PI3-K plays a critical role in the class switch recombination of immunoglobulin

• Project/Area Number: 22590437
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Immunology
• Research Institution: Keio University
• Principal Investigator: FUJII Hideki 慶應義塾大学, 医学部, 助教 (50356250)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: イムノグロブリン / クラススイッチ / PI3K / IL4 / LPS / B細胞 / Akt / IL-4 / サイトカイン

Research Abstract

In this study, we investigated the role of PI3K in B cells. p85αdeficiency inhibited IgG3 antibody production stimulated with lipopolysaccharide (LPS) by using B cells derived from p85α-/-mice. We next evaluate the expression of germ line transcription (G

Research Products

• [Journal Article] Influenza A whole virion vaccine induces a rapid reduction of peripheral blood leukocytes via interferon-α-dependent apoptosis. (2013)
  • Author(s): Ato M, Takahashi Y, Fujii H, Hashimoto S, Kaji T, Itamura S, Horiuchi Y, Arakawa Y, Tashiro M, Takemori T.
  • Journal Title: Vaccine. Volume: 31(17) Issue: 17 Pages: 2184-90
  • DOI: 10.1016/j.vaccine.2013.02.016
  • Peer Reviewed
• [Journal Article] mTORC1 in Intestinal CD11c+CD11b+Dendritic Cells Regulates Intestinal Homeostasis by Promoting IL-10 Production (2012)
  • Author(s): Ohtani M, Hoshii T, Fujii H, Koyasu S, Hirao A, Matsuda S.
  • Journal Title: J Immunol Volume: 188 Issue: 10 Pages: 4736-40
  • DOI: 10.4049/jimmunol.1200069
  • Peer Reviewed
• [Journal Article] Langerhans cell antigen capture through tight junctions confers preemptive immunity in experimental staphylococcal scalded skin syndrome (2011)
  • Author(s): Ouchi T
  • Journal Title: J Exp Med Volume: 208 Issue: 13 Pages: 2607-2613
  • DOI: 10.1084/jem.20111718
  • Peer Reviewed
• [Journal Article] Desmoglein 3-specific CD4+ T cells induce pemphigus vulgaris and interface dermatitis in mice (2011)
  • Author(s): Takahashi H, Kouno M, Nagao K, Wada N, Hata T, Nishimoto S, 他
  • Journal Title: J Clin Invest Volume: 121 Issue: 9 Pages: 3677-3688
  • DOI: 10.1172/jci57379
  • Peer Reviewed
• [Journal Article] HIV-1 Nef impairs multiple T-cell functions in antigen-specific immune response in mice (2011)
  • Author(s): Fujii, H., Ato, M., Takahashi, Y., Otake, K., Hashimoto, S., Kaji, T., Tsunetsugu-Yokota, Y., Fujita, M., Adachi, A., Nakayama, T., Taniguchi, M. Koyasu, S., Takemori, T.
  • Journal Title: Int.Immunol. Volume: 23 Issue: 7 Pages: 433-441
  • DOI: 10.1093/intimm/dxr031
  • Peer Reviewed
• [Journal Article] Diet-induced adipose tissue inflammation and liver steatosis are prevented by DPP-4 inhibition in diabetic mice (2011)
  • Author(s): Shirakawa J, Fujii H, Ohnuma K, Sato K, Ito Y, Kaji M, Sakamoto E, Koganei M, Sasaki H, Nagashima Y, Amo K, Aoki K, Morimoto C, Takeda E, Terauchi Y
  • Journal Title: Diabetes Volume: 60(4) Issue: 4 Pages: 246-1257
  • DOI: 10.2337/db10-1338
  • Peer Reviewed
• [Journal Article] Innate production of T(H)2 cytokines by adipose tissue-associated c-Kit(+)Sca-1(+) lymphoid cells. (2010)
  • Author(s): Moro K, Yamada T, Tanabe M, Takeuchi T, Ikawa T, Kawamoto H, Furusawa J, Ohtani M, Fujii H, Koyasu S.
  • Journal Title: Nature. Volume: 463(7280) Issue: 7280 Pages: 540-4
  • DOI: 10.1038/nature08636
  • Peer Reviewed
• [Journal Article] Thymoproteasome shapes immunocompetent repertoire of CD8+ T cells. (2010)
  • Author(s): Nitta T, Murata S, Sasaki K, Fujii H, Mat Ripen A, Ishimaru N, Koyasu S, Tanaka K, Takahama Y
  • Journal Title: Immunity Volume: 32 Issue: 1 Pages: 29-40
