Investigation of the defensive mechanisms of HIV-1 Vpu against the antiviral host factor BST-2

• Project/Area Number: 22590428
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Virology
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: TOKUNAGA Kenzo 国立感染症研究所, 感染病理部, 主任研究官 (50342895)
• Co-Investigator(Renkei-kenkyūsha): TATSUMI Masashi 国立感染症研究所, エイズ研究センター, 室長 (00133629) ; FUJITA Hideaki 九州大学, 薬学研究科, 助教 (80291524)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: HIV-1 / Vpu / BST-2 / 宿主因子

Research Abstract

HIV-1 Vpu enhances virion production by downregulating the antiviral host factor BST-2 from the cell surface. In this study, we attempted to identify host cofactors that play a supporting role in the anti-BST-2 activity of Vpu. We first prepared Vpu-binding proteins by performing immunoprecipitation assay, and identified multiple candidate proteins by proteome analyses. The candidate proteins were cloned into expression plasmids and then co-expressed with Vpu, followed by immunoprecipitation and subcellular localization assays. Knocking-down the gene expression of the candidate proteins that proved to interact with and colocalize with Vpu revealed no effect on Vpu’s anti-BST-2 activity, implying that those proteins might be involved in other functions of Vpu.

Research Products

• [Journal Article] DNA damage aids HIV-1 infection of macrophages by overcoming integrase inhibition. (2013)
  • Author(s): Koyama, T., Sun, B., Tokunaga, K., Tatsumi, M., and Ishizaka, Y
  • Journal Title: Retrovirology Volume: 10 Pages: 21-21
  • Peer Reviewed
• [Journal Article] Characteristics of IFITM, the newly identified IFN-inducible anti-HIV-1 family proteins. (2013)
  • Author(s): Chutiwitoonchai, N., Hiyoshi, M., Hiyoshi-Yoshidomi, Y., Hashimoto, M., Tokunaga, K., and Suzu, S
  • Journal Title: Microbes Infect Volume: 15 Pages: 280-90
  • Peer Reviewed
• [Journal Article] DNA damage enhances integration of HIV-1 into macrophages by overcoming integrase inhibition. (2013)
  • Author(s): Koyama, T.
  • Journal Title: Retrovirology Volume: 10 Issue: 1 Pages: 21-21
  • DOI: 10.1186/1742-4690-10-21
  • Peer Reviewed
• [Journal Article] Characteristics of IFITM, the newly identified IFN-inducible anti-HIV-1 family proteins. (2013)
  • Author(s): Chutiwitoonchai, N.
  • Journal Title: Microbes Infect. Volume: 15 Issue: 4 Pages: 280-90
  • DOI: 10.1016/j.micinf.2012.12.003
  • Peer Reviewed
• [Journal Article] Membrane-associated RING-CH (MARCH) 8 mediates the ubiquitination and lysosomal degradation of the transferrin receptor. (2013)
  • Author(s): Fujita, H.
  • Journal Title: J Cell Sci. Volume: in press
  • DOI: 10.1242/jcs.119909
  • Peer Reviewed
• [Journal Article] HIV-1 Vpu and BST-2/tetherin: Enemies at the Gates. (2012)
  • Author(s): Tokunaga, K
  • Journal Title: Current HIV Res Volume: 10 Pages: 275-276
• [Journal Article] Sites of action of HIV-1 Vpu in BST-2/tetherin downregulation. (2012)
  • Author(s): Arias, J.F., Iwabu, Y., and Tokunaga, K
  • Journal Title: Current HIV Res Volume: 10 Pages: 283-291
  • Peer Reviewed
• [Journal Article] Intracellular Logistics of BST-2/Tetherin. (2012)
  • Author(s): Fujita, H., Fujimoto, K., Tokunaga, K., and Tanaka, Y
  • Journal Title: Current HIV Res Volume: 10 Pages: 321-326
  • Peer Reviewed
• [Journal Article] proteins: No great escape from the magnificent seven. (2012)
  • Author(s): Arias, J.F., Koyama, T., Kinomoto, M., and Tokunaga, K
  • Journal Title: Front Microbiol Volume: 3 Pages: 275-275
  • Peer Reviewed
• [Journal Article] Host Restriction Factors in Retroviral Infection: Promises in Virus-Host Interaction. (2012)
  • Author(s): Zheng, Y.-H., Jeang, K.-T., and Tokunaga, K
  • Journal Title: Retrovirology Volume: 9 Pages: 112-112
  • Peer Reviewed
• [Journal Article] Editorial: HIV-1 Vpu and BST-2/tetherin: Enemies at the Gates. (2012)
  • Author(s): Tokunaga, K.
