Studies on dynamics of plasmacytoid dendritic cells by gene-targeting technology.

• Project/Area Number: 22590444
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Immunology
• Research Institution: Kagawa University (2012); Osaka University (2011); The Institute of Physical and Chemical Research (2010)
• Principal Investigator: HOSHINO Katsuaki 香川大学, 医学部, 教授 (50324843)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 形質細胞様樹状細胞 / I型インターフェロン

Research Abstract

In order to track plasmacytoid dendritic cells (pDCs) in vivo, we generated SiglecH-fluorescent protein and SiglecH-cre recombinase knock-in mice, in which genes for a fluorescent protein and a cre recombinase were knocked into the gene locus of a SiglecH expressed highly in pDC. In SiglecH-fluorescent protein knock-in mice, fluorescent-positive cells were detected not only in the majority of pDC, but also in a small population of CD8+ dendritic cells in spleen and Peyer's patches. We observed all of the blood cells analyzed were tdRFP-positive in SiglecH-cre: Rosa-loxP-STOP-loxP-tdRFP mice, which carry cre-expressing tdRFP-positive cells. These data suggest that we cannot label and track pDCs in vivo by use of these knock-in mice. I propose that we choose other genes highly and specifically expressed in pDCs, and generate new knock-in mouse line to label pDCs in vivo.