  • DOI: 10.1016/j.immuni.2009.10.009
  • NAID: 40017340869
  • Peer Reviewed
• [Presentation] PI3K gamma is a Negative Regulator of Alloreactive CTL Induction (2013)
  • Author(s): Hideki Fujii
  • Organizer: Keystone Symposia
  • Place of Presentation: Keystone, Colorado, USA
• [Presentation] PI3K gamma is a Negative Regulator of Alloreactive CTL Induction (2013)
  • Author(s): Hideki Fujii
  • Organizer: Keystone Symposia
  • Place of Presentation: Keystone, Colorado
• [Presentation] 制御性 T 細胞機能発現における PI3K の役割 (2012)
  • Author(s): 藤猪英樹
  • Organizer: 第22回 Kyoto T Cell Conference
  • Place of Presentation: 京都
• [Presentation] Role of class IA PI3K in regulatory T cell function (2012)
  • Author(s): Hideki Fujii
  • Organizer: 99th Annual Meeting The American Association of Immunologists
  • Place of Presentation: Boston, Massachusetts USA
• [Presentation] PI3K gamma is a Negative Regulator of Alloreactive CTL Induction (2012)
  • Author(s): Atsunori Yuki
  • Organizer: 第41回 日本免疫学会総会・学術集会
  • Place of Presentation: 神戸
• [Presentation] 制御性T細胞機能発現におけるPI3Kの役割 (2012)
  • Author(s): 藤猪英樹
  • Organizer: 第21回 Kyoto T Cell Conference
  • Place of Presentation: 京都
• [Presentation] 樹状細胞におけるmTORC1はIL-10産生を介して腸管免疫に関与する (2012)
  • Author(s): 大谷真志
  • Organizer: 第21回 Kyoto T Cell Conference
  • Place of Presentation: 京都
• [Presentation] mTORC1 in dendritic cells regulates intestinal homeostasis by IL-10 (2012)
  • Author(s): Ohtani Masashi
  • Organizer: 20th International Symposium on Molecular Cell Biology of Macrophages
  • Place of Presentation: Tokyo
• [Presentation] Clarithromycin Modulates Cytokine Expression Profiles In Murine Macrophages And Dendritic Cells During Influenza A Virus Infection (2012)
  • Author(s): Makoto Wakatabe
  • Organizer: The 108th Annual International Conference of American Thoracic Society
  • Place of Presentation: San Francisco, California
• [Presentation] Modulatory Roles of Mesenchymal Stem Cell-Conditioned Media on Cytokine Expressions in Murine Macrophages and Dendritic Cells during Influenza A Virus Infection (2012)
  • Author(s): Takahiro Asami
  • Organizer: The 108th Annual International Conference of American Thoracic Society
  • Place of Presentation: San Francisco, California
• [Presentation] Role of class IA PI3K in regulatory T cell function (2012)
  • Author(s): Hideki Fujii
  • Organizer: 99th Annual Meeting The American Association of Immunologists
  • Place of Presentation: Boston, Massachusetts
• [Presentation] 制御性T細胞機能におけるclass IA PI3Kの役割 (2011)
  • Author(s): 藤猪英樹
  • Organizer: 第40回日本免疫学会学術集会(2011年11月27日-29日)
  • Place of Presentation: 幕張メッセ(千葉市)
  • Year and Date: 2011-11-29
• [Presentation] タイトジャンクションを介したランゲルハンス細胞の抗原獲得は実験的ブドウ球菌性熱傷様皮膚症候群において先制的な液性免疫を担う (2011)
  • Author(s): 永尾圭介
  • Organizer: 第40回日本免疫学会学術集会(2011年11月27日-29日)
  • Place of Presentation: 幕張メッセ(千葉市)
  • Year and Date: 2011-11-29
• [Presentation] 抑制性T細胞機能におけるPI3Kの役割 (2011)
  • Author(s): 藤猪英樹
  • Organizer: 第21回Kyoto T Cell Conference(2011年6月10日-11日)
  • Place of Presentation: 京都平安ホテル(京都市)
  • Year and Date: 2011-06-11
• [Presentation] Role of class IA PI3K in regulatory T cell function. (2011)
  • Author(s): 藤猪英樹
  • Organizer: 第40回日本免疫学会総会・学術集会
  • Place of Presentation: 千葉
• [Presentation] 抑制性T細胞機能におけるPI3Kの役割 (2011)
  • Author(s): 藤猪英樹
  • Organizer: 第21回 Kyoto T Cell Conference
  • Place of Presentation: 京都
• [Book] 免疫の事典 (2011)
  • Author(s): 桂義元、河本宏、小安重夫、山本一彦
  • Total Pages: 488
  • Publisher: 朝倉書店
• [Book] 免疫の事典 (2011)
  • Author(s): 桂義元, 他(編集)
  • Publisher: 朝倉書店