The effect of anti-angiogenic therapy and antibody therapy for distant metastasis in osteosarcoma.

• Project/Area Number: 22591672
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Aichi Cancer Center Research Institute
• Principal Investigator: SUGIURA Hideshi 愛知県がんセンター(研究所), 腫瘍免疫学部, 研究員 (50303615)
• Co-Investigator(Kenkyū-buntansha): KYOGASHIMA Mamoru 日本薬科大学, 薬学部, 教授 (50225091)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 骨 / 軟部腫瘍 / 抗IL-2α抗体 / 抗IL-2抗体 / 制御性T細胞 / FACS / LM8 / 骨肉腫 / 肺転移 / マウス / 肝転移 / Foxp3 / 抗IL2レセプタα単抗体 / 抗IL2単抗体 / 抑制性T細胞

Research Abstract

We evaluated the antitumor activity of anti-interleukin 2 receptor (IL2R) α(CD25) monoclonal antibody (PC61) or an anti-interleukin-2 monoclonal antibody (S4B6) in a murine osteosarcoma model (LM8). Tumors size and the numbers of lung and liver metastatic colonies were significantly decreased in both PC61 and S4B6 groups. Flow cytometry revealed that mouse regulatory T cells (Treg) can be deleted by intraperitoneal injection of PC61 or S4B6. Next, we examined the effectiveness of PC61 or S4B6 combined with Candesartan, but there were significantly no differences. It was suggested that PC61 and S4B6 strongly will suppress metastases and tumor progression.

Research Products

• [Journal Article] Antitumor activity of an interleukin-2 monoclonal antibody in a murine osteosarcoma transplantation model. (2012)
  • Author(s): Kohyama K, Sugiura H, Kozawa E, Wasa J, Yamada K, Nishioka A, Kamei Y, Taguchi O
  • Journal Title: Anticancer Res Volume: 32(3) Pages: 779-82
  • URL: http://ar.iiarjournals.org/content/32/3/779.full.pdf+html
• [Journal Article] Antitumor activity of an interleukin-2 monoclonal antibody in a murine osteosarcoma transplantation model (2012)
  • Author(s): Kohyama K
  • Journal Title: ANTICANCER RESEARCH Volume: 32（3） Pages: 779-782
  • Peer Reviewed
• [Journal Article] The tumor suppressive effect of angiotensin II type 1 receptor antagonist in a murine osteosarcoma model. (2011)
  • Author(s): Wasa J, Sugiura H, Kozawa E, Kohyama K, Yamada K, Taguchi O
  • Journal Title: Anticancer Res. Volume: 31(1) Pages: 123-7
  • URL: http://ar.iiarjournals.org/content/31/1/123.full.pdf+html
• [Journal Article] Suppression of Tumour Metastasis in a Murine Osteosarcoma Model with Anti-CD25 Monoclonal Antibody Treatment (2010)
  • Author(s): Kozawa E, Sugiura H, Wasa J, Kohyama K, Yamada K, Nishioka A, Nishida Y, Ishiguro N, Taguchi O
  • Journal Title: Anticancer Res Volume: 30 Pages: 5019-5022
  • URL: http://ar.iiarjournals.org/content/30/12/5019.full.pdf+html
• [Journal Article] Suppression of tumour metastasis in a murine osteosarcoma model with anti-CD25 monoclonal antibody treatment (2010)
  • Author(s): Kozawa E
  • Journal Title: ANTICANCER RESEARCH Volume: 30巻 Pages: 5019-5022
  • Peer Reviewed
• [Journal Article] Multicenter phase II trial assessing effectiveness of imatinib mesylate on relapsed or refractory KIT-positive or PDGFR-positive sarcoma (2010)
  • Author(s): Sugiura H
  • Journal Title: Journal of Orthopaedic Science Volume: 15巻 Pages: 654-660
  • NAID: 10027872091
  • Peer Reviewed
• [Presentation] Tumor Suppression with Anti-Interleukin-2 and Anti-CD25 Monoclonal Antibodies in a Murine Osteosarcoma Model (2012)
  • Author(s): Sugiura H, Kozawa E, Kohyama K, Yamada K, Nishida Y, Taguchi O
  • Organizer: 9^<TH> Asia Pacific Musculoskeletal Tumour Sosiety Meeting
  • Place of Presentation: ヒルトン・クアラルンプール(クアラルンプール・マレーシア)
• [Presentation] Tumor suppression with anti-Interleukin-2 and anti-CD25 monoclonal antibodies in a murine osteosarcoma model (2012)
  • Author(s): Sugiura H
  • Organizer: 9TH ASIA PACIFIC MUSCULOSKELETAL TUMOUR SOCIETY MEETING
  • Place of Presentation: ヒルトン クアラルンプール (クアラルンプール)
• [Presentation] Tumor-suppressive Effect of Anti-interleukin-2 Monoclonal Antibody in a Murine Osteosarcoma Model (2011)
  • Author(s): Kohyama K, Sugiura H, Taguchi O, Kozawa E, Wasa J, Yamada K, Nishida T, Kamei Y
  • Organizer: I ISOLS 2011 16^<TH> General Meeting
  • Place of Presentation: 北京(中国)
• [Presentation] Tumor-suppressive Effect of Anti-interleukin-2 Monoclonal Antibody in a Murine Osteosarcoma Model (2011)
  • Author(s): Kohyama K
  • Organizer: ISOLS 2011 16^<TH> GENERAL MEETING
  • Place of Presentation: National Convention Center(北京)