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Gene expression profile induced by viral infection during asthma exacerbation

Research Project

Project/Area Number 22591151
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Pediatrics
Research InstitutionTsukuba University of Technology

Principal Investigator

SHIBASAKI Masanao  筑波技術大学, 保険科学部, 教授 (30049233)

Co-Investigator(Kenkyū-buntansha) ARINAMI Tadao  筑波大学, 医学医療系, 教授 (10212648)
NOGUCHI Emiko  筑波大学, 医学医療系, 准教授 (40344882)
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2012: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords喘息 / ウイルス感染 / マイクロアレイ / ウイルス
Research Abstract

It is well known that asthma exacerbation in pediatric patients is often induced by respiratory viral infection. In the present study, we examined gene expression profile induced by viral infection during asthma exacerbation. Pediatric patients (n=16) who admitted to the hospital due to asthma exacerbation were enrolled in the study. Blood was taken twice from each patient, once during asthma exacerbation and once during a stable period. Total RNA was extracted from peripheral blood mononuclear cells and subjected to microarray analysis. Sixty-five/5 genes were significantly up/down-regulated during asthma exacerbation by microarray analysis. Gene ontology analysis revealed that 21 genes were related to immune reaction, 16 genes for immune reaction against pathogens, 21 genes for defense responses against pathogens, and 7 genes for interferon reactions.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (21 results)

All 2013 2012 2011 2010 Other

All Journal Article (13 results) (of which Peer Reviewed: 11 results) Presentation (8 results) (of which Invited: 1 results)

  • [Journal Article] A Distinct Sensitization Pattern Associated with Asthma and the Thymic Stromal Lymphopoietin (<i>TSLP</i>) Genotype2013

    • Author(s)
      Iijima H
    • Journal Title

      Allergology International

      Volume: 62 Issue: 1 Pages: 123-130

    • DOI

      10.2332/allergolint.12-OA-0488

    • NAID

      130004477178

    • ISSN
      1323-8930, 1440-1592
    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Asthma Phenotypes in Japanese Adults - Their Associations with the <i>CCL5</i> and <i>ADRB2</i> Genotypes2013

    • Author(s)
      Kaneko Y
    • Journal Title

      Allergology International

      Volume: 62 Issue: 1 Pages: 113-121

    • DOI

      10.2332/allergolint.12-OA-0467

    • NAID

      130004477174

    • ISSN
      1323-8930, 1440-1592
    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Asthma Phenotypes in Japanese Adults-Their Associations with the CCL5 and ADRB2 Genotypes2012

    • Author(s)
      Kaneko Y, Masuko H, Sakamoto T, Iijima H, Naito T, Yatagai Y, Yamada H, Konno S, Nishimura M, Noguchi E, Hizawa N
    • Journal Title

      Allergol Int

    • NAID

      130004477174

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] A Distinct Sensitization Pattern Associated with Asthma and the Thymic Stromal Lymphopoietin (TSLP) Genotype2012

    • Author(s)
      Iijima H, Kaneko Y, Yamada H, Yatagai Y, Masuko H, Sakamoto T, Naito T, Hirota T, Tamari M, Konno S, Nishimura M, Noguchi E, Hizawa N
    • Journal Title

      Allergol Int

    • NAID

      130004477178

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Genome-Wide Association Study Identifies HLA-DP as a Susceptibility Gene for Pediatric Asthma in Asian Populations2011

    • Author(s)
      Noguchi E, Sakamoto H, Hirota T, Ochiai K, Imoto Y, Sakashita M, Kurosaka F, Akasawa A, Yoshihara S, Kanno N, Yamada Y, Shimojo N, Kohno Y, Suzuki Y, Kang MJ, Kwon JW, Hong SJ, Inoue K, Goto Y, Yamashita F, Asada T, Hirose H, Saito I, Fujieda S, Hizawa N, Sakamoto T, Masuko H, Nakamura Y, Nomura I, Tamari M, Arinami T, Yoshida T, Saito H, Matsumoto K
    • Journal Title

      PLos Genet

      Volume: 7:e1002170

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Upregulation of IL17RB during Natural Allergen Exposure in Patients with Seasonal Allergic Rhinitis2011

    • Author(s)
      Matsumoto Y, Noguchi E, Imoto Y, Nanatsue K, Takeshita K, Shibasaki M, Arinami T, Fujieda S
    • Journal Title

      Allergol Int

      Volume: 60 Pages: 87-92

    • NAID

      10031143303

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Variants of C-C motif chemokine 22 (CCL22) are associated with susceptibility to atopic dermatitis: case-control studies2011

    • Author(s)
      Hirota T, Saeki H, Tomita K, Tanaka S, Ebe K, Sakashita M, Yamada T, Fujieda S, Miyatake A, Doi S, Enomoto T, Hizawa N, Sakamoto T, Masuko H, Sasaki T, Ebihara T, Amagai M, Esaki H, Takeuchi S, Furue M, Noguchi E, Kamatani N, Nakamura Y, Kubo M, Tamari M
    • Journal Title

