Establishment of personal identification method using a simple fluorescence APLP method for SNP testing
| Project/Area Number |
22590627
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Legal medicine
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| Research Institution | Yamagata University |
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Principal Investigator |
UMETSU Kazuo 山形大学, 医学部, 准教授 (10091828)
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| Co-Investigator(Kenkyū-buntansha) |
YUASA Isao 鳥取大学, 医学部, 准教授 (00093633)
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| Co-Investigator(Renkei-kenkyūsha) |
WATANABE Gotaro 山形県警察, 本部, 専門研究員
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | DNA鑑定 / SNP / APLP法 / 個人識別 / 総合同値確率 / 電気泳動 |
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| Research Abstract |
A total of 47 SNPs reported to be highly polymorphic in European, Chinese, Japanese, African populations (HapMap database) were selected. We developed a reliable, rapid, and economical multiplex amplified product-length polymorphism (APLP) method for analyzing the 47 SNPs. We could safely be genotyping by using four 12-multiplex PCR and subsequent 10% polyacrylamide gel electrophoresis. The amplicons ranged from 40 to 100 bp in length. The mean match probability was about 5 X 10^<-20> in all populations studied. Our APLP assay is an only two-step process that requires just mulitiplex amplification reaction and asubsequent run on native polyacrylamide gel electrophoresis.
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Report
(4 results)
Research Products
(4 results)
