Analysis of tumor hypoxia in lung cancer using real time mass spectrometry

• Project/Area Number: 22591567
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Thoracic surgery
• Research Institution: Hiroshima University
• Principal Investigator: MIYATA Yoshihiro 広島大学, 原爆放射線医科学研究所, 准教授 (50397965)
• Co-Investigator(Kenkyū-buntansha): OKADA Morihito 広島大学, 原爆放射線医科学研究所, 教授 (70446045)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 呼吸器外科学 / FDG-PET / 肺癌 / メタボローム / 肺がん / 質量分析

Research Abstract

In lung adenocarcinoma, the SUVmax and the expression of HIF1α and GLUT1 correlated with various clinicopathological factors relating to malignancy and disease free survival. We conclude that in lung adenocarcinoma, but not squamous cell carcinoma, HIF1α-induced GLUT1 expression indicates tumor aggressiveness pathologically, and might explain high FDG uptake on PET and correlate with poor prognosis. We also show that proline and glutamate metabolism may play a role in 5-FU induced cell death using real time mass spectrometry.

Research Products

• [Journal Article] Metabolomic analysis of dynamic response and drug resistance of gastric cancer cells to 5-fluorouracil (2013)
  • Author(s): Sasada S, Miyata Y, Tsutani Y, Tsuyama N, Masujima T, Hihara J, Okada M
  • Journal Title: Oncol Rep Volume: 29(3) Pages: 925-31
  • Peer Reviewed
• [Journal Article] Prognostic significance of metabolic response by positron emission tomography after neoadjuvant chemotherapy for resectable malignant pleural mesothelioma (2013)
  • Author(s): Tsutani Y, Takuwa T, Miyata Y, Fukuoka K, Hasegawa S, Nakano T, Okada M
  • Journal Title: Ann Oncol Volume: 24(4) Pages: 1005-10
  • Peer Reviewed
• [Journal Article] Metabolomic analysis of dynamic response and drug resistance of gastric cancer cells to 5-fluorouracil (2013)
  • Author(s): Sasada S
  • Journal Title: Oncol Rep Volume: 29 Issue: 3 Pages: 925-931
  • DOI: 10.3892/or.2012.2182
  • Peer Reviewed
• [Journal Article] Solid tumors versus mixed tumors with a ground-glass opacity component in patients with clinical stage IA lung adenocarcinoma: Prognostic comparison using high-resolution computed tomography findings (2013)
  • Author(s): Tsutani Y
  • Journal Title: J Thorac Cardiovasc Surg Volume: Epub ahead of print Issue: 1 Pages: 1-7
  • DOI: 10.1016/j.jtcvs.2012.11.019
  • Peer Reviewed
• [Journal Article] Prognostic significance of metabolic response by positron emission tomography after neoadjuvant chemotherapy for resectable malignant pleural mesothelioma (2013)
  • Author(s): Tsutani Y
  • Journal Title: Ann Oncol Volume: 24 Issue: 4 Pages: 1005-1010
  • DOI: 10.1093/annonc/mds537
  • Peer Reviewed
• [Journal Article] Solid tumors versus mixed tumors with a ground-glass opacity component in patients with clinical stage IA lung adenocarcinoma: Prognostic comparison using high-resolution computed tomography findings (2012)
  • Author(s): Tsutani Y, Miyata Y, Yamanaka T, Nakayama H, Okumura S, Adachi S, Yoshimura M, Okada M
  • Journal Title: J Thorac Cardiovasc Surg
  • Peer Reviewed
• [Journal Article] Prediction of pathologic node-negative clinical stage IA lung adenocarcinoma for optimal candidates undergoing sublobar resection (2012)
  • Author(s): Tsutani Y, Miyata Y, Nakayama H, Okumura S, Adachi S, Yoshimura M, Okada M
  • Journal Title: J Thorac Cardiovasc Surg Volume: 144(6) Pages: 1365-71
  • Peer Reviewed
• [Journal Article] Use of high-resolution computed tomography and positron emission tomography/computed tomography in the management of stage IA adenocarcinoma (2012)
  • Author(s): Miyata Y, Tsutani Y, Okada M
  • Journal Title: Semin Thorac Cardiovasc Surg Volume: 24(4) Pages: 267-74
  • Peer Reviewed
