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The role of microtubules structure in hepatitis C virus replicationand the possibility of its target molecule for anti-HCV agent

Research Project

Project/Area Number 22590724
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Gastroenterology
Research InstitutionUniversity of Yamanashi

Principal Investigator

YAMASHITA Atsuya  山梨大学, 大学院・医学工学総合研究部, 助教 (00334871)

Co-Investigator(Renkei-kenkyūsha) ENOMOTO Nobuyuki  山梨大学, 大学院・医学工学総合研究部, 教授 (20251530)
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords肝臓学 / C型肝炎ウイルス / 慢性C型肝炎治療薬 / ウイルス / 内科 / 治療薬
Research Abstract

We previously found that the antifungal agent, Griseofulvin, hasa suppressive effect on HCV replication. On the basis of this result, we optimized thestructure of Griseofulvin for searching a new candidate compound of an anti-HCV agentamong Griseofulvin analogues, and analyzed molecular mode of action of Griseofulvinagainst HCV replication. Consequently, we found two Griseofulvin analogues were moreeffective in suppressing HCV replication than Griseofulvin. However, we were unable toclarify molecular mode of action of Griseofulvin against HCV replication.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (50 results)

All 2013 2012 2011 2010 Other

All Journal Article (15 results) (of which Peer Reviewed: 14 results) Presentation (31 results) Remarks (4 results)

  • [Journal Article] Cholesterol sulfate as a potentialinhibitor of hepatitis C virus NS3helicase.2013

    • Author(s)
      Furuta A, Yamashita A, et al
    • Journal Title

      J Enzyme Inhib Med Chem

      Volume: (in press)

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Psammaplin A inhibits hepatitis C virusNS3 helicase.2013

    • Author(s)
      Salam KA, Yamashita A, et al.
    • Journal Title

      J Nat Med.

      Volume: (in press)

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Serum RANTES level influences the responseto pegylated interferon and ribavirintherapy in chronic hepatitis C2013

    • Author(s)
      Komase K, Yamashita A, Enomoto N, et al
    • Journal Title

      Hepatol Res

      Volume: (in press)

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] IL-28B (IFN-λ3) and IFN-αsynergistically inhibit Shindo H HCVreplication.2013

    • Author(s)
      Shindo H, Yamashita A, Enomoto N, et al
    • Journal Title

      J Viral Hepat

      Volume: Vol.20, No.4 Pages: 281-9

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Antimicrobial peptide LL-37 produced byHSV-2-infected keratinocytes enhances HIVinfection of Langerhans cells2013

    • Author(s)
      Ogawa Y, Yamashita A, et al.
    • Journal Title

      Cell Host Microbe.

      Volume: Vol.13,No.1 Pages: 77-86

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Antimicrobial peptide LL-37 produced by HSV-2-infected keratinocytes enhances HIV infection of Langerhans cells2013

    • Author(s)
      Ogawa Y, Kawamura T, Matsuzawa T, Aoki R, Gee P, Yamashita A, Moriishi K, Yamasaki K, Koyanagi Y. Blauvelt A, Shimada S.
    • Journal Title

      Cell Host and Microbe

      Volume: 13 Issue: 1 Pages: 77-86

    • DOI

      10.1016/j.chom.2012.12.002

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] IL-28B (IFN-λ3) and IFN-α synergistically inhibit HCV replication.2013

    • Author(s)
      Shindo H
    • Journal Title

      J Viral Hepat.

      Volume: 20 Issue: 4 Pages: 281-289

    • DOI

      10.1111/j.1365-2893.2012.01649.x

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Inhibition of both protease and helicaseactivities of hepatitis C virus NS3 by anethyl acetate extract of marine spongeAmphimedon sp.2012

    • Author(s)
      Fujimoto Y, Yamashita A, Enomoto N, etal.
    • Journal Title

      PLoS One

      Volume: Vol.7

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Inhibition of hepatitis C virusreplication and viral helicase by ethylacetate extract of the marine feather starAlloeocomatella polycladia.2012

    • Author(s)
      Yamashita A, Enomoto N, et al.
    • Journal Title

      Mar Drugs.

      Volume: Vol.10,No.4 Pages: 744-61

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Inhibition of hepatitis C virus NS3helicase by manoalide.2012

    • Author(s)
      Salam KA, Yamashita A, et al.
    • Journal Title

      J Nat Prod.

      Volume: Vol.75, No.4 Pages: 650-4

    • Related Report
      2012 Final Research Report
  • [Journal Article] Inhibition of hepatitis C Virus NS3 helicase by manoalide2012

    • Author(s)
      Salam, K.A.
    • Journal Title

      J.Nat.Prod.

