Functional analysis of AIF molecule and developement of therapy of rheumatoid arthritis

• Project/Area Number: 22591080
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: 膠原病・アレルギー・感染症内科学
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: KAWAHITO Yutaka 京都府立医科大学, 医学研究科, 准教授 (50336731)
• Co-Investigator(Kenkyū-buntansha): KOHNO Masataka 京都府立医科大学, 医学研究科, 助教 (60405256) ; NAITO Yuji 京都府立医科大学, 医学研究科, 准教授 (00305575) ; OHTA Mitsuhiro 神戸薬科大学, 薬学部, 薬学部 (00330423)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 関節リウマチ / AIF-1 / ケモカイン / サイトカイン / IL-6 / AIF / ケモタキシス

Research Abstract

Functional analysis of AIF molecule and developement of therapy of rheumatoid arthritisAllograft inflammatory factor (AIF)-1 has been identified in chronic rejection of rat cardiac allografts and is thought to be involved in the immune response, however its molecular function is not fully understood. In this study , we compared mRNA expression profile of rhAIF-1 stimulated peripheral blood mononuclear cells by using the high-density oligonucleotide microarray technique. As biological activity in mononuclear cells of AIF-1, we indicated that AIF-1 induced IL-6 and many chemokines leading to chemotaxis and tissue fibrosis.

Research Products

• [Journal Article] Allograft inflammatory factor-1 is overexpressed and induces fibroblast chemotaxis in the skin of sclerodermatous GVHD in a murine model. (2011)
  • Author(s): Yamamoto A, Ashihara E, Nakagawa Y,Obayashi H, Ohta M, Hara H, Adachi T, Seno T, Kadoya M, Hamaguchi M, Ishino H, Kohno M, Maekawa T, Kawahito Y
  • Journal Title: Immunology Letters Volume: 135 Pages: 144-150
  • Peer Reviewed
• [Presentation] 関節リウマチにおけるAllograft inflammatory factor-1のサイトカイン・ケモカイン産生能および細胞遊走能についての検討 (2012)
  • Author(s): 山本相浩、河野正孝、茎田祐司、藤岡数記、永原秀剛、村上 憲、藤井 渉、中村 薫、妹尾高宏、角谷昌俊、 川人 豊
  • Organizer: 第40回日本臨床免疫学会
  • Place of Presentation: 東京
  • Year and Date: 2012-09-27
• [Presentation] Allograft inflammatory factor-1 is related with the pathogenesis of skin fibrosis in a murine model of fibromatous GVHD (2011)
  • Author(s): Yamamoto A, Ashihara E, Obayashi H, Seno T, Kadoya M, Hamaguchi M, Ishino H, Tsubouchi Y, Kohno M, Maekawa T, Kawahito Y
  • Organizer: The 74st annual meeting of the American College of Rheumatology
  • Place of Presentation: Atlanta, USA
  • Year and Date: 2011-11-08
• [Presentation] Allograft inflammatory factor-1 is related with the pathogenesis of skin fibrosis in a murine model of fibromatous GVHD. (2011)
  • Author(s): Yamamoto A, Ashihara E, Obayashi H, Seno T, Kadoya M, Hamaguchi M, Ishino H, Tsubouchi Y, Kohno M, Maekawa T, Kawahito Y.
  • Organizer: The 74st annual meeting of the American College of Rheumatology
  • Place of Presentation: Atlanta, USA
  • Year and Date: 2011-11-08
• [Presentation] Allograft inflammatory factor-1 is related with the pathogenesis of skin fibrosis in a murine model of fibromatous GVHD (2010)
  • Author(s): Yamamoto A, Ashihara E, Obayashi H, Seno T, Kadoya M, Hamaguchi M, Ishino H, Tsubouchi Y, Kohno M, Maekawa T, Kawahito Y
  • Organizer: The annual European League Against Rheumatism
  • Place of Presentation: Roma, Italy
  • Year and Date: 2010-06-18
• [Presentation] サイトカイン病態への作用 強皮症様 GVHD モデルの皮膚における AIF-1の関与についての検討 (2010)
  • Author(s): 山本相浩、芦原英司、川人 豊、河野正孝、坪内康則、石野秀岳、濱口真英、角谷昌俊、妹尾高宏
  • Organizer: 第54回日本リウマチ学会総会・学術集会
  • Place of Presentation: 神戸
• [Presentation] Allograft inflammatory factor-1 is overexpressed and induces fibroblast chemotaxis in the skin of sclerodermatous GVHD in a murine model (2010)
  • Author(s): Yamamoto A, Ashihara E, Nakagawa Y, Obayashi H, Ohta M, Hara H, Adachi T, Seno T, Kadoya M, Hamaguchi M, Ishino H, Kohno M, Maekawa T, Kawahito Y
  • Organizer: The 14th International Congress of Immunology
  • Place of Presentation: Kobe, Japan
• [Presentation] 関節リウマチにおけるAllograft inflammatory factor-1のサイトカイン・ケモカイン産生能および細胞遊走能についての検討
  • Author(s): 山本相浩
  • Organizer: 第40回日本臨床免疫学会
  • Place of Presentation: 東京