The development of method for liver fibrosis inhibition by controlling liver fibrosis

• Project/Area Number: 22590727
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: Osaka City University (2012); Kyoto University (2010-2011)
• Principal Investigator: MURAKAMI Yoshiki 大阪市立大学, 大学院・医学研究科, 病院講師 (00397556)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
• Keywords: マイクロ RNA / 肝線維化 / 肝発癌 / miRNA / マイクロRNA / 慢性C型肝炎 / 肝細胞癌 / エクソソーム / C型肝炎ウイルス

Research Abstract

Liver fibrosis in chronic liver disease was progressed without suitable medical treatment. We clarified the microRNA (miRNA) of which aberrant expression was related to the progression of liver fibrosis by using microarray analysis in liver

Research Products

• [Journal Article] The expression level of miR-18b in hepatocellular carcinoma is associated with the grade of malignancy and prognosis BMC Cancer (2013)
  • Author(s): Murakami Y, Tamori A, Itami S, Tanahashi T, Toyoda H, Tanaka M, Wu W, Brojigin N, Kaneoka Y, Maeda A,Kumada T, Kawada N, Kubo S and Kuroda M
  • Volume: 13
  • Pages: 99-99
• [Journal Article] Higher Hepatic Gene Expression and Serum Levels of Matrix Metalloproteinase-2 are Associated with Steatohepatitis in Non-alcoholic Fatty Liver Diseases (2013)
  • Author(s): Toyoda H, Kumada T, Kiriyama T, Tanigawa M, Hisanaga Y, Kanamori A, Tada Y, and Murakami Y
  • Journal Title: Biomarkers Volume: 18 Pages: 82-7
• [Journal Article] Association between Hepatic Steatosis and Hepatic Expression of Genes Involved in Innate Immunity in Patients with Chronic Hepatitis C (2013)
  • Author(s): Toyoda H, Kumada T, Kiriyama S, Tanikawa M, Hisanaga Y, Kanamori A, Tada T, Kitabatake S, Murakami Y.
  • Journal Title: cytokine (in press) Volume: in press
  • Peer Reviewed
• [Journal Article] A Phase I study investigating the safety and pharmacokinetics of highly bioavailable curcumin in cancer patients. (2013)
  • Author(s): Kanai M,Otsuka Y, Otsuka K, Sato M, Nishimura T, Mori Y, Kawaguchi M, Hatano E, Kodama Y, Matsumoto S, Murakami Y, Imaizumi A, Chiba T, Nishihira J, Shibata H.
  • Journal Title: Cancer Chemotherapy and Pharmacology (in press) Volume: in press
  • Peer Reviewed
• [Journal Article] The expression level of miR-18b in hepatocellular carcinoma is associated with the grade of malignancy and prognosis (2013)
  • Author(s): Murakami Y, Tamori A, Itami S, Tanahashi T, Toyoda H, Tanaka M, Wu W, Brojigin N, Kaneoka Y, Maeda A, Kumada T, Kawada N, Kubo S and Kuroda M.
  • Journal Title: BMC Cancer Volume: 13 Issue: 1 Pages: 99-99
  • DOI: 10.1186/1471-2407-13-99
  • Peer Reviewed
• [Journal Article] Higher Hepatic Gene Expression and Serum Levels of Matrix Metalloproteinase-2 are Associated with Steatohepatitis in Non-alcoholic Fatty Liver Diseases. (2013)
  • Author(s): Toyoda H, Kumada T, Kiriyama T, Tanigawa M, Hisanaga Y, Kanamori A, Tada Y, and Murakami Y.
  • Journal Title: Biomarkers Volume: 18 Issue: 1 Pages: 82-87
  • DOI: 10.3109/1354750x.2012.738249
  • Peer Reviewed
• [Journal Article] Comprehensive miRNA expression analysis in peripheral blood can diagnose liver disease (2012)
  • Author(s): Murakami Y, Toyoda H, Tanahashi T, Tanaka J, Kumada T, Yoshioka Y, Kosaka N, Ochiya T, and Y-h Taguchi
  • Journal Title: PLoS ON Volume: 7
