Molecular mechanisms of peripheral blood stem cell mobilization through Interleukin-8 and its clinical application

• Project/Area Number: 22591164
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Pediatrics
• Research Institution: Kagoshima University
• Principal Investigator: OKAMOTO Yasuhiro 鹿児島大学, 医学部・歯学部附属病院, 講師 (30398002)
• Co-Investigator(Kenkyū-buntansha): KAWANO Yoshifumi 鹿児島大学, 医歯学総合研究科, 教授 (20260680)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: G-CSF / 血管内皮細胞 / IL-8 / TLR / endothelial cell / mobilization / 動員 / 好中球 / インターロイキン-8 / 幹細胞 / 血管内皮

Research Abstract

Several genes were elucidated by the expression pattern when HUVEC was co-cultured with G-CSF stimulated neutrophils. Moreover, mRNA was confirmed by real time PCR and surface expression was confirmed by FACS. Within several genes, antibody to TLR2 or inhibitory peptide of TLR2 signaling decreased the IL-8 production in HUVEC as low as 35 to 50 %.

Research Products

• [Journal Article] Vascular endothelial growth factor corrected for platelet count and hematocrit is associated with the clinical course of aplastic anemia in children (2012)
  • Author(s): Kodama Y, et al
  • Journal Title: Int J Hematol Volume: (In press) Issue: 5 Pages: 494-499
  • DOI: 10.1007/s12185-012-1074-1
  • Peer Reviewed
• [Journal Article] Benzene Metabolite, 1,2,4-Benzenetriol, Induces Halogenated DNA and Tyrosines Representing Halogenative Stress in Human Myeloid Cell Line HL-60 (2012)
  • Author(s): Nishikawa T, Miyahara E, Horiuchi M, Izumo K, Okamoto Y, Kawai Y, Kawano Y, Takeuchi T
  • Journal Title: Environ Health Perspect Volume: 120 Issue: 1 Pages: 62-67
  • DOI: 10.1289/ehp.1103437
  • Peer Reviewed
• [Journal Article] Benzene induces cytotoxicity without metabolic activation (2011)
  • Author(s): Nishikawa T, Izumo K, Miyahara E, Horiuchi M, Okamoto Y, Kawano Y, Takeuchi T
  • Journal Title: J Occup Health Volume: 53 Pages: 84-92
  • NAID: 10027959345
  • Peer Reviewed
• [Journal Article] Acute respiratory distress syndrome as an initial presentation of hemophagocytic lymphohistiocytosis after induction therapy for acute myeloid leukemia (2011)
  • Author(s): Nishikawa T, Okamoto Y, Tanabe T, Shinkoda Y, Kodama Y, Kakihana Y, Goto M, Kawano Y
  • Journal Title: Pediatr Hematol Oncol Volume: 3 Issue: 3 Pages: 244-8
  • DOI: 10.3109/08880018.2010.514038
  • Peer Reviewed
• [Journal Article] Benzene Induces Cytotoxicity without Metabolic Activation (2011)
  • Author(s): Nishikawa T, Izumo K, Miyahara E, Horiuchi M, Okamoto Y, Kawano Y, Takeuchi T
  • Journal Title: Journal of Occupational Health Volume: 53 Issue: 2 Pages: 84-92
  • DOI: 10.1539/joh.10-002-OA
  • NAID: 130004447538
  • ISSN: 1341-9145, 1348-9585
  • Peer Reviewed
• [Journal Article] Serum derivative of reactive oxygen metabolites (d-ROMs) in pediatric hemato-oncological patients with neutropenic fever (2010)
  • Author(s): Nishikawa T, Okamoto Y, Kodama Y, Tanabe T, Shinkoda Y, Kawano Y
  • Journal Title: Pediatr Blood Cancer 55 Volume: 55 Issue: 1 Pages: 91-94
  • DOI: 10.1002/pbc.22507
  • Peer Reviewed
• [Journal Article] Successful Bone Marrow Transplantation for Children with Aplastic Anemia based on a Best-Available Evidence Strategy (2010)
  • Author(s): Okamoto Y, Kodama Y, Nishikawa T, Yamaki Y, Mougi H, Masamoto I, Tanabe T, Shinkoda Y, Kawano Y
  • Journal Title: Pediatr Transplant Volume: 14 Issue: 8 Pages: 980-985
  • DOI: 10.1111/j.1399-3046.2010.01388.x
  • Peer Reviewed
• [Journal Article] Unexpectedly High AUC Levels in a Child who Received Intravenous Busulfan prior to Stem Cell Transplantation. (2010)