Research Products

• [Journal Article] Enhanced Apoptosis by Disruption of the STAT3-I κ B-ζ Signaling Pathway in Epithelial Cells Induces Sjogren' S Syndrome-like Autoimmune Disease. (2013)
  • Author(s): 大熊敦史
  • Journal Title: Immunity Volume: 38 Issue: 3 Pages: 450-460
  • DOI: 10.1016/j.immuni.2012.11.016
  • Peer Reviewed
• [Journal Article] Spi-B is critical for plasmacytoid dendritic cell function and development (2012)
  • Author(s): Sasaki, K. Hoshino, T. Sugiyama, C. Yamazaki, T. Yano, A. Iizuka, H. Hemmi, T. Tanaka, M. Saito, M. Sugiyama, Y. Fukuda, T. Ohta, K. Sato, A. Ainai, T. Suzuki, H. Hasegawa, N. Toyama-Sorimachi, H. Kohara, T. Nagasawa, T. Kaisho
  • Journal Title: Blood Volume: 120 Issue: 24 Pages: 4733-4743
  • DOI: 10.1182/blood-2012-06-436527
  • Peer Reviewed
• [Journal Article] The Ets transcription factor Spi-B is essential for the differentiation of intestinal microfold cells (2012)
  • Author(s): Kanaya, T., Hase, K., Takahashi, D., Fukuda, S., Hoshino, K., Sasaki, I., Hemmi, H., Knoop, K. A., Kumar, N., Sato, M., Katsuno, T., Yokosuka, O., Toyooka, K., Nakai, K., Sakamoto, A., Kitahara, Y., Jinnohara, T., McSorley, S. J., Kaisho, T., Williams, I. R., and Ohno, H.
  • Journal Title: Nat Immunol Volume: 13 Issue: 8 Pages: 729-736
  • DOI: 10.1038/ni.2352
  • Peer Reviewed
• [Journal Article] CD8 陽性樹状細胞サブセットの動的制御機構の解析 (2012)
  • Author(s): 辺見弘明、山崎千尋、 星野克明、改正恒康
  • Journal Title: Cytometry Research Volume: 22 Pages: 31-35
  • NAID: 10030199789
  • URL: http://ci.nii.ac.jp/naid/10030199789
• [Journal Article] CD8陽性樹状細胞サブセットの動的制御機構の解析 (2012)
  • Author(s): 邊見弘明
  • Journal Title: Cytometry Eesearch Volume: 22 Pages: 31-35
• [Journal Article] Conservation of a chemokine system, XCR1 and its ligand, XCL1, between human and mice (2010)
  • Author(s): Yamazaki, C., Miyamoto, R., Hoshino, K., Fukuda, Y., Sasaki, I., Saito, M., Ishiguchi, H., Yano, T., Sugiyama, T., Hemmi, H., Tanaka, T., Hamada, E., Hirashima, T., Amakawa, R., Fukuhara, S., Nomura, S., Ito, T., and Kaisho, T.
  • Journal Title: Biochem Biophys Res Commun Volume: 397 Issue: 4 Pages: 756-761
  • DOI: 10.1016/j.bbrc.2010.06.029
  • Peer Reviewed
• [Journal Article] Statins, inhibitors of 3-hydroxy-3-methylglutaryl-coenzyme A reductase, function as inhibitors of cellular and molecular components involved in type I interferon production (2010)
  • Author(s): Amuro, H., Ito, T., Miyamoto, R., Sugimoto, H., Torii, Y., Son, Y., Nakamichi, N., Yamazaki, C., Hoshino, K., Kaisho, T., Ozaki, Y., Inaba, M., Amakawa, R., and Fukuhara, S.
  • Journal Title: Arthritis Rheum Volume: 62 Issue: 7 Pages: 1073-2085
  • DOI: 10.1002/art.27478
  • Peer Reviewed
• [Journal Article] Inhibitor of IkappaB kinase activity, BAY 11-7082, interferes with interferon regulatory factor 7 nuclear translocation and type I interferon production by plasmacytoid dendritic cells (2010)
  • Author(s): Miyamoto, R., Ito, T., Nomura, S., Amakawa, R., Amuro, H., Katashiba, Y., Ogata, M., Murakami, N., Shimamoto, K., Yamazaki, C., Hoshino, K., Kaisho, T., and Fukuhara, S.
  • Journal Title: Arthritis Res Ther Volume: 12 Issue: 3
  • DOI: 10.1186/ar3014
  • Peer Reviewed
• [Journal Article] Selective control of type I IFN induction by the Rac activator DOCK2 during TLR-mediated plasmacytoid dendritic cell activation (2010)
  • Author(s): Fukui Y(著者17名、17番目)
  • Journal Title: J. Exp. Med. Volume: 207 Issue: 4 Pages: 721-730
  • DOI: 10.1084/jem.20091776
  • NAID: 40019145306
  • Peer Reviewed
• [Journal Article] Cutting edge: critical role of IkappaB Kinase alpha in TLR7/9-induced type I IFN production by conventional dendritic cells (2010)
  • Author(s): Hoshino, K., Sasaki, I., Sugiyama, T., Yano, T., Yamazaki, C., Yasui, T., Kikutani, H., and Kaisho, T.
  • Journal Title: J Immunol Volume: 184 Issue: 7 Pages: 3341-3345
  • DOI: 10.4049/jimmunol.0901648
  • Peer Reviewed
• [Journal Article] Critical role of IκB Kinase α in TLR7/9-induced type I IFN production by conventional dendritic cells. (2010)
  • Author(s): Katsuaki Hoshino
  • Journal Title: J.Immunol. Volume: 184 Pages: 3341-3345
  • Peer Reviewed
• [Journal Article] Conservation of a chemokine system, XCR1 and its ligand, XCL1, between human and mice. (2010)
  • Author(s): Chihiro Yamazaki
  • Journal Title: Biochem Biophys Res Commun Volume: 397 Pages: 756-761
  • Peer Reviewed
• [Journal Article] Selective control of type I IFN induction by the Rac activator DOCK2 during TLR-mediated plasmacytoid dendritic cell activation. (2010)
  • Author(s): Kazuhito Gotoh
  • Journal Title: J.Exp.Med. Volume: 207 Pages: 721-730
  • Peer Reviewed
