| Project/Area Number |
22590544
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Kanagawa Cancer Center Research Institute |
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Principal Investigator |
TSUJI Shoutaro 地方独立行政法人神奈川県立病院機構神奈川県立がんセンター(臨床研究所), がん治療学部, 主任研究員 (30285192)
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| Co-Investigator(Renkei-kenkyūsha) |
KAGEYAMA Taihei 地方独立行政法人神奈川県立病院機構神奈川県立がんセンター(臨床研究所), がん治療学部, 特別研究員 (20633473)
YAMASHITA Makiko 地方独立行政法人神奈川県立病院機構神奈 川県立がんセンター(臨床研究所), がん治療学部, 客員研究員 (60397776)
WASHIMI Kota 地方独立行政法人神奈川県立病院機構神奈 川県立がんセンター(臨床研究所), その他 部局, 医師 (30716733)
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| Project Period (FY) |
2010-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2013: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 腫瘍検査学 / 生物・生体工学 / 分子認識 / バイオテクノロジー / 遺伝子工学 / コンビナトリアルケミストリー |
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| Research Abstract |
Display technologies are procedures used for isolating target-recognizing peptides without using immunized animals. In this study, we describe a new display method, named Hishot display, that uses Escherichia coli and an expression plasmid to isolate target-recognizing peptides. This display method is based on the formation, in bacteria, of complexes between a polyhistidine (His)-tagged peptide including random sequences and the peptide-encoding mRNA including an RNA aptamer against the His-tag. Using this display method and a synthetic library of surrogate single-chain variable fragments consisting of VpreB and Ig heavy-chain variable domains, it was possible to isolate clones that could specifically recognize a particular target. The obtained clones could be purified as soluble protein produced by E. coli. The Hishot display will be a useful method to add to the repertoire of display technologies.
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