Establishment of biomarkers for Japanese patients with Crohn's disease

• Project/Area Number: 22590711
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: Kurume University
• Principal Investigator: MITSUYAMA Keiichi 久留米大学, 医学部, 教授 (20200066)
• Co-Investigator(Kenkyū-buntansha): YAMASAKI Hiroshi 久留米大学, 医学部, 助教 (20529565) ; KUWAKI Kotaro 久留米大学, 医学部, 助教 (60425171) ; TAKEDATSU Hidetoshi 久留米大学, 医学部, 助教 (80352144)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: クローン病 / バイオマーカー / サイトカイン / TCP353 / TCP / RCCP / ファージディスプレイ / TCPペプチド / 米由来タンパク / ELISA

Research Abstract

We isolated a novel peptide (TCP353) that specifically binds to sera from Crohn's disease (CD) patients using a phage display technique. TCP353 peptide stimulates the pro-inflammatory responses of CD mononuclear cells. An ELISA analysis for antibodies to TCP-353 demonstrated that while 61.7% of the samples from CD patients were seroreactive, seroreactivity was less common among patients with other diseases. TCP353 may have diagnostic, pathogenic and therapeutic significance with regard to the treatment of CD.

Research Products

• [Journal Article] A new therapeutic approach using a schizophyllan-based drug delivery system for inflammatory bowel disease (2012)
  • Author(s): Takedatsu H, Mitsuyama K, et al.
  • Journal Title: Mol Ther Volume: 20 Issue: 6 Pages: 1234-1241
  • DOI: 10.1038/mt.2012.24
  • Peer Reviewed
• [Journal Article] 腸内フローラを標的としたIBD治療のストラテジーと実際 (2012)
  • Author(s): 光山 慶一
  • Journal Title: IBD Research Volume: 6 Pages: 163-169
• [Journal Article] Isolation and characterization of a novel short peptide associated with Crohn's disease. (2011)
  • Author(s): Mitsuyama K,Niwa M,MasudaJ,KuwakiK,YamasakiH,Takedatsu H,KobayashiT,Sata M.
  • Journal Title: Clin Exp Immunol. Volume: 166(1) Issue: 1 Pages: 72-79
  • DOI: 10.1111/j.1365-2249.2011.04444.x
  • Peer Reviewed
• [Journal Article] Isolation and characterization of a novel short peptide associated with Crohn's disease (2011)
  • Author(s): Mitsuyama K, Niwa M, Masuda J, Kuwaki K, Yamasaki H, Takedatsu H, Kobayashi T, Sata M.
  • Journal Title: Clin Exp Immunol Volume: １６６ Pages: 72-79
  • Peer Reviewed
• [Journal Article] IBDとバイオマーカー:血清抗体の意義. (2010)
  • Author(s): 光山慶一,佐田通夫.
  • Journal Title: IBD Research. Volume: 4(4) Pages: 274-281
• [Journal Article] IBDとバイオマーカー・血性抗体の意義 (2010)
  • Author(s): 光山慶一、佐田通夫
  • Journal Title: IBD Research Volume: 4 Pages: 274-281
• [Presentation] クローン病の新規バイオマーカーの検討 (2010)
  • Author(s): 光山慶一、増田淳也、山崎博、桑木光太郎、竹田津英稔、吉岡慎一郎、小林哲平、鶴田修、佐田通夫
  • Organizer: 第96回日本消化器病学会総会
  • Place of Presentation: 新潟