• [Journal Article] Prognostic significance of using solid versus whole tumor size on high-resolution computed tomography for predicting pathologic malignant grade of tumors in clinical stage IA lung adenocarcinoma: a multicenter study (2012)
  • Author(s): Tsutani Y, Miyata Y, Nakayama H, Okumura S, Adachi S, Yoshimura M, Okada M
  • Journal Title: J Thorac Cardiovasc Surg Volume: 143(3) Pages: 607-12
  • Peer Reviewed
• [Journal Article] Prediction of pathologic node-negative clinical stage IA lung adenocarcinoma for optimal candidates undergoing sublobar resection (2012)
  • Author(s): Tsutani Y
  • Journal Title: J Thorac Cardiovasc Surg Volume: 144 Issue: 6 Pages: 1365-1371
  • DOI: 10.1016/j.jtcvs.2012.07.012
  • Peer Reviewed
• [Journal Article] Notch2 and Six1 are up-regulated during progression of early-stage lung adenocarcinoma and define its unfavorable subset at advanced stages. (2012)
  • Author(s): Mimae T, Murakami Y, Ito A et al.
  • Journal Title: Clinical Cancer Research Volume: 18 Issue: 4 Pages: 945-948
  • DOI: 10.1158/1078-0432.ccr-11-1946
  • Peer Reviewed
• [Journal Article] Prognostic significance of using solid versus whole tumor size on high-resolution computed tomography for predicting pathologic malignant grade of tumors in clinical stage IA lung adenocarcinoma: a multicenter study (2012)
  • Author(s): Tsutani Y
  • Journal Title: J Thorac Cardiovasc Surg Volume: 143 Issue: 3 Pages: 607-612
  • DOI: 10.1016/j.jtcvs.2011.10.037
  • Peer Reviewed
• [Journal Article] Prognostic significance of using solid versus whole tumor size on high-resolution computed tomography for predicting pathologic malignant grade of tumors in clinical stage IA lung adenocarcinoma : a multicenter study (2012)
  • Author(s): Tsutani Y, Miyata Y, Nakayama H, Okumura S, Adachi S, Yoshimura M, Okada M
  • Journal Title: J Thorac Cardiovasc Surg Volume: 143 Pages: 607-612
  • Peer Reviewed
• [Journal Article] Difference in prognostic significance of maximum standardized uptake value on [18F]-fluoro-2-deoxyglucose positron emission tomography between adenocarcinoma and squamous cell carcinoma of the lung (2011)
  • Author(s): Tsutani Y, Miyata Y, Misumi K, Ikeda T, Mimura T, Hihara J, Okada M
  • Journal Title: Jpn J Clin Oncol Volume: 41 Pages: 890-896
  • Peer Reviewed
• [Journal Article] Multicenter analysis of high-resolution computed tomography and positron emission tomography/computed tomography findings to choose therapeutic strategies for clinical stage IA lung adenocarcinoma (2011)
  • Author(s): Okada M, Nakayama H, Okumura S, Daisaki H, Adachi S, Yoshimura M, Miyata Y
  • Journal Title: J Thorac Cardiovasc Surg Volume: 141 Pages: 1384-1391
  • Peer Reviewed
• [Presentation] Expression of HIF1αand GLUT1 correlates with FDG uptake on PET and tumor aggressiveness in different histologic subtypes of lung adenocarcinomas (2012)
  • Author(s): Miyata Y, Furukawa T, Tsutani Y, Mimae T, Misumi K, Yoshiya T, Ibuki Y, Okada M
  • Organizer: ESMO2012
  • Place of Presentation: Vienna, Austria
• [Presentation] Expression of HIF1α and GLUT1 correlates with FDG uptake on PET and tumor aggressiveness in different histologic subtypes of lung adenocarcinomas (2012)
  • Author(s): Miyata Y
  • Organizer: ESMO
  • Place of Presentation: Vienna, Austria
• [Presentation] A Multicenter Analysis of High-resolution Computed Tomograpliy and Fluorodeoxyglucose-positron Emission Tomography/computed Tomography to Predict Malignant Grade of Clinical Stage Ia Lung Adenocarcinoma (2011)
  • Author(s): Miyata Y, Okada M, Okumura H, Daisaki S, Adachi S, Yoshimura H, Nakayama K
  • Organizer: The 2011 European Multidisciplinary Cancer Congress (ESMO 36)
  • Place of Presentation: Stockholm, Sweden
  • Year and Date: 2011-09-25
• [Remarks]
  • URL: http://home.hiroshima-u.ac.jp/genge/