| Project/Area Number |
22590521
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Shinshu University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
TERASAWA Fumiko 信州大学, 医学部, 准教授 (40109210)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2012: ¥260,000 (Direct Cost: ¥200,000、Indirect Cost: ¥60,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 臨床血液学 / フィブリノゲン / フィブリン / シトルリン化 / 関節リウマチ / 抗シトルリン化蛋白抗体 / エラスターゼ / キマーゼ / MMP-3 / 蛋白分解酵素 / プラスミン / 好中球エラスターゼ |
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| Research Abstract |
I speculated that citrullinated fibrinogen (C-Fbg) and fibrin (C-Fbn) existed in blood and joint fluid in patients with Rheumatoid Arthritis were avoided the degradation by some of proteases in the presence of anti-citrullinated protein antibody (ACPA), exerted long acting immunogenic property, and provided inflammatory region. To demonstrate my theory, I made a C-Fbg and C-Fbn, and reacted with proteases. In the presence of ACPA, C-Fbg and C-Fbn were digested similar to normal Fbg and normal Fbn, respectively, with plasmin, neutrophil elastase, tryptase, chymase, or MMP-3. In conclusion, elongation of protease digestion period for C-Fbg and C-Fbn were not observed in contrary to my expectation.
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