  • Journal Title: Current HIV Res. Volume: 10 Pages: 275-6
• [Journal Article] Sites of action of HIV-1 Vpu in BST-2/tetherin downregulation. (2012)
  • Author(s): Arias, J.F.
  • Journal Title: Current HIV Res. Volume: 10 Pages: 283-91
  • Peer Reviewed
• [Journal Article] Intracellular Logistics of BST-2/Tetherin. (2012)
  • Author(s): Fujita, H.
  • Journal Title: Current HIV Res. Volume: 10 Pages: 321-6
  • Peer Reviewed
• [Journal Article] Retroelements versus APOBEC3 family members: No great escape from the magnificent seven. (2012)
  • Author(s): Arias, J.F.
  • Journal Title: Front Microbiol. Volume: 3 Pages: 56-62
  • DOI: 10.3389/fmicb.2012.00275
  • Peer Reviewed
• [Journal Article] Host restriction factors in retroviral infection: promises in virus-host interaction. (2012)
  • Author(s): Zheng, Y.H.
  • Journal Title: Retrovirology Volume: 9 Issue: 1 Pages: 112-112
  • DOI: 10.1186/1742-4690-9-112
  • Peer Reviewed
• [Journal Article] Intracellular Logistics of BST-2/Tetherin (2012)
  • Author(s): Fujita, H., Fujimoto, K., Tokunaga, K., Tanaka, Y.
  • Journal Title: Current HIV Res. Volume: (in press)
  • Peer Reviewed
• [Journal Article] Sites of action of HIV-1 Vpu in BST-2/tetherm downregulation (2012)
  • Author(s): Arias, J.A., Iwabu, Y., Tokunaga, K(corresponding author)
  • Journal Title: Current HIV Res. Volume: (in press)
  • Peer Reviewed
• [Journal Article] Editorial-HIV-1 Vpu and BST-2/tetherin : Enemies at the Gates (2012)
  • Author(s): Tokunaga, K.(Guest Editor)
  • Journal Title: Current HIV Res. Volume: (編集後記)(in press)
• [Journal Article] protein complex 2 alpha 1 subunit-mediated regulation of human immunodeficiency virus type 1 replication. (2011)
  • Author(s): Sapsutthipas, S., Kitagawa, Y., Tokunaga, K., Ikuta, K., and Kameoka, M
  • Journal Title: Southeast Asian J. Trop. Med. Public Health. Volume: 42 Pages: 311-319
  • Peer Reviewed
• [Journal Article] HIV-1感染阻害因子(HIV-1 Restriction Factors) (2011)
  • Author(s): 徳永研三、足立昭夫、高折晃史、中山英美、岩部幸枝、岩谷靖雄
  • Journal Title: 日本エイズ学会誌 The Journal of AIDS Research. Volume: 13 Pages: 56-62
• [Journal Article] Identification of SNF2h, a Chromatin-Remodeling Factor, as a Novel Binding Protein of Vpr of Human Immunodeficiency Virus Type 1. (2011)
  • Author(s): Taneichi, D., Iijima, K., Doi, A., Koyama, T., Minemoto, Y., Tokunaga, K., Shimura, M., and Ishizaka, Y
  • Journal Title: J. Neuroimmune Pharm Volume: 6 Pages: 177-87
  • Peer Reviewed
• [Journal Article] Intrinsic restriction activity by apolipoprotein B mRNA editing enzyme APOBEC1 against the mobility of autonomous retrotransposons. (2011)
  • Author(s): Ikeda, T., Abd El Galil, K., Tokunaga, K., Maeda, K., Sata, T., Sakaguchi, N., Harada, S., Heidmann, T., and Koito, A
  • Journal Title: Nucleic Acids Res Volume: 39 Pages: 5538-5554
  • Peer Reviewed
• [Journal Article] Epigenetic displacement of HP1 from heterochromatin by HIV-1 Vpr causes premature sister chromatid separation. (2011)
  • Author(s): Shimura, M., Toyoda, Y., Iijima, K., Kinomoto, M, Tokunaga, K., Yoda, K., Yanagida, M., Sata, T., and Ishizaka, Y
  • Journal Title: J. Cell Biol Volume: 194 Pages: 721-35
  • Peer Reviewed
• [Journal Article] Structural basis for antiviral activity of BST-2/tetherin and its viral antagonisms. (2011)
  • Author(s): Arias, J.F., Iwabu, Y., and Tokunaga, K
  • Journal Title: Front Microbiol Volume: 2 Pages: 250-250
  • Peer Reviewed
• [Journal Article] Identification of SNF2h, a Chromatin-Remodeling Factor, as a Novel Binding Protein of Vpr of Human Immunodeficiency Virus Type 1 (2011)
  • Author(s): Taneichi, D., Iijima, K., Doi, A., Koyama, T., Minemoto, Y., Tokunaga, K., Shimura, M., Ishizaka, Y.
