| Project/Area Number |
22590534
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Hokkaido University |
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Principal Investigator |
HUI Shu-ping 北海道大学, 大学院・保健科学研究院, 准教授 (90337030)
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| Co-Investigator(Kenkyū-buntansha) |
黒澤 隆夫 北海道医療大学, 薬学部, 教授 (50103198)
千葉 仁志 北海道大学, 大学院・保健科学研究院, 教授 (70197622)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 臨床化学 / 過酸化脂質 / Lipid hydroperoxide / 酸化リポ蛋白 / 免疫 / 分析化学 |
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| Research Abstract |
Cholesterylester monohydroperoxides (CEOOH), triglyceride monohydroperoxides (TGOOH, and phosphatidylcholine monohydroperoxide (PCOOH) were chemically synthesized. oxLDL was prepared by incubating native LDL from human plasma with CuSO4 for up to 4 h. The PCOOH levels during oxidation were determined and showed time course consisted of three phases. We then immunized five mice using the synthetic PCOOH and oxLDL, and antibody titers were measured. For synthetic PCOOH, the antibody titer did not show increase, but significant increase for oxLDL.
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