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Establishment of personal identification method using a simple fluorescence APLP method for SNP testing

Research Project

Project/Area Number 22590627
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Legal medicine
Research InstitutionYamagata University

Principal Investigator

UMETSU Kazuo  山形大学, 医学部, 准教授 (10091828)

Co-Investigator(Kenkyū-buntansha) YUASA Isao  鳥取大学, 医学部, 准教授 (00093633)
Co-Investigator(Renkei-kenkyūsha) WATANABE Gotaro  山形県警察, 本部, 専門研究員
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
KeywordsDNA鑑定 / SNP / APLP法 / 個人識別 / 総合同値確率 / 電気泳動
Research Abstract

A total of 47 SNPs reported to be highly polymorphic in European, Chinese, Japanese, African populations (HapMap database) were selected. We developed a reliable, rapid, and economical multiplex amplified product-length polymorphism (APLP) method for analyzing the 47 SNPs. We could safely be genotyping by using four 12-multiplex PCR and subsequent 10% polyacrylamide gel electrophoresis. The amplicons ranged from 40 to 100 bp in length. The mean match probability was about 5 X 10^<-20> in all populations studied. Our APLP assay is an only two-step process that requires just mulitiplex amplification reaction and asubsequent run on native polyacrylamide gel electrophoresis.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (4 results)

All 2012 2011

All Presentation (4 results)

  • [Presentation] Semi-nested multiplex APLP法を用いた個人識別2012

    • Author(s)
      梅津和夫
    • Organizer
      日本法医学会
    • Place of Presentation
      アクトシティ浜松浜松市
    • Year and Date
      2012-06-08
    • Related Report
      2012 Final Research Report
  • [Presentation] Semi-nested multiplex APLP法を用いた個人識別2012

    • Author(s)
      梅津和夫
    • Organizer
      日本法医学会
    • Place of Presentation
      浜松
    • Related Report
      2012 Annual Research Report
  • [Presentation] マルチプレックス APLP法を用いた個人識別用キットの作製2011

    • Author(s)
      梅津和夫
    • Organizer
      日本法医学会
    • Place of Presentation
      コラッセふくしま福島市
    • Year and Date
      2011-06-17
    • Related Report
      2012 Final Research Report
  • [Presentation] マルチプレックスAPLP法を用いた個人識別用キットの作製2011

    • Author(s)
      梅津和夫
    • Organizer
      日本法医学会
    • Place of Presentation
      福島、コラッセ福島
    • Year and Date
      2011-06-17
    • Related Report
      2011 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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