  • Journal Title: J.Neuroimmune Pharm. Volume: 6 Issue: 2 Pages: 177-87
  • DOI: 10.1007/s11481-011-9276-5
  • Peer Reviewed
• [Journal Article] Viral factors involved in adapter-related protein complex 2 alpha 1 subunit-mediated regulation of human immunodeficiency virus type 1 replication (2011)
  • Author(s): Sapsutthipas, S., Kitagawa, Y., Tokunaga, K., Ikuta, K., Kameoka, M.
  • Journal Title: Southeast Asian J.Trop.Med.Public Health. Volume: 42 Pages: 311-9
  • Peer Reviewed
• [Journal Article] Intrinsic restriction activity by apolipoprotein B mRNA editing enzyme APOBEC1 against the mobility of autonomous retrotransposons (2011)
  • Author(s): Ikeda, T., Abd El Galil, K., Tokunaga, K., Maeda, K., Sata, T., Sakaguchi, N., Harada, S., Heidmann, T., Koito, A.
  • Journal Title: Nucleic Acids Res Volume: 39 Issue: 13 Pages: 5538-54
  • DOI: 10.1093/nar/gkr124
  • Peer Reviewed
• [Journal Article] Epigenetic displacement of HP1 from heterochromatin by HIV-1 Vpr causes premature sister chromatid separation (2011)
  • Author(s): Shimura, M., Toyoda, Y., Iijima, K., Kinomoto, M, Tokunaga, K., Yoda, K., Yanagida, M., Sata, T., Ishizaka, Y.
  • Journal Title: J.Cell Biol. Volume: 194 Issue: 5 Pages: 721-35
  • DOI: 10.1083/jcb.201010118
  • Peer Reviewed
• [Journal Article] Structural basis for antiviral activity of BST-2/tetherin and its viral antagonisms (2011)
  • Author(s): Arias, J.A., Iwabu, Y., Tokunaga, K(corresponding author)
  • Journal Title: Front.Microbiol. Volume: 2 Pages: 250-250
  • DOI: 10.3389/fmicb.2011.00250
  • Peer Reviewed
• [Journal Article] HIV-1感染阻害因子(HIV-1 Restriction Factors) (2011)
  • Author(s): 徳永研三、足立昭夫、高折晃史、中山英美、岩部幸枝、岩谷靖雄
  • Journal Title: 日本エイズ学会誌The Journal of AIDS Research Volume: 13 Pages: 56-62
• [Journal Article] Intrinsic restriction activity by apolipoprotein B mRNA editing enzyme APOBEC1 against the mobility of autonomous retrotransposons. (2011)
  • Author(s): Ikeda, T., Abd El Galil K., Tokunaga, K., Maeda, K., Sata, T., Sakaguchi, N., Harada, S., Heidmann, T., Koito, A.
  • Journal Title: Nucleic Acids Res. Volume: in press
  • Peer Reviewed
• [Journal Article] Identification of SNF2h, a Chromatin-Remodeling Factor, as a Novel Binding Protein of Vpr of Human Immunodeficiency Virus Type 1. (2011)
  • Author(s): Taneichi, D., Iijima, K., Doi, A., Koyama, T., Minemoto, Y., Tokunaga, K., Shimura, M., Ishizaka, Y.