• [Journal Article] Comprehensive miRNA expression analysis in peripheral blood can diagnose liver disease. (2012)
  • Author(s): Murakami Y, Toyoda H, Tanahashi T, Tanaka J, KumadaT, Yoshioka Y, Kosaka N, OchiyaT, Taguchi YH.
  • Journal Title: PLoS ONE Volume: 7(10) Issue: 10 Pages: e48366-e48366
  • DOI: 10.1371/journal.pone.0048366
  • Peer Reviewed
• [Journal Article] Role of miR-19b and its target mRNAs in 5-fluorouracil resistance in colon cancer cells (2012)
  • Author(s): Kurokawa K, Tanahashi T, Iima T, Yamamoto Y, Akaike Y, Nishida K, Masuda K, Kuwano Y, Murakami Y, Fukushima M, Rokutan
  • Journal Title: J Gastroenterol Volume: 47 Issue: 8 Pages: 883-895
  • DOI: 10.1007/s00535-012-0547-6
  • NAID: 10031051201
  • Peer Reviewed
• [Journal Article] Dysregulation of IFN system can lead to poor response to pegylated interferon and ribavirin therapy in chronic hepatitis C (2011)
  • Author(s): Onomoto K, Morimoto S, Kawaguchi T, Toyoda H, Tanaka M, Kuroda M, Uno K, Kumada T, Matsuda F, Shimotohno K, Fujita T, and Murakami Y
  • Journal Title: PLoS One Volume: 6
  • NAID: 120003072107
• [Journal Article] Overexpression of miR-199 and 200 families is associated with the progression of liver fibrosis (2011)
  • Author(s): Murakami Y, Toyoda H, Tanaka M, Kuroda M, Harada Y, Matsuda F, Tajima A, Kosaka N, Ochiya T, Shimotohno K
  • Journal Title: PLoS One Volume: 6
• [Journal Article] Dysregulation of IFN system can lead to poor response to pegylated interferon and ribavirin therapy in chronic hepatitis C (2011)
  • Author(s): Onomoto K, et al
  • Journal Title: PLoS ONE Volume: 6
  • Peer Reviewed
• [Journal Article] Overexpression of miR-199 and 200 families is associated with the progression of liver fihrosis (2011)
  • Author(s): Murakami Y, et al
  • Journal Title: PLoS ONE Volume: 6
  • Peer Reviewed
• [Journal Article] Overexpression of miR-199 and 200 families is associated with the progression of liver fibrosis. (2011)
  • Author(s): Murakami Y, et al.
  • Journal Title: PLoS ONE Volume: 6
  • Peer Reviewed
• [Journal Article] Hepatic microRNA expression is associated with the response to interferon treatment of chronic hepatitis C (2010)
  • Author(s): Murakami Y, Tanaka M, Toyoda H, Hayashi K, Kuroda M, Tajima A, and Shimotohno K
  • Journal Title: BMC Medical Genomics Volume: 3 Pages: 48-48
  • NAID: 120004770254
• [Journal Article] Polyubiquitin conjugation to NEMO by TRIM23 is critical in antiviral defense (2010)
  • Author(s): Arimoto K, Fumani K, Saeki Y, Tanaka K, Okawa K, Takeuchi O, Akira S, Murakami Y, Shimotohno K
  • Journal Title: PNA Volume: 107 Pages: 15856-61
• [Journal Article] Deregulation of miR-92a expression is implicated in hepatocellular carcinoma development (2010)
  • Author(s): Shigoka M, Tsuchida A, Matsudo T, Nagakawa Y, Saito S, Suzuki Y, Aoki T, Murakami Y, Toyoda H, Kumada T, Bartenschlager R, Kato N, Ikeda M, Takashina T, Tanaka M, Suzuki R, Oikawa K, Takanashi M, Kuroda M
  • Journal Title: Pathol Int Volume: 60 Pages: 351-357
  • NAID: 10029331782
• [Journal Article] Hepatic microRNA expression is associated with the response to interferon treatment of chronic hepatitis C. (2010)
  • Author(s): Murakami Y, et al.
  • Journal Title: BMC Med Genomics Volume: 3 Pages: 48-48
  • NAID: 120004770254
  • Peer Reviewed