      Volume: (in press) Issue: 4 Pages: 650-654

    • DOI

      10.1021/np200883s

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Inhibition of Hepatitis C Virus Replication and Viral Helicase by Ethyl Acetate Extract of the Marine Feather Star Alloeocomatella polycladia2012

    • Author(s)
      Yamashita A, Maekawa S, et al
    • Journal Title

      Mar.Drugs

      Volume: 10 Issue: 4 Pages: 744-761

    • DOI

      10.3390/md10040744

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Inhibition of both protease and helicase activities of hepatitis C virus NS3 by an ethyl acetate extract of marine sponge Amphimedon sp.2012

    • Author(s)
      Fujimoto Y
    • Journal Title

      PLoS One.

      Volume: 7 Issue: 11 Pages: 744-761

    • DOI

      10.1371/journal.pone.0048685

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A new method for induced fit docking(GENIUS) and its application to virtualscreening of novel HCV NS3-4A proteaseinhibitors2011

    • Author(s)
      Takaya D, Yamashita A, Enomoto N, et al.
    • Journal Title

      Bioorg Med Chem

      Volume: Vol.19,No.22 Pages: 6892-905

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] A new method for induced fit docking (GENIUS) and its application to virtual screening of novel HCV NS3-4A protease inhibitors2011

    • Author(s)
      Takaya D, Sakamoto N, et al.
    • Journal Title

      Bioorganic & Medicinal Chemistry

      Volume: 19(22) Issue: 22 Pages: 6892-6905

    • DOI

      10.1016/j.bmc.2011.09.023

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Presentation] HCV複製増殖に関わる新規宿主因子FKBP6:FKBP6 は NS5A と結合し HCV 複製を制御する2012

    • Author(s)
      葛西 宏威
    • Organizer
      第35回日本分子生物学会
    • Place of Presentation
      福岡国際会議場・マリンメッセ福岡(福岡市)
    • Year and Date
      2012-12-13
    • Related Report
      2012 Final Research Report
  • [Presentation] メキシコ先住民族における HBV 感染および遺伝子型の分析(第2報)2012

    • Author(s)
      後藤 才郎
    • Organizer
      第35回日本分子生物学会
    • Place of Presentation
      福岡国際会議場・マリンメッセ福岡(福岡市)
    • Year and Date
      2012-12-12
    • Related Report
      2012 Final Research Report
  • [Presentation] Caffeic acid phenethyl ester とその類縁化合物による HCV ゲノム複製阻害効果の検討2012

    • Author(s)
      山下 篤哉
    • Organizer
      第60回日本ウイルス学会
    • Place of Presentation
      グランキューブ大阪(大阪市)
    • Year and Date
      2012-11-14
    • Related Report
      2012 Final Research Report
  • [Presentation] 海綿動物 Amphimedon sp.抽出画分による HCVNS3 プロテアーゼ・ヘリカーゼ活性阻害の解析2012

    • Author(s)
      藤本 雄介
    • Organizer
      第60回日本ウイルス学会
    • Place of Presentation
      グランキューブ大阪(大阪市)
    • Year and Date
      2012-11-14
    • Related Report
      2012 Final Research Report
  • [Presentation] 新規宿主因子 FKBP6 による HCV 複製制御機構の解析2012

    • Author(s)
      葛西 宏威
    • Organizer
      第60回日本ウイルス学会
    • Place of Presentation
      グランキューブ大阪(大阪市)
    • Year and Date
      2012-11-13
    • Related Report
      2012 Final Research Report
  • [Presentation] 海洋生物抽出物より取得した複数種の硫酸化合物による C 型肝炎ウイルス NS3 helicase阻害作用2012

    • Author(s)
      古田 篤史
    • Organizer
      第64回日本生物工学会大会
    • Place of Presentation
      神戸国際会議場(神戸市)
    • Year and Date
      2012-10-25
    • Related Report
      2012 Final Research Report
  • [Presentation] Inhibition of hepatitis C virusreplication and viral helicase by ethylacetate extract of the marine feather starAlloeocomatella polycladia2012

    • Author(s)
      Atsuya Yamashita
    • Organizer
      25th International Conference onAntiviral Research
    • Place of Presentation
      RoytonSapporo (Sapporo, Japan)
    • Year and Date
      2012-04-16
    • Related Report
      2012 Final Research Report
  • [Presentation] Caffeic acid phenethyl esterとその類縁化合物によるHCVゲノム複製阻害効果の検討2012

    • Author(s)
      山下 篤哉
    • Organizer
      第60回日本ウイルス学会
    • Place of Presentation
      グランキューブ大阪(大阪市)
    • Related Report
      2012 Annual Research Report
  • [Presentation] HCV NS3 プロテアーゼ・ヘリカーゼ活性阻害の解析2012

