Detecting mRNA with low expression levels on tissues using in situ RT-PCR Hybridization method: a pilot study
| Project/Area Number |
22590634
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Legal medicine
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| Research Institution | Shimane University |
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Principal Investigator |
TOGA Tomoko 島根大学, 医学部, 技術専門職員 (20403462)
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| Co-Investigator(Kenkyū-buntansha) |
TAKESHITA Haruo 島根大学, 医学部, 教授 (90292599)
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| Project Period (FY) |
2010-04-01 – 2013-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2010: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | in-situ RT-PCR / mRNA / in situ Hybridization / 川本法 / in situ RT-PCR |
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| Research Abstract |
In order to detect small-quantity mRNA on a tissue section, reverse transcription PCR was given to create cDNA, next PCR was given to target mRNA. But amplification of the target gene by PCR was not completed.
Then assumed to be stored efficiently RNA, using frozen sections fixed on the adhesive film (Kawamoto method) was applied to in situ Hybridization. Although the adhesive film could bear the protocol of in situ Hybridization in intensity, tissue sections often fall in the washing, and the dyeing residual substance remained in the film. In result, clear target's mRNA localization could not be found.
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Report
(4 results)
Research Products
(17 results)
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[Journal Article] Expression of the prolactin receptor in Japanese breast cancer2011
Author(s)
Nio Y, Iguchi C, Tsuboi K, Itakura M, Nishi T, Hashimoto K, Takamura M, Takeda H, Omori H, Koike M, Sato Y, Endo S, Ogo K, Yamaguchi K, Tsuji M, Toga T, Takeshita H
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Journal Title
Shimane Journal of Medidal Science
Volume: 28(1)
Pages: 1-6
NAID
Related Report
Peer Reviewed
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[Journal Article] Simultaneous determination of seven informative Y chromosome SNPs to differentiate East Asian, European, and African populations.2011
Author(s)
Muro T, Iida R, Fujihara J, Yasuda T, Watanabe Y, Imamura S, Nakamura H, Kimura-Kataoka K, Yuasa I, Toga T, Takeshita H.
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Journal Title
Leg Med
Volume: 13
Pages: 134-141
Related Report
Peer Reviewed
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[Presentation] Development of functional Zinc Oxide nanoparticles for biomedical applications2013
Author(s)
Y.Fujita, H.Hashimoto, M. Tongu, T.Yamada, K.Nishimura, K. Yoshikiyo, H. Akiyoshi, T. Yamamoto, A. Matsumoto, M. Fujii, J. Fujihara, T. Toga, H. Takeshita, T. Isobe
Organizer
The China-Japan Symposium on Nanomedicine
Place of Presentation
Nanjin, China
Related Report
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[Presentation] Investigation of Functional Zinc Oxide Nanoparticles for Biomedical Applications2013
Author(s)
Y. Fujita, H. Hashimoto, M. Tongu, T. Yamada, K. Nishimura, K. Yoshikiyo, H. Akiyoshi, T.Yamamoto, A. Matsumoto, M. Fujii, J. Fujihara, T. Toga, H. Takeshita, T. Isobe
Organizer
7th International Symposiumu on Nanomedicine
Place of Presentation
九州工業大学(北九州市）
Related Report
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