Role of astrocytes in cerebral microcirculation -observation with two photon intravital microscope-

• Project/Area Number: 22591597
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Cerebral neurosurgery
• Research Institution: Jichi Medical University
• Principal Investigator: ISHIKAWA Mami 自治医科大学, 医学部, 准教授 (60212859)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
• Keywords: アストロサイト / 脳虚血再灌流 / くも膜下出血 / 一酸化炭素 / 硫化水素 / 脳血管 / 脳循環 / 脳梗塞 / 蛍光画像 / 血管透過性 / GFAP

Research Abstract

GFP-labeled astrocytes and Qdot-labeled arterioles and capillaries were observed with two photon intravital microscope in GFAP-GFP mice. Penetrating arterioles and precapillary arterioles regulate cerebral microcirculation in mice with an infarct or a sub

Research Products

• [Journal Article] Hypoxic regulation of the cerebral microcirculation is mediated by a carbon monoxide-sensitive hydrogen sulfide pathway (2012)
  • Author(s): Morikawa T, Kajimura M, Nakamura T, Hishiki T, Nakanishi T, Yukutake Y, Nagahata Y, Ishikawa M, Hattori K, Takenouchi T, Takahashi T, Ishii I, Matsubara K, Kabe Y, Uchiyama S, Nagata E, Gadalla MM, Snyder SH, Suematsu M
  • Journal Title: PNAS Volume: 109 Pages: 1293-1298
• [Journal Article] Acute CO2-independent vasodilatation of penetrating and pre-capillary arterioles in mouse cerebral parenchyma upon hypoxia revealed by a thinned-skull window method (2011)
  • Author(s): Nakamura T, Kajimura M, Morikawa T, Hattori K, Ishikawa M, Yukutake Y, Uchiyama S-I, and Suematsu M
  • Journal Title: Acta Physiol. Volume: 203 Pages: 187-196
• [Presentation] くも膜下出血直後の脳微小循環 GFAP-GFP マウスを用いた2光子励起生体蛍光顕微鏡による観察 (2013)
  • Author(s): 石川眞実、加持春菜、海老原彰、 草鹿元、田中裕一、森川隆之、中村智実、梶村眞弓、末松誠
  • Organizer: Stroke2013(日本脳卒中の外科学会)
  • Place of Presentation: 横浜
  • Year and Date: 2013-03-23
• [Presentation] 脳主幹動脈閉塞時の脳循環代謝予備能の解析 (2013)
  • Author(s): 石川眞実、加持春菜、海老原彰、 草鹿元、田中裕一、森川隆之、中村智実、梶村眞弓、末松誠
  • Organizer: Stroke2013 (日本脳卒中学会)
  • Place of Presentation: 横浜
  • Year and Date: 2013-03-21
• [Presentation] Cerebral arteriolar responses immediately after MCAO and reperfusion (2013)
  • Author(s): M Ishikawa, M Kajimura, T Morikawa, T Nakamura, H Kamochi, A Ebihara, G Kusaka, Y Tanaka, M Suematsu
  • Organizer: The 38 Annual Meeting of Japanese Sicuett for Micorcirculation Tokyo
  • Year and Date: 2013-02-08
• [Presentation] Cerebral arteriolar responses immediately after MCAO and reperfusion (2013)
  • Author(s): 石川眞実
  • Organizer: 第３８回日本微小循環学会
  • Place of Presentation: 東京
• [Presentation] 脳主幹動脈閉塞時の脳循環代謝予備能の解析 (2013)
  • Author(s): 石川眞実
  • Organizer: Stroke2013
  • Place of Presentation: 横浜
• [Presentation] GFAP-GFP マウスの脳微小循環とアストロサイトの観察 2 光子励起生体蛍光顕微鏡を用いて (2012)
  • Author(s): 石川眞実、加持春菜、海老原彰、草鹿元、田中裕一、森川隆之、中村智実、梶村眞弓、末松 誠
  • Organizer: 第24回日本脳循環代謝学会総会
  • Place of Presentation: 広島
  • Year and Date: 2012-11-09
• [Presentation] 内頚動脈・中大脳動脈閉塞時の患側健側脳循環予備能の解析 (2012)
  • Author(s): 石川眞実、伊古田雅史、山黒友丘、加持春菜、海老原彰、草鹿 元、田中裕一
  • Organizer: 第24回日本脳循環代謝学会総会
  • Place of Presentation: 広島
  • Year and Date: 2012-11-08
• [Presentation] くも膜下出血直後の脳微小循環とアストロサイト2 光子励起生体蛍光顕微鏡によるマウスでの観察 (2012)
  • Author(s): 石川眞実、加持春菜、海老原彰、草鹿元、田中裕一、森川隆之、中村智実、梶村眞弓、末松 誠
  • Organizer: 第71回日本脳神経外科学会総会
  • Place of Presentation: 大阪
  • Year and Date: 2012-10-18
• [Presentation] 内頚動脈・中大脳動脈閉塞時の患側健側脳循環予備能の解析 (2012)
  • Author(s): 石川眞実
  • Organizer: 第２４回日本脳循環代謝学会総会
  • Place of Presentation: 広島
• [Presentation] GFAP-GFPマウスの脳微小循環とアストロサイトの観察 2光子励起生体蛍光顕微鏡を用いて (2012)
  • Author(s): 石川眞実
  • Organizer: 第２４回日本脳循環代謝学会総会
  • Place of Presentation: 広島
• [Remarks] 自治医科大学研究シーズ
  • URL: http://rseeds.jichi.ac.jp/research_seeds/admin/ResearchResultList.php?publicId=14opE7ws6jbhTHDMJfccJbIQj6kTVF