  • Author(s): Nishikawa T, Okamoto Y, et al
  • Journal Title: Bone Marrow Transplant Volume: 45 Pages: 602-604
  • Peer Reviewed
• [Journal Article] Serum derivative of reactive oxygen metabolites (d-ROMs) in pediatric hemato-oncological patients with neutropenic fever. (2010)
  • Author(s): Nishikawa T, Okamoto Y, et al
  • Journal Title: Pediatr Blood Cancer Volume: 55 Pages: 91-94
  • Peer Reviewed
• [Journal Article] Successful Bone Marrow Transplantation for Children with Aplastic Anemia based on a Best-Available Evidence Strategy. (2010)
  • Author(s): Okamoto Y, Kodama Y, et al.
  • Journal Title: Pediatr Transplantation Volume: 14 Pages: 980-985
  • Peer Reviewed
• [Presentation] ナノ粒子を用いた高感度qPCR法による造血幹細胞移植後のウイルス感染／再活性化の早期診断およびモニタリング法の確立 (2013)
  • Author(s): 岡本康裕
  • Organizer: 第35回日本造血細胞移植学会
  • Place of Presentation: Kanazawa
• [Presentation] 小児血液腫瘍患者の DICに対するトロンボモジュリン製剤の安全性と効果に関する検討 (2012)
  • Author(s): 岡本康裕
  • Organizer: 第24回日本小児血液学会総会
  • Place of Presentation: 大阪
  • Year and Date: 2012-12-19
• [Presentation] Combined analysis of treatments for pediatric ALL in KYCCSG protocols (2012)
  • Author(s): Okamoto Y
  • Organizer: 第74回日本血液学会学術集会
  • Place of Presentation: 京都
  • Year and Date: 2012-10-21
• [Presentation] Transplant for aplastic anemia (2012)
  • Author(s): Okamoto Y
  • Organizer: St.Jude-VIVA Forum in Pediatric Oncology
  • Place of Presentation: Singapore
  • Year and Date: 2012-03-04
• [Presentation] 小児急性骨髄性白血病の非寛解期の造血細胞移植術の成績と予後因子の検討 (2012)
  • Author(s): 岡本康裕
  • Organizer: 第34回日本造血細胞移植学会
  • Place of Presentation: 大阪
  • Year and Date: 2012-02-25
• [Presentation] 小児急性リンパ性白血病の非寛解期の造血細胞移植術の成績と予後因子の検討 (2012)
  • Author(s): 岡本康裕
  • Organizer: 第34回日本造血細胞移植学会
  • Place of Presentation: 大阪
  • Year and Date: 2012-02-25
• [Presentation] ダウン症に合併した脳腫瘍の 2 例 (2011)
  • Author(s): 岡本康裕
  • Organizer: 第25回日本小児血液・がん学会
  • Place of Presentation: 前橋
  • Year and Date: 2011-11-27
• [Presentation] ダウン症に合併した脳腫瘍の2例 (2011)
  • Author(s): 岡本康裕、八牧愉二、児玉祐一、新小田雄一、田邊貴幸、西川拓朗、倉内宏一郎、義岡孝子、米澤大、平野宏文、有田和徳、河野嘉文
  • Organizer: 第25回日本小児血液・がん学会
  • Place of Presentation: 前橋市
  • Year and Date: 2011-11-25
• [Presentation] 治療の大幅な減量、治療中止後に寛解を維持しているALLの3例 (2011)
  • Author(s): 岡本康裕、児玉祐一、西川拓朗、八牧愉二、倉内宏一郎、田邊貴幸、新小田雄一、河野嘉文
  • Organizer: 第72回日本血液学会
  • Place of Presentation: 名古屋市
  • Year and Date: 2011-10-16
• [Presentation] 治療の大幅な減量、治療中止後に寛解を維持している ALL の 3 例 (2011)
  • Author(s): 岡本康裕
  • Organizer: 第72回日本血液学会
  • Place of Presentation: 名古屋
  • Year and Date: 2011-10-06
• [Presentation] 急性骨髄性白血病(AML)に対する順化NK細胞輸注(NK-DLI)の経験 (2010)
  • Author(s): 岡本康裕, 他
  • Organizer: 日本小児血液学会総会
  • Place of Presentation: 大阪
  • Year and Date: 2010-12-18
• [Presentation] 小児血液腫瘍患者のDICに対するトロンボモジュリン製剤の安全性と効果に関する検討 (2010)
  • Author(s): 岡本康裕, 他
  • Organizer: 日本小児血液学会総会
  • Place of Presentation: 大阪
  • Year and Date: 2010-12-18
• [Presentation] Combined analysis of treatments for pediatric T-ALL in KYCCSG protocols (2010)
  • Author(s): Okamoto Y
  • Organizer: 第72回日本血液学会学術集会
  • Place of Presentation: 横浜
  • Year and Date: 2010-09-26
• [Presentation] Pharmacokinetic study of intravenous busulfan in hematopoietic stem cell transplantation (2010)
  • Author(s): Nagatoshi Y, Okamoto Y
  • Organizer: results of a prospective study with 25 children. 36thAnnual Meeting of the EBMT
  • Place of Presentation: Vienna, Austria
  • Year and Date: 2010-03-24
• [Presentation] Treatment outcome of KYCCSG ALL96 protocol (2010)
  • Author(s): Okamoto Y
  • Organizer: St. Jude - VIVA Forum in Pediatric Oncology 2010
  • Place of Presentation: Singapore
  • Year and Date: 2010-03-05