• [Journal Article] Deregulation of miR-92a expression is implicated in hepatocellular car cinoma development. (2010)
  • Author(s): Shigoka M, et al.
  • Journal Title: Pathol Int Volume: 60 Pages: 351-355
  • Peer Reviewed
• [Journal Article] Association between Hepatic Steatosis and Hepatic Expression of Genes Involved in Innate Immunity in Patients with Chronic Hepatitis C
  • Author(s): Toyoda H, Kumada T, Kiriyama S, Tanikawa M, Hisanaga Y, Kanamori A, Tada T, Kitabatake S, Murakami Y
  • Journal Title: Cytokine Volume: (in press)
• [Presentation] 肝疾患における肝線維化バイオマーカーとしてのmiR-222の有用性 (2013)
  • Author(s): 河田則文、小川智弘、榎本大、藤井英樹、村上善基、吉里勝利、池田一雄
  • Organizer: 第50回日本臨床分子医学会
  • Place of Presentation: 東京国際フォーラム
• [Presentation] 肝細胞癌に関係するmiRNAを使った肝癌遺伝子治療開発の試み (2013)
  • Author(s): 村上善基、河田則文、田口善弘
  • Organizer: 第50回日本臨床分子医学会
  • Place of Presentation: 東京国際フォーラム
• [Presentation] Expression level of miR-18b can diagnose the prognosis and malignancy of hepatocellular carcinoma (2012)
  • Author(s): Murakami Y, Tamori A, Kubo S, Kawada N
  • Organizer: 第71回日本癌学会学術総
  • Place of Presentation: 札幌市
  • Year and Date: 2012-09-20
• [Presentation] Expression level of miR-18b can diagnose the prognosis and malignancy of hepatocellular carcinoma. (2012)
  • Author(s): Murakami Y, Tamori A, Kubo S, Kawada N.
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: 札幌市教育文化会館
• [Presentation] インターフェロン関連遺伝子発現プロファイルは慢性 C 型肝炎治療効果予測に有用なマーカーである (2011)
  • Author(s): 村上善基、豊田秀徳、熊田卓
  • Organizer: 第15回日本肝臓学会大会
  • Place of Presentation: 福岡市
  • Year and Date: 2011-10-20
• [Presentation] インターフェロン関連遺伝子発現プロファイルは慢性C型肝炎治療効果予測に有用なマーカーである (2011)
  • Author(s): 村上善基, 他
  • Organizer: 第15回日本肝臓学会大会
  • Place of Presentation: 福岡市
  • Year and Date: 2011-10-20
• [Presentation] マイクロ RNA 発現プロファイルは慢性肝疾患の病態評価に有用なマーカーである (2011)
  • Author(s): 村上善基、豊田秀徳、熊田卓
  • Organizer: 第97回日本消化器病学会総会
  • Place of Presentation: 東京都
  • Year and Date: 2011-05-15
• [Presentation] Aberrant expression of miR-199 and 200 families is associated the progression of liver fibrosis (2010)
  • Author(s): Murakami Y, Tanaka M, Kuroda M, Kosaka N, Ochiya T, Shimotohno K
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪市
  • Year and Date: 2010-09-22
• [Presentation] Over expression of miR-199 and 200 families is associated to the progression of liver fibrosis (2010)
  • Author(s): 村上善基
  • Organizer: 日本癌学会学術集会
  • Place of Presentation: 大阪市
  • Year and Date: 2010-09-22
• [Presentation] インターフェロン関連遺伝子発現パターンを利用した慢性 C 型肝炎治療効果予測 (2010)
  • Author(s): 村上善基
  • Organizer: 第75回日本インターフェロンサイトカイン学会 シンポジウム
  • Place of Presentation: 北九州市
  • Year and Date: 2010-06-25
• [Presentation] マイクロ RNA 発現プロファイルを用いた慢性 C 型肝炎ウイルス治療効果予測 (2009)
  • Author(s): 村上善基、豊田秀徳、下遠野邦忠
  • Organizer: 第13回日本肝臓学会大会
  • Place of Presentation: 京都市
  • Year and Date: 2009-10-15
• [Patent(Industrial Property Rights)] Association between Hepatic Steatosis and Hepatic Expression of Genes Involved in Innate Immunity in Patients with Chronic Hepatitis C (2013)
  • Inventor(s): 村上善基
  • Industrial Property Rights Holder: 村上善基
  • Industrial Property Rights Type: 特許
  • Filing Date: 2013-02-23
  • Overseas
• [Patent(Industrial Property Rights)] 遺伝子発現解析を用いた肝線維化の評価方法 (2010)
  • Inventor(s): 村上善基
  • Industrial Property Rights Holder: 村上善基京都大学
  • Filing Date: 2010-04-05