• [Journal Article] Statins, inhibitors of 3-hydroxy-3-methylglutaryl-coenzyme A reductase, function as inhibitors of cellular and molecular components involved in type I interferon production. (2010)
  • Author(s): Hideki Amuro
  • Journal Title: Arthritis Rheum Volume: 62 Pages: 2073-2085
  • Peer Reviewed
• [Journal Article] Inhibitor of IκB kinase activity, BAY 11-7082, interferes with interferon regulatory factor 7 nuclear translocation and type I interferon production by plasmacytoid dendritic cells. (2010)
  • Author(s): Rie Miyamoto
  • Journal Title: Arthritis Res Ther Volume: 12:R87
  • Peer Reviewed
• [Presentation] Spi-B is critical for plasmacytoid dendritic cell development in bone marrow (2012)
  • Author(s): Izumi Sasaki
  • Organizer: 第41回日本免疫学会学術集会
  • Place of Presentation: 神戸
• [Presentation] Analysis of in vivo function of XC chemokine receptor-expressing dendritic cells (2012)
  • Author(s): Masanaka Sugiyama
  • Organizer: 第41回日本免疫学会学術集会
  • Place of Presentation: 神戸
• [Presentation] Roles of an Ets family transcription factor, Spi-B, in plasmacytoid dendritic cell development in the bone marrow (2012)
  • Author(s): Izumi Sasaki
  • Organizer: International Endotoxin and Innate Immunity Society Meeting 2012
  • Place of Presentation: 東京
• [Presentation] In vivo roles of XC chemokine receptor 1-expressing dendritic cells (2012)
  • Author(s): Masanaka Sugiyama
  • Organizer: International Endotoxin and Innate Immunity Society Meeting 2012
  • Place of Presentation: 東京
• [Presentation] ABLATION OF A DENDRITIC CELL SUBSET EXPRESSING XC CHEMOKINE RECEPTOR 1 IN VIVO (2012)
  • Author(s): Hiroaki Hemmi
  • Organizer: The 12th International Symposium on Dendritic Cells (DC2012)
  • Place of Presentation: Daegu, South Korea
• [Presentation] In vivo ablation of XC chemokine receptor 1-expressing dendritic cell subset (2012)
  • Author(s): Masanaka Sugiyama
  • Organizer: The 20th International Symposium on Molecular Cell Biology of Macrophages 2012
  • Place of Presentation: 東京
• [Presentation] Roles of an Ets family transcription factor, Spi-B, in plasmacytoid dendritic cell development in the bone marrow (2012)
  • Author(s): Izumi Sasaki
  • Organizer: International Endotoxin and Innate Immunity Society Meeting 2012
  • Place of Presentation: 東京
• [Presentation] IkB-zeta-deficient Mice Develop Sjogren's syndrome-like Chronic Inflammation Caused by Dysregulation of Epitherial Homeostasis (2012)
  • Author(s): Atsushi Okuma
  • Organizer: International Endotoxin and Innate Immunity Society Meeting 2012
  • Place of Presentation: 東京
• [Presentation] IkB-zeta-deficiency in epitherial cells elicits Sjogren's syndrome-like autoimmune disease (2012)
  • Author(s): Atsushi Okuma
  • Organizer: 第41回日本免疫学会学術集会
  • Place of Presentation: 神戸
• [Presentation] Long-term memory T cell response induced by adjuvant role of iNKT cells (2012)
  • Author(s): Kanako Shimizu
  • Organizer: 第41回日本免疫学会学術集会
  • Place of Presentation: 神戸
• [Presentation] Visualization of Inflammasome Activation: Monitoring Caspase-1 Activation in Living Cells by a Genetically-encoded Probe SCAT1 (2012)
  • Author(s): Ting Liu
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
• [Presentation] BDCA3+DCの発現するケモカインレセプターXCR1とNK細胞活性化能の解析 (2011)
  • Author(s): 伊藤量基
  • Organizer: 日本免疫学会
  • Place of Presentation: 幕張メッセ(千葉県)
  • Year and Date: 2011-11-29
• [Presentation] ケモカイン受容体 Xcr1 発現樹状細胞の in vivo における機能的意義 (2011)
  • Author(s): 山崎千尋
  • Organizer: 第40回日本免疫学会学術集会
  • Place of Presentation: 幕張メッセ(千葉県)
  • Year and Date: 2011-11-28
• [Presentation] ケモカイン受容体Xcr1発現樹状細胞のin vivoにおける機能的意義 (2011)
  • Author(s): 山崎千尋
  • Organizer: 日本免疫学会
  • Place of Presentation: 幕張メッセ(千葉県)
  • Year and Date: 2011-11-28
• [Presentation] 形質細胞様樹状細胞優位に発現する Ets ファミリー転写因子 Spi-B によるI型インターフェロン遺伝子発現制御 (2011)
  • Author(s): 星野克明
  • Organizer: 新学術領域研究「自然炎症」若手ワークショップ
  • Place of Presentation: 山形県山形市
• [Presentation] Critical roles of an Ets family member, Spi-B, in pDC maturation (2010)
  • Author(s): Izumi Sasaki
  • Organizer: 14th International Congress of Immunology
  • Place of Presentation: 神戸
  • Year and Date: 2010-08-25
• [Presentation] An Ets family member, Spi-B, transactivates type I interferon promoters through the cooperation with IRF-7 (2010)
  • Author(s): Katsuaki Hoshino
  • Organizer: 14th International Congress of Immunology
  • Place of Presentation: 神戸
  • Year and Date: 2010-08-24
• [Presentation] Critical role of DOCK2 in type I IFN induction in plasmacytoid dendritic cells (2010)
  • Author(s): Kazuhito Gotoh
  • Organizer: 14th International Congress of Immunology
  • Place of Presentation: 神戸
  • Year and Date: 2010-08-23
• [Remarks] ホームページ
  • URL: http://www.med.kagawa-u.ac.jp/~immunol/index.html