    • Author(s)
      藤本 雄介
    • Organizer
      第60回日本ウイルス学会
    • Place of Presentation
      グランキューブ大阪(大阪市)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 新規宿主因子FKBP6によるHCV複製制御機構の解析2012

    • Author(s)
      葛西 宏威
    • Organizer
      第60回日本ウイルス学会
    • Place of Presentation
      グランキューブ大阪(大阪市)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 海洋生物抽出物より取得した複数種の硫酸化合物によるC型肝炎ウイルスNS3 helicase阻害作用2012

    • Author(s)
      古田 篤史
    • Organizer
      第64回日本生物工学会大会
    • Place of Presentation
      神戸国際会議場(神戸市)
    • Related Report
      2012 Annual Research Report
  • [Presentation] メキシコ先住民族におけるHBV感染および遺伝子型の分析(第2報)2012

    • Author(s)
      後藤 才郎
    • Organizer
      第35回日本分子生物学会
    • Place of Presentation
      福岡国際会議場・マリンメッセ福岡 (福岡市)
    • Related Report
      2012 Annual Research Report
  • [Presentation] HCV複製増殖に関わる新規宿主因子FKBP6:FKBP6はNS5Aと結合しHCV複製を制御する2012

    • Author(s)
      葛西 宏威
    • Organizer
      第35回日本分子生物学会
    • Place of Presentation
      福岡国際会議場・マリンメッセ福岡 (福岡市)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Inhibition of hepatitis C virus replication and viral helicase by ethyl acetate extract of the marine feather star Alloeocomatella polycladia.2012

    • Author(s)
      Atsuya Yamashita
    • Organizer
      25th International Conference on Antiviral Research
    • Place of Presentation
      Royton Sapporo (Sapporo, Japan)
    • Related Report
      2012 Annual Research Report
  • [Presentation] メキシコ先住民における HBV 感染および遺伝型の分析2011

    • Author(s)
      照沼 修
    • Organizer
      第34回日本分子生物学会
    • Place of Presentation
      パシフィコ横浜(横浜市)
    • Year and Date
      2011-12-14
    • Related Report
      2012 Final Research Report
  • [Presentation] Regulation of HCV replication byFKBP8-dependent or -independentHsp90activity2011

    • Author(s)
      Kunihiro Kawakami
    • Organizer
      第34回日本分子生物学会
    • Place of Presentation
      パシフィコ横浜(横浜市)
    • Year and Date
      2011-12-14
    • Related Report
      2012 Final Research Report
  • [Presentation] メキシコ先住民におけるHBV感染および遺伝型の分析2011

    • Author(s)
      照沼修
    • Organizer
      第34回日本分子生物学会
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Year and Date
      2011-12-14
    • Related Report
      2011 Annual Research Report
  • [Presentation] Regulation of HCV replication by FKBP8-dependent or-independent Hsp90 activity2011

    • Author(s)
      Kunihiro Kawakami
    • Organizer
      第34回日本分子生物学会
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Year and Date
      2011-12-14
    • Related Report
      2011 Annual Research Report
  • [Presentation] 海洋生物抽出物より取得した cholesterolsulfate によるC型肝炎ウイルスNS3helicase 阻害作用2011

    • Author(s)
      古田 篤史
    • Organizer
      第63回日本生物工学会大会
    • Place of Presentation
      東京農工大(東京都)
    • Year and Date
      2011-09-27
    • Related Report
      2012 Final Research Report
  • [Presentation] 海洋生物抽出物より取得したcholesterolsulfateによるC型肝炎ウイルスNS3helicase 阻害作用2011

    • Author(s)
      古田 篤史
    • Organizer
      第63回日本生物工学会大会
    • Place of Presentation
      東京農工大(東京都)
    • Year and Date
      2011-09-27
    • Related Report
      2012 Final Research Report
  • [Presentation] 海洋生物抽出物より取得したcholesterol sulfateによるC型肝炎ウイルスNS3 helicase阻害作用2011

    • Author(s)
      古田篤史
    • Organizer
      第63回日本生物工学会大会
    • Place of Presentation
      東京農工大(東京都)
    • Year and Date
      2011-09-27
    • Related Report
      2011 Annual Research Report
  • [Presentation] Marine natural products as a source of thenovel antiviral agent targeting to HCV NS3helicase2011

    • Author(s)
      Atsuya Yamashita
    • Organizer
      International Union of MicrobiologicalSocieties 2011 Congress
    • Place of Presentation
      Sapporo Convention Center (Sapporo,Japan)
    • Year and Date
      2011-09-15
    • Related Report
      2012 Final Research Report
  • [Presentation] Inhibitory effect of marine naturalproducts on the replication of hepatitisC virus.2011