  • Journal Title: J.Neuroimmune Pharm. Volume: in press
  • Peer Reviewed
• [Journal Article] Two N-linked glycosylation sites in V2 and C2 regions of human immunodeficiency virus type 1 CRF01_AE envelope glycoprotein gp120 regulate viral neutralization susceptibility to the human monoclonal antibody specific for CD4 binding domain. (2010)
  • Author(s): Utachee, P., Nakamura, S., Isarangkura-na-ayuthaya, P., Tokunaga, K., Sawanpanyalert, P., Ikuta, K., Auwanit, W., and Kameoka, M
  • Journal Title: J. Virol Volume: 84 Pages: 4311-20
  • Peer Reviewed
• [Journal Article] Combined analysis of cell growth and apoptosis-regulating proteins in HPVs associated anogenital tumors. (2010)
  • Author(s): Mitsuishi, T., Iwabu, Y., Tokunaga, K., Sata, T., Kaneko, T., Ohara, K., Ohsawa, I., Oda, F., Kato, T., Yamada, Y., Kawana, S., Ozaki, K., Nakatake, M., and Yamada, O
  • Journal Title: BMC cancer Volume: 10 Pages: 118-118
  • Peer Reviewed
• [Journal Article] BST-2/tetherin by the HIV-1 accessory protein Vpu (2010)
  • Author(s): Iwabu, Y., Fujita, H., Tanaka, Y., Sata, T., and Tokunaga, K
  • Journal Title: Commun. Integr. Biol Volume: 3 Pages: 366-369
  • Peer Reviewed
• [Journal Article] The role of lysine residue at amino acid position 165 of human immunodeficiency virus type 1 CRF01_AE Gag in reducing viral drug susceptibility to protease inhibitors. (2010)
  • Author(s): Kameoka, M, Isarangkura-na-ayuthaya, P., Kameoka, Y., Sapsutthipas, S., Soonthornsata, B., Nakamura, S., Tokunaga, K., Sawanpanyalert, P., Ikuta, K., and Auwanit, W.
  • Journal Title: Virology Volume: 405 Pages: 129-38
  • Peer Reviewed
• [Journal Article] Differential anti-APOBEC3G activity of HIV-1 Vif proteins derived from different subtypes. (2010)
  • Author(s): Iwabu, Y., Kinomoto, M., Tatsumi, M., Fujita, H., Shimura, M., Tanaka, Y., Ishizaka, Y., Nolan, D., Mallal, S., Sata, T., and Tokunaga, K (corresponding author)
  • Journal Title: J. Biol. Chem Volume: 285 Pages: 35350-35358
  • Peer Reviewed
• [Journal Article] Two N-linked glycosylation sites in V2 and C2 regions of human immunodeficiency virus type 1 CRFO1_AE envelope glycoprotein gp120 regulate viral neutralization susceptibility to the human monoclonal antibody specific for CD4 binding domain. (2010)
  • Author(s): Utachee, P., Nakamura, S., Isarangkura-na-ayuthaya, P., Tokunaga, K., Sawanpanyalert, P., Ikuta, K., Auwanit W., Kameoka, M.
  • Journal Title: J.Virol. Volume: 84 Pages: 4311-4320
  • Peer Reviewed
• [Journal Article] Combined analysis of cell growth and apoptosis-regulating proteins in HPVsassociated anogenital tumors. (2010)
  • Author(s): Mitsuishi, T., Iwabu, Y., Tokunaga, K., Sata, T., Kaneko, T., Ohara, K., Ohsawa, I., Oda, F., Kato, T., Yamada, Y., Kawana, S., Ozaki, K., Nakatake, M., Yamada, O.
  • Journal Title: BMC cancer Volume: 10 Pages: 118-118
  • Peer Reviewed
• [Journal Article] Direct internalization of cell-surface BST-2/tetherin by the HIV-1 accessory protein Vpu. (2010)
  • Author(s): Iwabu, Y., Fujita, H., Tanaka, Y., Sata, T., Tokunaga, K(corresponding author).
  • Journal Title: Commurr.Integr.Biol. Volume: 3 Pages: 366-369
  • Peer Reviewed
• [Journal Article] The role of lysine residue at amino acid position 165 of human immunodeficiency virus type 1 CRF01_AE Gag in reducing viral drug susceptibility to protease inhibitors. (2010)
  • Author(s): Kameoka, M., Isarangkura-na-ayuthaya, P., Kameoka, Y., Sapsutthipas, S., Soonthornsata, B., Nakamura, S., Tokunaga, K., Sawanpanyalert, P., Ikuta, K., Auwanit, W.
