Role of Rab11a in Virus Assembly of HIV-1 (2011)
  • Author(s): Murakami T., H.Wu, M.Kawamata, J.Chiba, T.Takemura
  • Organizer: XV International Congress of Virology
  • Place of Presentation: Sapporo convention Center, Sapporo
  • Year and Date: 2011-09-13
• [Presentation] Role of Rab11a in HIV-1 assembly (2011)
  • Author(s): Murakami T., H. Wu, M.Kawamata, J.Chiba, T.Takemura
  • Organizer: The 2011 Meeting on Retroviruses
  • Place of Presentation: Cold Spring Harbor, USA
  • Year and Date: 2011-05-25
• [Presentation] HIV-1 粒子形成過程におけるRab11a 蛋白質の機能解析 (2011)
  • Author(s): 呉 鴻規、竹村太地郎、川又美弥子、千葉丈、村上 努
  • Organizer: 第25回日本エイズ学会学術集会・総会
  • Place of Presentation: 東京
• [Presentation] Role of Rab11a in Virus Assembly of HIV-1. (2011)
  • Author(s): Murakami T., H. Wu, M. Kawamata, J. Chiba, and T. Takemera
  • Organizer: XV International Congress of Virology
  • Place of Presentation: Sapporo, Japan
• [Presentation] Role of Rab11a in HIV-1 assembly (2011)
  • Author(s): Murakami T., H. Wu, M. Kawamata, J. Chiba, and T. Takemura
  • Organizer: The 2011 Meeting on Retroviruses
  • Place of Presentation: Cold Spring Harbor, USA,
• [Presentation] Rab蛋白質のHIV-1複製における役割 (2010)
  • Author(s): 村上努、呉鴻規、川又美弥子、鶴岡幹大、千葉丈、竹村太地郎
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-10
• [Presentation] Roles of Rab proteins in HIV-1 assembly (2010)
  • Author(s): T.Murakami, H.Wu, M.Kawamata, M.Tsuruoka, J.Chiba, T.Takemura.
  • Organizer: The 10^<th> Awaji International Forum on Infection and Immunity
  • Place of Presentation: Awaji
  • Year and Date: 2010-09-09