Development of molecular diagnostic of minimal residual disease ofgastric cancer in peritoneal fluids

• Project/Area Number: 22591468
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Kitasato University
• Principal Investigator: KIKUCHI Shiro 北里大学, 医学部, 准教授 (30161417)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 胃癌 / 腹水 / DNA診断 / CDO1 / メチル化 / 微小癌細胞 / HOPX / Reprimo / 腹水洗浄細胞診

Research Abstract

We have identified cancer-specific methylation genes as HOPX, Reprimo, and CDO1. HOPX was not detected in peritoneal fluids of advanced gastric cancer, so we started to examine CDO1 potential for minimal residual disease. CDO1 could be detected for cancer cells with CDO1 methylation by 1/1000 dilution. Plasma of cancer patients showed poor quality of DNA, because sensitivity of beta-actin exhibitedso. DNA detection in plasma of cancer patients showed in 30% of advanced gastric cancer, shiwh is similar with classical tumor marker. So, we think that novel device is requiredfor clinical application of DNA methylation by nested PCR or acquisition of good quality DNA.

Research Products

• [Journal Article] Laparoscopy versus open distal gastrectomy by expert surgeons for early gastric cancer in Japanese patients: short-term clinical outcomes of a randomized clinical trial (2013)
  • Author(s): Sakuramoto S, Yamashita K, Kikuchi S, Futawatari N, Katada N, Watanabe M, Okutomi T, Wang G, Bax L
  • Journal Title: Surg Endosc Volume: 27(5) Pages: 1695-705
  • Peer Reviewed
• [Journal Article] Survival outcome of laparoscopic gastrectomy for clinical early (cT1) gastric cancer (2012)
  • Author(s): Yamashita K, Sakuramoto S, Shibata T, Nemoto M, Mieno H, Katada N, Kikuchi S, Watanabe M
  • Journal Title: Surg Today
  • NAID: 10031194467
  • Peer Reviewed
• [Journal Article] Recent trends in the management of achalasia (2012)
  • Author(s): Katada N, Sakuramoto S, Yamashita K, Shibata T, Moriya H, Kikuchi S, Watanabe M
  • Journal Title: Ann Thorac Cardiovasc Surg Volume: 18(5) Pages: 420-8
  • NAID: 10031120924
  • Peer Reviewed
• [Journal Article] Trend in gastric cancer: 35 years of surgical experience in Japan (2011)
  • Author(s): Yamashita K, Sakuramoto S, Nemoto M, Shibata T, Mieno H, Katada N, Kikuchi S, Watanabe M
  • Journal Title: World J Gastroenterol Volume: 17(29) Issue: 29 Pages: 3390-7
  • DOI: 10.3748/wjg.v17.i29.3390
  • Peer Reviewed
• [Journal Article] Comparison of staging between the old (6th edition) and new (7th edition) TNM classifications in advanced gastric cancer (2011)
  • Author(s): Kikuchi S, Futawatari N, Sakuramoto S, Katada N, Yamashita K, Shibata T,Nemoto M, Watanabe M
  • Journal Title: Anticancer Res Volume: 31(6) Pages: 2361-5
  • Peer Reviewed
• [Journal Article] Therapeutic potential of PRL-3 targeting and clinical significance of PRL-3 genomic amplification in gastric cancer (2011)
  • Author(s): Ooki A, Yamashita K, Kikuchi S, Sakuramoto S, Katada N, Waraya M, Kawamata H, Nishimiya H, Nakamura K, Watanabe M
  • Journal Title: BMC Cancer Volume: 11 Issue: 1 Pages: 122-122
  • DOI: 10.1186/1471-2407-11-122
  • Peer Reviewed
• [Journal Article] Potential utility of HOP homeobox gene promoter methylation as a marker of tumor aggressiveness in gastric cancer (2010)
  • Author(s): Ooki A, Yamashita K, Kikuchi S, Sakuramoto S, Katada N, Kokubo K, Kobayashi H, Kim MS, Sidransky D, Watanabe M
  • Journal Title: Oncogene Volume: 29(22) Issue: 22 Pages: 3263-75
  • DOI: 10.1038/onc.2010.76
  • Peer Reviewed
• [Journal Article] Phosphatase of regenerating liver-3 as a convergent therapeutic target for lymph node metastasis in esophageal squamous cell carcinoma (2010)
  • Author(s): Ooki A, Yamashita K, Kikuchi S, Sakuramoto S, Katada N, Watanabe M
  • Journal Title: Int J Cancer Volume: 127(3) Issue: 3 Pages: 543-54
  • DOI: 10.1002/ijc.25082
  • Peer Reviewed
• [Journal Article] Technique of esophagojejunostomy using transoral placement of the pretilted anvil head after laparoscopic gastrectomy for gastric cancer (2010)
  • Author(s): Sakuramoto S, Kikuchi S, Futawatari N, Moriya H, Katada N, Yamashita K,Watanabe M
  • Journal Title: Surgery Volume: 147(5) Issue: 5 Pages: 742-7
  • DOI: 10.1016/j.surg.2009.06.016
  • Peer Reviewed
• [Presentation] 胃癌に対する腹腔鏡下胃全摘術:安全性と有効性について (ワークショップ) (2012)
  • Author(s): 山下継史、桜本信一、片田夏也、柴田智隆、三重野浩朗、根本昌之、菊池史郎、渡邊昌彦
  • Organizer: 第84回日本胃癌学会
  • Place of Presentation: 大阪
  • Year and Date: 2012-02-10
• [Presentation] 進行胃癌術後化学療法の個別化を目指したリンパ節転移密度 (ND値)の予後的意義 (シンポジウム) (2012)
  • Author(s): 江間玲、山下継史、桜本信一、柴田智隆、三重野浩朗、根本昌之、片田夏也、菊池史郎、渡邊昌彦
  • Organizer: 第84回日本胃癌学会
  • Place of Presentation: 大阪
  • Year and Date: 2012-02-10
• [Presentation] 腹腔鏡下胃切除術前の 32-MDCT Angiography を用いた肝動脈走行の評価 (要望演題) (2011)
  • Author(s): 山下継史、桜本信一、三重野浩朗、柴田智隆、根本昌之、片田夏也、菊池史郎、渡邊昌彦
  • Organizer: 第24回日本内視鏡外科学会
  • Place of Presentation: 大阪
  • Year and Date: 2011-12-07
• [Presentation] Preoperative 3D CT Angiography Assessment of Right Hepatic Artery Before Laparoscopic Gastrectomy (2011)
  • Author(s): Yamashita K., Sakuramoto S., Mieno H., Nemoto M., Shibata T., Katada N., Kikuchi S., and Masahiko Watanabe M
  • Organizer: IASGO (Symposium)
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 2011-11-10