  • Journal Title: Virology Volume: 405 Pages: 129-138
  • Peer Reviewed
• [Journal Article] Differential anti-APOBEC3G activity of HIV-1 Vif proteins derived fromdifferent subtypes. (2010)
  • Author(s): Iwabu, Y., Kinomoto, M., Tatsumi, M., Fujita, H., Shimura, M., Tanaka, Y., Ishizaka, Y., Nolan, D., Mallal, S., Sata, T., Tokunaga, K(corresponding author).
  • Journal Title: J.Biol.Chem. Volume: 285 Pages: 35350-35358
  • Peer Reviewed
• [Journal Article] Membrane-associated RING-CH (MARCH) 8 mediates the ubiquitination and lysosomal degradation of the transferrin receptor.
  • Author(s): Fujita, H., Iwabu, Y., Tokunaga, K., and Tanaka, Y
  • Journal Title: J. Cell Sci. Volume: (in press)
  • Peer Reviewed
• [Presentation] Anti-APOBEC3G activity of HIV-1 Vif protein from elite controllers is attenuated compared to those from untreated chronic progressors or those from individuals with acute infection. (2012)
  • Author(s): T. Kikuchi, Y. Iwabu, A. Kawana- Tachikawa, M. Koga, N. Hosoya, S. Nomura1, Z.L. Brumme, H. Jessen, A. Kelleher, M. Markowitz, F. Pereyra, A. Trocha, B.D. Walker, A. Iwamoto, K. Tokunaga, and T. Miura
  • Organizer: XIX International AIDS Conference
  • Place of Presentation: Washington D.C., USA
• [Presentation] HIV-1感染者における抗ウイルス宿主因子の発現レベルと病態進行との相関性の有無 (2012)
  • Author(s): 張 延昭、岩部幸枝、立川(川名) 愛、中村仁美、David Nolan、Simon Mallal、長谷川秀樹、山岡昇司、岩本愛吉、徳永研三
  • Organizer: 第59回日本ウイルス学会総会
  • Place of Presentation: 大阪
• [Presentation] HIV-2 Vpxに不活化される抗ウイルス宿主因子の探索 (2012)
  • Author(s): 小山貴芳、Juan F. Arias、岩部幸枝、徳永研三
  • Organizer: 第59回日本ウイルス学会総会
  • Place of Presentation: 大阪
• [Presentation] APOBEC3GによるAlu転移抑制の分子生物学的および構造学的解析 (2012)
  • Author(s): Juan F. Arias、小山貴芳、岩部幸枝、横山勝、佐藤裕徳、長谷川秀樹、徳永研三
  • Organizer: 第59回日本ウイルス学会総会
  • Place of Presentation: 大阪
• [Presentation] HIV-1 elite controllerにおけるHIV-1 Vifの抗APOBEC3G活性の低下 (2012)
  • Author(s): 菊地正、岩部幸枝、立川(川名)愛、古賀道子、野村滋、細谷紀彰、Zabrina L. Brumme、Heiko Jessen、Anthony D. Kelleher、Martin Markowitz、Florencia Pereyra、Alicja Trocha、Bruce D. Walker、岩本愛吉、徳永研三、三浦聡之
  • Organizer: 第59回日本ウイルス学会総会
  • Place of Presentation: 大阪
• [Presentation] APOBEC3Gの抗Aluレトロ転移活性に寄与するアミノ酸の同定 (2012)
  • Author(s): 小山貴芳、Juan F Arias、横山勝、佐藤裕徳、徳永研三
  • Organizer: 第35回日本分子生物学会
  • Place of Presentation: 福岡
• [Presentation] HIV-1感染者における抗ウイルス宿主因子の発現レベルと病態進行との相関性の有無． (2012)
  • Author(s): 張延昭
  • Organizer: 第60回日本ウイルス学会学術集会
  • Place of Presentation: 大阪
• [Presentation] HIV-2 Vpxに不活化される抗ウイルス宿主因子の探索． (2012)
  • Author(s): 小山貴芳
  • Organizer: 第60回日本ウイルス学会学術集会
  • Place of Presentation: 大阪
• [Presentation] APOBEC3GによるAlu転移抑制の分子生物学的および構造学的解析． (2012)
  • Author(s): 徳永研三
  • Organizer: 第60回日本ウイルス学会学術集会
  • Place of Presentation: 大阪
• [Presentation] HIV-1 elite controllerにおけるHIV-1 Vifの抗APOBEC3G活性の低下． (2012)
  • Author(s): 菊地正
  • Organizer: 第60回日本ウイルス学会学術集会
  • Place of Presentation: 大阪
• [Presentation] APOBEC3Gの抗Aluレトロ転移活性に寄与するアミノ酸の同定． (2012)
  • Author(s): 小山貴芳
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
• [Presentation] Infectivity of HIV-1 Subtypes Isolated from Ghanaian Patients. (2011)
  • Author(s): Barnor, J., Tatsumi, M., Sata, T., Ampofo, W., Osei-Kwasi, M., Tokunaga, K
  • Organizer: Patients.WSU Joint International Conference. East London