    • Author(s)
      Yuusuke Fujimoto
    • Organizer
      International Union of MicrobiologicalSocieties 2011 Congress
    • Place of Presentation
      Sapporo Convention Center (Sapporo,Japan)
    • Year and Date
      2011-09-15
    • Related Report
      2012 Final Research Report
  • [Presentation] Marine natural products as a source of the novel antiviral agent targeting to HCV NS3 helicase2011

    • Author(s)
      Atsuya Yamashita
    • Organizer
      International Union of Microbiological Societies 2011 Congress
    • Place of Presentation
      Sapporo Convention Center (Sapporo, Japan)
    • Year and Date
      2011-09-15
    • Related Report
      2011 Annual Research Report
  • [Presentation] Inhibitory effect of marine natural products on the replication of hepatitis C virus2011

    • Author(s)
      Yuusuke Fujimoto
    • Organizer
      International Union of Microbiological Societies 2011 Congress
    • Place of Presentation
      Sapporo Convention Center (Sapporo, Japan)
    • Year and Date
      2011-09-15
    • Related Report
      2011 Annual Research Report
  • [Presentation] In silico分子設計手法によるHCV NS3protease活性阻害化合物の創製2011

    • Author(s)
      山下 篤哉
    • Organizer
      第21回抗ウイルス療法研究会
    • Place of Presentation
      金沢市文化ホール(金沢市)
    • Year and Date
      2011-05-29
    • Related Report
      2012 Final Research Report
  • [Presentation] 海洋生物をライブラリーソースとした HCVNS3 Helicase 活性阻害化合物の検索2011

    • Author(s)
      山下 篤哉
    • Organizer
      第21回抗ウイルス療法研究会
    • Place of Presentation
      金沢市文化ホール(金沢市)
    • Year and Date
      2011-05-29
    • Related Report
      2012 Final Research Report
  • [Presentation] 海洋生物をライブラリーソースとしたHCV NS3 Helicase活性阻害化合物の検索2011

    • Author(s)
      山下篤哉
    • Organizer
      第21回抗ウイルス療法研究会
    • Place of Presentation
      金沢市文化ホール(石川県)
    • Year and Date
      2011-05-29
    • Related Report
      2011 Annual Research Report
  • [Presentation] In silico分子設計手法によるHCV NS3 protease活性阻害化合物の創製2011

    • Author(s)
      山下篤哉
    • Organizer
      第21回抗ウイルス療法研究会
    • Place of Presentation
      金沢市文化ホール(石川県)
    • Year and Date
      2011-05-29
    • Related Report
      2011 Annual Research Report
  • [Presentation] 抗HCV NS2 タンパク活性阻害剤High throughput screeningのためのHCV subgenomic replicon細胞の構築2010

    • Author(s)
      藤本雄介
    • Organizer
      第58回日本ウイルス学会
    • Place of Presentation
      あわぎんホール(徳島県徳島市)
    • Year and Date
      2010-11-07
    • Related Report
      2010 Annual Research Report
  • [Presentation] 沖縄産ウミシダ(Alloeocomatela polycladia)抽出物の抗HCV NS3 helicase阻害活性によるHCV増殖抑制効果2010

    • Author(s)
      山下篤哉
    • Organizer
      第58回日本ウイルス学会
    • Place of Presentation
      あわぎんホール(徳島県徳島市)
    • Year and Date
      2010-11-07
    • Related Report
      2010 Annual Research Report
  • [Remarks] 山梨大学研究者総覧

    • URL

      http://erdb.yamanashi.ac.jp/rdb/A_DispInfo.Scholar/0/E88A6696C4043E75.html

    • Related Report
      2012 Final Research Report
  • [Remarks] 山梨大学大学院・医学工学総合研究部・医学学域・微生物学講座

    • URL

      http://www.med.yamanashi.ac.jp/clinical_basic/microbio/Microbiology_Yamanashi_Uni_Japanese/Home.html

    • Related Report
      2012 Final Research Report
  • [Remarks] 山梨大学研究者総覧

    • URL

      http://erdb.yamanashi.ac.jp/rdb/A_DispInfo.Scholar/0/E88A6696C4043E75.html

    • Related Report
      2012 Annual Research Report
  • [Remarks] 山梨大学大学院・医学工学総合研究部・医学学域・微生物学講座

    • URL

      http://www.med.yamanashi.ac.jp/clinical_basic/microbio/Microbiology_Yamanashi_Uni_Japanese/Home.html

    • Related Report
      2012 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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