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Development of molecular diagnostic of minimal residual disease ofgastric cancer in peritoneal fluids

Research Project

Project/Area Number 22591468
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Digestive surgery
Research InstitutionKitasato University

Principal Investigator

KIKUCHI Shiro  北里大学, 医学部, 准教授 (30161417)

Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords胃癌 / 腹水 / DNA診断 / CDO1 / メチル化 / 微小癌細胞 / HOPX / Reprimo / 腹水洗浄細胞診
Research Abstract

We have identified cancer-specific methylation genes as HOPX, Reprimo, and CDO1. HOPX was not detected in peritoneal fluids of advanced gastric cancer, so we started to examine CDO1 potential for minimal residual disease. CDO1 could be detected for cancer cells with CDO1 methylation by 1/1000 dilution. Plasma of cancer patients showed poor quality of DNA, because sensitivity of beta-actin exhibitedso. DNA detection in plasma of cancer patients showed in 30% of advanced gastric cancer, shiwh is similar with classical tumor marker. So, we think that novel device is requiredfor clinical application of DNA methylation by nested PCR or acquisition of good quality DNA.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (13 results)

All 2013 2012 2011 2010

All Journal Article (9 results) (of which Peer Reviewed: 9 results) Presentation (4 results)

  • [Journal Article] Laparoscopy versus open distal gastrectomy by expert surgeons for early gastric cancer in Japanese patients: short-term clinical outcomes of a randomized clinical trial2013

    • Author(s)
      Sakuramoto S, Yamashita K, Kikuchi S, Futawatari N, Katada N, Watanabe M, Okutomi T, Wang G, Bax L
    • Journal Title

      Surg Endosc

      Volume: 27(5) Pages: 1695-705

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Survival outcome of laparoscopic gastrectomy for clinical early (cT1) gastric cancer2012

    • Author(s)
      Yamashita K, Sakuramoto S, Shibata T, Nemoto M, Mieno H, Katada N, Kikuchi S, Watanabe M
    • Journal Title

      Surg Today

    • NAID

      10031194467

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Recent trends in the management of achalasia2012

    • Author(s)
      Katada N, Sakuramoto S, Yamashita K, Shibata T, Moriya H, Kikuchi S, Watanabe M
    • Journal Title

      Ann Thorac Cardiovasc Surg

      Volume: 18(5) Pages: 420-8

    • NAID

      10031120924

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Trend in gastric cancer: 35 years of surgical experience in Japan2011

    • Author(s)
      Yamashita K, Sakuramoto S, Nemoto M, Shibata T, Mieno H, Katada N, Kikuchi S, Watanabe M
    • Journal Title

      World J Gastroenterol

      Volume: 17(29) Issue: 29 Pages: 3390-7

    • DOI

      10.3748/wjg.v17.i29.3390

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Comparison of staging between the old (6th edition) and new (7th edition) TNM classifications in advanced gastric cancer2011

    • Author(s)
      Kikuchi S, Futawatari N, Sakuramoto S, Katada N, Yamashita K, Shibata T,Nemoto M, Watanabe M
    • Journal Title

      Anticancer Res

      Volume: 31(6) Pages: 2361-5

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Therapeutic potential of PRL-3 targeting and clinical significance of PRL-3 genomic amplification in gastric cancer2011

    • Author(s)
      Ooki A, Yamashita K, Kikuchi S, Sakuramoto S, Katada N, Waraya M, Kawamata H, Nishimiya H, Nakamura K, Watanabe M
    • Journal Title

      BMC Cancer

      Volume: 11 Issue: 1 Pages: 122-122

    • DOI

      10.1186/1471-2407-11-122

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Potential utility of HOP homeobox gene promoter methylation as a marker of tumor aggressiveness in gastric cancer2010

    • Author(s)
      Ooki A, Yamashita K, Kikuchi S, Sakuramoto S, Katada N, Kokubo K, Kobayashi H, Kim MS, Sidransky D, Watanabe M
    • Journal Title

      Oncogene

      Volume: 29(22) Issue: 22 Pages: 3263-75

    • DOI

      10.1038/onc.2010.76

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Phosphatase of regenerating liver-3 as a convergent therapeutic target for lymph node metastasis in esophageal squamous cell carcinoma2010

    • Author(s)
      Ooki A, Yamashita K, Kikuchi S, Sakuramoto S, Katada N, Watanabe M
    • Journal Title

      Int J Cancer

      Volume: 127(3) Issue: 3 Pages: 543-54

    • DOI

      10.1002/ijc.25082

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Technique of esophagojejunostomy using transoral placement of the pretilted anvil head after laparoscopic gastrectomy for gastric cancer2010

    • Author(s)
      Sakuramoto S, Kikuchi S, Futawatari N, Moriya H, Katada N, Yamashita K,Watanabe M
    • Journal Title

      Surgery

      Volume: 147(5) Issue: 5 Pages: 742-7

    • DOI

      10.1016/j.surg.2009.06.016

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Presentation] 胃癌に対する腹腔鏡下胃全摘術:安全性と有効性について (ワークショップ)2012

    • Author(s)
      山下継史、桜本信一、片田夏也、柴田智隆、三重野浩朗、根本昌之、菊池史郎、渡邊昌彦
    • Organizer
      第84回日本胃癌学会
    • Place of Presentation
      大阪
    • Year and Date
      2012-02-10
    • Related Report
      2012 Final Research Report
  • [Presentation] 進行胃癌術後化学療法の個別化を目指したリンパ節転移密度 (ND値)の予後的意義 (シンポジウム)2012

    • Author(s)
      江間玲、山下継史、桜本信一、柴田智隆、三重野浩朗、根本昌之、片田夏也、菊池史郎、渡邊昌彦
    • Organizer
      第84回日本胃癌学会
    • Place of Presentation
      大阪
    • Year and Date
      2012-02-10
    • Related Report
      2012 Final Research Report
  • [Presentation] 腹腔鏡下胃切除術前の 32-MDCT Angiography を用いた肝動脈走行の評価 (要望演題)2011

    • Author(s)
      山下継史、桜本信一、三重野浩朗、柴田智隆、根本昌之、片田夏也、菊池史郎、渡邊昌彦
    • Organizer
      第24回日本内視鏡外科学会
    • Place of Presentation
      大阪
    • Year and Date
      2011-12-07
    • Related Report
      2012 Final Research Report
  • [Presentation] Preoperative 3D CT Angiography Assessment of Right Hepatic Artery Before Laparoscopic Gastrectomy2011

    • Author(s)
      Yamashita K., Sakuramoto S., Mieno H., Nemoto M., Shibata T., Katada N., Kikuchi S., and Masahiko Watanabe M
    • Organizer
      IASGO (Symposium)
    • Place of Presentation
      Tokyo, Japan
    • Year and Date
      2011-11-10
    • Related Report
      2012 Final Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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