  • Place of Presentation: South Africa
• [Presentation] HIV-1 DNA integration into host chromosomal double-strand break sites is not attenuated by raltegravir, an integrase inhibitor. (2011)
  • Author(s): Takayoshi Koyama, Kenzo Tokunaga, Tetsutaro Sata, Yukihito Ishizaka
  • Organizer: IUMS 2011
  • Place of Presentation: Sapporo, Japan,
• [Presentation] 膜結合型ユビキチンリガーゼMARCH8によるトランスフェリン受容体のユビキチン化およびダウンレギュレーションの分子機構 (2011)
  • Author(s): 藤田英明、岩部幸枝、佐多徹太郎、徳永研三、田中嘉孝
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都
• [Presentation] Homodimerization of APOBEC3G is required for inhibition of Alu retrotransposition. (2011)
  • Author(s): Yukie Iwabu, Juan F. Arias, Masaru Yokoyama, Hironori Sato, Tetsutaro Sata and Kenzo Tokunaga
  • Organizer: Frontiers of Retrovirology Conference 2011,
  • Place of Presentation: Amsterdam, The Netherlands
• [Presentation] HIV-1 integraion into host DNA double-strand break sites is the majority event in integrase inhibitor-treated cells. (2011)
  • Author(s): Takayoshi Koyama, Kenzo Tokunaga, Yukihito Ishizaka
  • Organizer: 第34回日本分子生物学会
  • Place of Presentation: 横浜
• [Presentation] Infectivity of HIV-1 Subtypes Isolated from Ghanaian Patients (2011)
  • Author(s): Nii-Trebi, N., Kinomoto, M., Brandful, J., Barnor, J., Tatsumi, M., Sata, T., Ampofo, W., Osei-Kwasi, M., Tokunaga, K.
  • Organizer: WSU Joint International Conference
  • Place of Presentation: East London, South Africa
• [Presentation] HIV-1 DNA integration into host chromosomal double-strand break sites is not attenuated by raltegravir, an integrase inhibitor (2011)
  • Author(s): Takayoshi Koyama, Kenzo Tokunaga, Tetsutaro Sata, Yukihito Ishizaka
  • Organizer: IUMS 2011
  • Place of Presentation: Sapporo, Japan
• [Presentation] Homodimerization of APOBEC3G is required for inhibition of Aluretrotransposition (2011)
  • Author(s): Yukie Iwabu, Juan F.Arias, Masaru Yokoyama, Hironori Sato, Tetsutaro Sata, Kenzo Tokunaga
  • Organizer: Frontiers of Retrovirology Conference 2011
  • Place of Presentation: Amsterdam, The Netherlands
• [Presentation] HIV-1 integraion into host DNA double-strand break sites is the majority event in integrase inhibitor-treated cells (2011)
  • Author(s): Takayoshi Koyama, Kenzo Tokunaga, Yukihito Ishizaka
  • Organizer: 第34回日本分子生物学会
  • Place of Presentation: 横浜
• [Presentation] Direct internalization of cell-surface BST-2/tetherin by HIV-1 Vpu. (2010)
  • Author(s): Yukie Iwabu, Hideaki Fujita, Yoshitaka Tanaka, Tetsutaro Sata, and Kenzo Tokunaga
  • Organizer: Centennial Retroviruses Meeting (CRM2010)
  • Place of Presentation: Prague, Czech Republic
• [Presentation] Integration into DNA double-strand break sites of HIV-1 is not attenuated by raltegravir, an integrase inhibitor. (2010)
  • Author(s): akayoshi Koyama, Kenzo Tokunaga, Tetsutaro Sata, Yukihito Ishizaka
  • Organizer: 11th Kumamoto AIDS Seminar Global COE Joint International Symposium
  • Place of Presentation: Kumamoto
• [Presentation] Battles between Host and Viruses: Molecular Mechanisms of the Inactivation of Antiviral Host Factors by HIV-1 Accessory Proteins (2010)
  • Author(s): Kenzo Tokunaga
  • Organizer: National Institute of Health, Thailand.
