| Project/Area Number |
22591412
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | Fukushima Medical University |
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Principal Investigator |
ISE Kazuya 福島県立医科大学, 医学部, 講師 (90363746)
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| Co-Investigator(Kenkyū-buntansha) |
YAMASHITA Michitoshi 福島県立医科大学, 医学部, 助教 (20381387)
SAITO Takahiro 福島県立医科大学, 医学部, 博士研究員 (00566812)
SHIMIZU Hirofumi 福島県立医科大学, 医学部, 助教 (70553709)
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| Co-Investigator(Renkei-kenkyūsha) |
GOTOH Mitsukazu 福島県立医科大学, 医学部, 教授 (50162160)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2010: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
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| Keywords | I 型糖尿病 / 膵島移植 / 膵島再生 / 細胞シート / ティッシュエンジニアリング / I型糖尿病 |
|---|
| Research Abstract |
Pancreatic islets were obtained from Lewis rats, and dissociated islet cells were obtained by incubating with trypsin-EDTA. Dissociated islet cells were then plated onto the PIPAAm dishes. After reaching confluency at day 2, islet cells were successfully harvested as a uniformly spread islet sheet by lowering the culture temperature. We then conducted islet sheet transplantation procedure to create neo-islet tissues into subcutaneous space of Streptozotocin-induced diabetic SCID mice. Histological analyses revealed that strip-shaped neo-islet tissues in the subcutaneous space. This neo-islet engineering approach successfully converted the hyperglycemia to euglycemia in all the recipients and sustained the therapeutic effects for 300 days (length of the experiments). We then conducted intra-peritoneal glucose tolerant tests to the recipients, and found that the recipient SCID mice showed glucose changes that are identical to normal mice
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