Molecular basis of febrile seizure and new treatment

• Project/Area Number: 22591130
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Pediatrics
• Research Institution: Okayama University
• Principal Investigator: INOUE Takushi 岡山大学, 岡山大学病院, 医員 (80572990)
• Co-Investigator(Kenkyū-buntansha): OHMORI Iori 岡山大学, 大学院・医歯薬学総合研究科, 助教 (20403488)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 熱性けいれん / 電位依存性ナトリウムチャネル / SCN1A遺伝子 / 二酸化炭素吸入療法 / 呼吸性アルカローシス / 発熱感受性

Research Abstract

The aim of this study was to assess the anticonvulsant effect of carbon dioxide (CO_2) on Scn1a mutation-related febrile seizures. We examined physiological changes in the blood gas levels after the induction of hyperthermia-induced seizures (HISs), which were associated with the Scn1a missense mutation. The Scn1a mutant rats demonstrated a higher HISs susceptibility associated with respiratory alkalosis than the WT rats. Inhalation of 10% CO_2 shortened the seizure duration. Blood gas analysis after the inhalation of 10% CO_2 demonstrated an elevated pCO_2 level and respiratory acidosis. Inhalation of 10% CO_2 demonstrated a potent and fast-acting anticonvulsant effect against HISs.

Research Products

• [Journal Article] Inhalation of 10% carbon dioxide rapidly terminates Scn1a mutation-related hyperthermia-induced seizures (2013)
  • Author(s): Ohmori I, Hayashi K, Wang H, Ouchida M, Fujita N, Inoue T, Michiue H, Nishiki T, Matsui H
  • Journal Title: Epilepsy Res Volume: in press Issue: 1-2 Pages: 999-999
  • DOI: 10.1016/j.eplepsyres.2013.01.003
  • NAID: 120006765093
  • Peer Reviewed
• [Journal Article] High-frequency EEG oscillations in hyperthermia-induced seizures of Scn1a mutant rats (2012)
  • Author(s): Kobayashi K, Ohmori I, Hayashi K, Kitagawa Y, Ouchida M, Inoue T, Ohtsuka Y
  • Journal Title: Epilepsy Res
  • Peer Reviewed
• [Journal Article] High-frequency EEG oscillations in hyperthermia-induced seizures of Scn1a mutant rats. (2012)
  • Author(s): Kobayashi K
  • Journal Title: Epilepsy Research Volume: 103 Issue: 2-3 Pages: 161-166
  • DOI: 10.1016/j.eplepsyres.2012.07.020
  • Peer Reviewed
• [Journal Article] Amplitude-integrated EEG colored according to spectral edge frequency (2011)
  • Author(s): Kobayashi K
  • Journal Title: Epilepsy Research Volume: 96 Issue: 3 Pages: 276-82
  • DOI: 10.1016/j.eplepsyres.2011.06.012
  • Peer Reviewed
• [Journal Article] High-frequency oscillations in idiopathic partial epilepsy of childhood (2011)
  • Author(s): Kobayashi K
  • Journal Title: Epilepsia Volume: 52 Issue: 10 Pages: 1812-1819
  • DOI: 10.1111/j.1528-1167.2011.03169.x
  • Peer Reviewed
• [Journal Article] Therapy of hyperthermia-induced seizures in Scn1a mutant rats. (2011)
  • Author(s): Hayashi Keiichiro
  • Journal Title: Epilepsia Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] Genetic seizure susceptibility underlying acute encephalopathies in childhood. (2010)
  • Author(s): Kobayashi Katsuhiro
  • Journal Title: Epilepsy Research Volume: 91 Pages: 143-152
  • Peer Reviewed
• [Presentation] 乳児期の外的脳障害により West 症候群を発症した3例 (2013)
  • Author(s): 井上拓志、岡牧郎、吉永治美、大塚頌子
  • Organizer: 第43回中国・四国点頭てんかん研究会
  • Place of Presentation: 岡山
  • Year and Date: 2013-03-23
• [Presentation] 乳児期の外的脳障害により West 症候群を発症した３例 (2013)
  • Author(s): 井上拓志
  • Organizer: 第 43 回中国・四国点頭てんかん研究会
  • Place of Presentation: 岡山
• [Presentation] ミトコンドリアDNA 13513G>A変異を有するLeigh脳症の1例 (2012)
  • Author(s): 井上拓志、竹内章人、岡牧郎、吉永治美、大塚頌子
  • Organizer: 第115回日本小児科学会学術集会
  • Place of Presentation: 福岡
  • Year and Date: 2012-04-21
• [Presentation] ミトコンドリアDNA 13513G＞A変異を有するLeigh脳症の１例 (2012)
  • Author(s): 井上拓志
  • Organizer: 第 115 回日本小児科学会学術集会
  • Place of Presentation: 福岡
• [Presentation] 内側側頭葉てんかんの頭皮上および頭蓋内脳波所見と臨床症状 (2011)
  • Author(s): 井上拓志
  • Organizer: 第45回日本てんかん学会
  • Place of Presentation: 新潟コンベンションセンター
  • Year and Date: 2011-10-06
• [Presentation] Scn1a遺伝子変異による熱性けいれんの二酸化炭素吸入療法 (2011)
  • Author(s): 大守伊織、大内田守、Wang Haijiao、林桂一郎、松井秀樹
  • Organizer: 第45回日本てんかん学会
  • Place of Presentation: 新潟
• [Presentation] ワークショップ てんかんモデル動物の研究から臨床応用へ「全般てんかん熱性痙攣プラスモデルラットの開発と臨床研究への応用」 (2010)
  • Author(s): 大守伊織
  • Organizer: 第44回日本てんかん学会
  • Place of Presentation: 岡山市
• [Remarks] ホームページ
  • URL: http://www.okayama-u.ac.jp/user/cneuro/