  • Place of Presentation: Bangkok, Thailand
• [Presentation] Targeting of recycling endosomes to the cleavage furrow requires ubiquitylation of membrane proteins. (2010)
  • Author(s): Hideaki Fujita, Yukie Iwabu, Kenzo Tokunaga, Tetsutaro Sata, Keiko Fujimoto, Ryo Tachiyama, Daisuke Ishikawa, Masaki Matsumoto, Keiichi I Nakayama, and Yoshitaka Tanaka
  • Organizer: Biochemistry and Cell Biology of ESCRTs in Health and Disease (ASBMB Special Meeting)
  • Place of Presentation: Utah, USA.
• [Presentation] HIV-1 VpuによるBST-2/tetherinの機能阻害に関与するcofactorの検索 (2010)
  • Author(s): 岩部幸枝、藤田英明、田中嘉孝、佐多徹太郎、徳永研三
  • Organizer: 第58回日本ウイルス学会総会
  • Place of Presentation: 徳島
• [Presentation] HIV-1 Vpuによる細胞表面BST-2/tetherinの直接的なinternalization (2010)
  • Author(s): 徳永研三、岩部幸枝、藤田英明、田中嘉孝、佐多徹太郎
  • Organizer: 第58回日本ウイルス学会総会
  • Place of Presentation: 徳島
• [Presentation] CRF01_AE Gag 165番目のリジン残基がプロテアーゼ阻害剤に対するウイルスの薬剤感受性を低下させる分子機構 (2010)
  • Author(s): 亀岡正典、Panasda Isarangkura-na- ayuthaya、亀岡陽子、Sompong Sapsutthipas、Bongkot Soonthornsata、中村昇太、徳永研三、Pathom Sawanpanyalert、生田和良、Wattana Auwanit
  • Organizer: 第58回日本ウイルス学会総会
  • Place of Presentation: 徳島
• [Presentation] Two N-linked glycosylation sites in Env gp120 regulate the susceptibility of CRF01_AE viruses to the CD4 binding domain antibody, IgG1 b12. (2010)
  • Author(s): Piraporn Utachee、中村昇太、Panasda Isarangkura-na-ayuthaya、徳永研三、Pathom Sawanpanyalert、生田和良、Wattana Auwanit、亀岡正典
  • Organizer: 第58回日本ウイルス学会総会
  • Place of Presentation: 徳島
• [Presentation] 哺乳類APOBEC1による内在性レトロエレメント制御機構の解析 (2010)
  • Author(s): 池田輝政、Abd El Galil Khaled Hussein、徳永研三、前田和彦、佐多徹太郎、阪口薫雄、Thierry Heidman、小糸厚
  • Organizer: 第58回日本ウイルス学会総会
  • Place of Presentation: 徳島
• [Presentation] BST-2/tetherin (2010)
  • Author(s): 岩部幸枝,佐多徹太郎,徳永研三
  • Organizer: 第24回日本エイズ学会シンポジウム[Restriction Factor]
  • Place of Presentation: 東京
• [Presentation] APOBEC3Gの抗Aluレトロ転移活性に関わる責任領域の同定 (2010)
  • Author(s): 岩部幸枝,佐多徹太郎,徳永研三
  • Organizer: 第33回日本分子生物学会
  • Place of Presentation: 神戸
• [Presentation] BST-2/tetherinのdownregulationにおけるHIV-1 Vpuのcofactor要求性 (2010)
  • Author(s): 岩部幸枝、藤田英明、田中嘉孝、佐多徹太郎、徳永研三
  • Organizer: 第33回日本分子生物学会
  • Place of Presentation: 神戸
• [Presentation] The endosomal vesicles carrying ubiquitylated cargos selectively localizes to the cleavage furrow during cytokinesis (2010)
  • Author(s): Hideaki Fujita, Yukie Iwabu, Kenzo Tokunaga, Tetsutaro Sata, Keiko Fujimoto, Ryo Tachiyama, Daisuke Ishikawa, Masaki Matsumoto, Keiichi I Nakayama, and Yoshitaka Tanaka
  • Organizer: 第33回日本分子生物学会
  • Place of Presentation: 神戸
• [Presentation] Site-Specific Integration of a Lentiviral Vector in the Human Genome via DNA Double-Strand Breaks. (2010)
  • Author(s): Takayoshi Koyama, Kenzo Tokunaga, Tetsutaro Sata, Yukihito Ishizaka
  • Organizer: 第33回日本分子生物学会
  • Place of Presentation: 神戸
• [Presentation] Direct internalization of cell-surface BST-2/tetherin by HIV-1 Vpu. (2010)
  • Author(s): Yukie Iwabu, Hideaki Fujita, Yoshitaka Tanaka, Tetsutaro Sata, Kenzo Tokunaga (presenter)
  • Organizer: Centennial Retroviruses Meeting (CRM2010)
  • Place of Presentation: Prague, Czech Republic.
