Epigenetics of mesenchymal stem cells considering regenerative therapy for osteoarthritis

• Project/Area Number: 22591681
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: EZURA Yoichi 東京医科歯科大学, 難治疾患研究所, 准教授 (50333456)
• Co-Investigator(Renkei-kenkyūsha): SEKIYA Ichiro 東京医科歯科大学, 大学院・医歯学研究科, 教授 (10345291)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 間葉系幹細胞 / 軟骨細胞分化 / エピジェネティクス / 遺伝子 / 再生医学 / シトシン・メチル化

Research Abstract

The differentiating potentials of human mesenchymal stem cells (hMSCs) were analyzed from epigenetic aspect. By analyzing cytosine methylation status of various gene loci, an upstream region of stromal derived factor 1 (also called as CSCL12) gene was hypothesized to function as silencer element of this gene in hMSCs. Although the alteration of cytosine methylation was a rare event in the in vitro differentiation system, global analysis indicated consistent differences could be represented among hMSCs with different tissue origins, which may reflect relatively long term epigenetic history of individual cells.

Research Products

• [Presentation] Identification of Signature Genes Selectively Expressed in Mesenchymal Stem Cells Derived from Synovial JointTissues (Poster Sessions) (2012)
  • Author(s): Ezura Y, Hayata T, Nakamoto T, Notomi T, Muneta T, Sekiya I, Noda M
  • Organizer: 2012 ASBMRannual meeting
  • Place of Presentation: Minneapolis, USA
• [Presentation] ヒト骨髄および滑膜由来間葉系細胞において異なるCpGメチル化を示す遺伝子群の探索と骨軟骨細胞分化の制御に関わる転写因子群の抽出:RUNX2およびRUNX3,DLX5,ALX4遺伝子 (2012)
  • Author(s): 江面陽一、早田匡芳、中元哲也、納富拓也、 関矢一郎 、宗田大、野田政樹
  • Organizer: 第30回日本骨代謝学会
  • Place of Presentation: 東京
• [Presentation] Identification of Signature Genes Selectively Expressed in Mesenchymal Stem Cells Derived from Synovial Joint Tissues (2012)
  • Author(s): 江面陽一
  • Organizer: 米国骨代謝学会
  • Place of Presentation: ミネアポリス（アメリカ合衆国）
• [Presentation] ヒト骨髄および滑膜由来間葉系細胞において異なるＣｐＧメチル化を示す遺伝子群の探索と骨軟骨細胞分化の制御に関わる転写因子群の抽出 (2012)
  • Author(s): 江面陽一
  • Organizer: 日本骨代謝学会
  • Place of Presentation: 東京
• [Presentation] Identification of the Differentially Methylated Promoter and Intragenic CpG-Cytosines in the Human Synovium and Bone Marrow Derived Mesenchymal stem Cells (2011)
  • Author(s): 江面陽一, ほか6名
  • Organizer: 米国骨代謝学会
  • Place of Presentation: サンディエゴ(米国)
  • Year and Date: 2011-09-19
• [Presentation] Identification of theDifferentially Methylated Promoter and Intragenic CpG-Cytosines in the Human Synovium and Bone Marrow Derived Mesenchymal Stem Cells (Poster Sessions) (2011)
  • Author(s): Yoichi Ezura, Tadayoshi Hayata, Tetsuya Nakamoto, Takuya Notomi, Ichiro Sekiya Takeshi Muneta, Masaki Noda
  • Organizer: The 33^<rd> Annual Meeting of American Society for Bone and Mineral Research
  • Place of Presentation: San Diego, California, USA