      PLoS One

      Volume: 6:e26987

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] アレルギー疾患のトランスクリプトーム解析とプロテオミクス2011

    • Author(s)
      野口恵美子
    • Journal Title

      小児科

      Volume: 52 Pages: 823-832

    • Related Report
      2012 Final Research Report
  • [Journal Article] アレルギー疾患発症とIgE産生能にかかわる遺伝要因2011

    • Author(s)
      野口恵美子
    • Journal Title

      アレルギーの臨床

      Volume: 415 Pages: 392-396

    • Related Report
      2012 Final Research Report
  • [Journal Article] Upregulation of IL17RB during natural allergen exposure in patients with seasonal allergic rhinitis2011

    • Author(s)
      Matsumoto Y.
    • Journal Title

      Allergol Int i

      Volume: 60 Pages: 87-92

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Apolipoprotein A-IV is a candidate target molecule for the treatment of seasonal allergic rhinitis2010

    • Author(s)
      Makino Y, Noguchi E, Takahashi N, Matsumoto Y, Kubo S, Yamada T, Imoto Y, Ito Y, Osawa Y, Shibasaki M, Uchida K, Meno K, Suzuki H, Okubo K, Arinami T, Fujieda S
    • Journal Title

      J Allergy Clin Immunol

      Volume: 126 Pages: 1163-9

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] S2554X mutation in the filaggrin gene is associated with allergen sensitization in the Japanese population2010

    • Author(s)
      Imoto Y, Enomoto H, Fujieda S, Okamoto M, Sakashita M, Susuki D, Okada M, Hirota T, Tamari M, Ebe K, Arinami T, Noguchi E
    • Journal Title

      J Allergy Clin Immunol

      Volume: 125 Pages: 498-500

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Apolipoprotein A-IV is a candidate target molecule for the treatment of seasonal allergic_rhinitis2010

    • Author(s)
      Makino Y.
    • Journal Title

      J Allergy Clin Immunol

      Volume: 126 Pages: 1163-1169

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] 次世代シーケンサーを用いたWhole Transcriptome解析によるスギ花粉症関連遺伝子の同定2012

    • Author(s)
      徳永貴広, 意元義政, 野口恵美子, 有波忠雄, 藤枝重治
    • Organizer
      第62回日本アレルギー学会秋季学術大会
    • Place of Presentation
      大阪
    • Year and Date
      2012-11-30
    • Related Report
      2012 Final Research Report
  • [Presentation] 次世代シークエンサーのアレルギー疾患研究への応用2012

    • Author(s)
      野口恵美子
    • Organizer
      第24回日本アレルギー学会春季臨床大会
    • Place of Presentation
      大阪
    • Year and Date
      2012-05-12
    • Related Report
      2012 Final Research Report
  • [Presentation] 鼻におけるアレルギー性鼻炎関連遺伝子との関連2011

    • Author(s)
      意元義政, 野口恵美子, 藤枝重治
    • Organizer
      第61回日本アレルギー学会秋季学術大会
    • Place of Presentation
      東京
    • Year and Date
      2011-11-10
    • Related Report
      2012 Final Research Report
  • [Presentation] 遺伝子の情報をどのように臨床にむすびつけていくか?ゲノム解析とトランスクリプトーム解析2011

    • Author(s)
      野口恵美子
    • Organizer
      第11回愛知免疫アレルギーを語る会
    • Place of Presentation
      名古屋
    • Year and Date
      2011-04-23
    • Related Report
      2012 Final Research Report
  • [Presentation] ゲノム解析によるアレルギー疾患の疾患感受性遺伝子の同定2011

    • Author(s)
      野口恵美子
    • Organizer
      関東アレルギークラブ
    • Place of Presentation
      東京
    • Year and Date
      2011-02-12
    • Related Report
      2012 Final Research Report
  • [Presentation] 副鼻腔炎重治 網羅的遺伝子解析によるスギ花粉症発症に関する遺伝子の同定2010

    • Author(s)
      意元義政, 野口恵美子, 有波忠雄, 藤枝 重治
    • Organizer
      第60回日本アレルギー学会秋季学術大会
    • Place of Presentation
      東京
    • Year and Date
      2010-11-26
    • Related Report
      2012 Final Research Report
  • [Presentation] 次世代シーケンサーを用いたWhole Transcriptome解析によるスギ花粉症関連遺伝子の同定

    • Author(s)
      徳永貴広
    • Organizer
      第62回日本アレルギー学会秋季学術大会
    • Place of Presentation
      大阪国際会議場(大阪府)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 次世代シークエンサーのアレルギー疾患研究への応用

    • Author(s)
      野口恵美子
    • Organizer
      第24回日本アレルギー学会春季臨床大会
    • Place of Presentation
      大阪国際会議場(大阪府)
    • Related Report
      2012 Annual Research Report
    • Invited

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Published: 2010-08-23   Modified: 2019-07-29  

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