• [Presentation] Battles between Host and Viruses : Molecular Mechanisms of the Inactivation of Antiviral Host Factors by HIV- 1 Accessory Proteins. (2010)
  • Author(s): Kenzo Tokunaga
  • Organizer: Thai NIH Seminar
  • Place of Presentation: Thailand.Bangkok
• [Presentation] Targeting of recycling endosomes to the cleavage furrow requires ubiquitylation of membrane proteins. (2010)
  • Author(s): Hideaki Fujita, Yukie Iwabu, Kenzo Tokunaga, Tetsutaro Sata, Keiko Fujimoto, Ryo Tachiyama, Daisuke Ishikawa, Masaki Matsumoto, Keiichi I Nakayama, Yoshitaka Tanaka.
  • Organizer: Biochemistry and Cell Biology of ESCRTs in Health and Disease (ASBMB Special Meeting)
  • Place of Presentation: Utah, USA
• [Presentation] HIV-1 Vpuによる細胞表面BST-2/tetherinの直接的なinternalization. (2010)
  • Author(s): 徳永研三、岩部幸枝、藤田英明、田中嘉孝、佐多徹太郎
  • Organizer: 第58回日本ウイルス学会総会
  • Place of Presentation: 徳島
• [Presentation] CRFO1_AE Gag 165番目のリジン残基がプロテアーゼ阻害剤に対するウイルスの薬剤感受性を低下させる分子機構 (2010)
  • Author(s): 亀岡正典、Panasda Isarangkura-na-ayuthaya、亀岡陽子、Sompong Sapsutthipas、Bongkot Soonthornsata、中村昇太、徳永研三、Pathom Sawanpanyalert、生田和良、Wattana Auwanit
  • Organizer: 第58回日本ウイルス学会総会
  • Place of Presentation: 徳島
• [Presentation] Two N-linked glycosylation sites in Env gp 120 regulate the susceptibility of CRF01_AE viruses to the CD4 binding domain antibody, IgGl b12. (2010)
  • Author(s): Piraporn Utachee、中村昇太、Panasda Isarangkura-na-ayuthaya、徳永研三、Pathom Sawanpanyalert、生田和良、Wattana Auwanit、亀岡正典
  • Organizer: 第58回日本ウイルス学会総会
  • Place of Presentation: 徳島
• [Presentation] BST-2/tetherin. (2010)
  • Author(s): 岩部幸枝, 佐多徹太郎, 徳永研三
  • Organizer: 第24回日本エイズ学会シンポジウム[Restriction Factor]
  • Place of Presentation: 東京
• [Presentation] BST-2/tetherinのdownregulationにおけるHIV-1Vpuのcofactor要求性 (2010)
  • Author(s): 岩部幸枝、藤田英明、田中嘉孝、佐多徹太郎、徳永研三
  • Organizer: 第33回日本分子生物学会
  • Place of Presentation: 神戸
• [Presentation] The endosomal vesicles carrying ubiquitylated cargos selectively localizes to the cleavage furrow during cytokinesis. (2010)
  • Author(s): Hideaki Fujita, Yukie Iwabu, Kenzo Tokunaea, Tetsutaro Sata, Keiko Fujimoto, Ryo Tachiyama, Daisuke Ishikawa, Masaki Matsumoto, Keiichi I Nakayama, Yoshitaka Tanaka
  • Organizer: 第33回日本分子生物学会
  • Place of Presentation: 神戸