Induction of chondrocyte from human induced pluripotent stem cells

• Project/Area Number: 22591693
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Kinki University
• Principal Investigator: FUKUDA Kanji 近畿大学, 医学部, 教授 (50201744)
• Co-Investigator(Kenkyū-buntansha): NISHIO Kazuto 近畿大学, 医学部, 教授 (10208134) ; TERAMURA Takeshi 近畿大学, 医学部, 講師 (40460901)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 軟骨 / iPs細胞 / 分化誘導 / 再生医療 / iPS細胞

Research Abstract

Induction of chondrocyte from iPS cells is an important to treat osteoarticular disorders. We performed 1) induction of the chondrocyte from iPS cells via MSC production from iPS cells; 2) detailed characterization of the MSCs; 3) observation of cellular responses to mechanical stresses. These experiments elucidated that 1) hypoxic treatment during in vitro differentiation is a positive factor for induction of the MSCs, 2) induced MSCs possess typical MSC characteristics and 3) loading of the mechanical stress induce decreasing of the genes controlling undifferenciency of the cells. The present series of studies suggest an effective method to furnish the chondrocyte from iPS cells.

Research Products

• [Journal Article] Generation of Embryonic Stem Cell Lines from Immature Rabbit Ovarian Follicles (2013)
  • Author(s): Teramura T, Sugimoto H, Frampton J, Kida Y, Nakano M, Kawakami M, Izumi H, Fukunaga N, Onodera Y, Takehara T, Fukuda K, Hosoi Y
  • Journal Title: Stem Cells Dev Volume: 22(6) Pages: 928-38
  • Peer Reviewed
• [Journal Article] Induction of Functional Mesenchymal Stem Cells from Rabbit Embryonic Stem Cells by Exposure to Severe Hypoxic Conditions (2013)
  • Author(s): Teramura T, Onodera Y, Takehara T, Frampton J, Matsuoka T, Ito S, Nakagawa K, Miki Y, Hosoi Y, Hamanishi C, Fukuda K
  • Journal Title: Cell Transplant Volume: 22(2) Pages: 309-29
  • Peer Reviewed
• [Journal Article] Cyclic compression-induced p38 activation and subsequent MMP13 expression requires Rho/ROCK activity in bovine cartilage explants (2012)
  • Author(s): Nakagawa K, Teramura T, Takehara T, Onodera Y, Hamanishi C, Akagi M Fukuda K
  • Journal Title: InflammRes Volume: 61(10) Pages: 1093-100
  • Peer Reviewed
• [Journal Article] Mechanical stimulation of cycli tensile strain induces reduction o pluripotent related gene expressions via activation of Rho/ROCK and subsequent decreasing of AKT phosphorylation in human induced pluripotent stem cells (2012)
  • Author(s): Teramura T, Takehara T, Onodera Y, Nakagawa K, Hamanishi C, Fukuda K
  • Journal Title: Biochem Biophys Res Commun Volume: 417(2) Pages: 836-41
  • Peer Reviewed
• [Journal Article] Reduced Oxygen Concentration Enhances Conversion of Embryonic Stem Cells to Epiblast Stem Cells (2012)
  • Author(s): Takehara T, Teramura T, Onodera Y, Hamanishi C, Fukuda K
  • Journal Title: Stem CellsDev Volume: 21(8) Pages: 1239-49
  • Peer Reviewed
• [Journal Article] Mechanical stimulation of cyclic tensile strain induces reduction of pluripotent related gene expressions via activation of Rho/ROCK and subsequent decreasing of AKT phosphorylation in human induced pluripotent stem cells (2012)
  • Author(s): Teramura T
  • Journal Title: Biochem Biophys Res Commun Volume: 417 Pages: 836-841
  • Peer Reviewed
• [Journal Article] Leukemia inhibitory factor (LIF) enhances germ cell differentiation from primate embryonic stem cells (2010)
  • Author(s): Fukunaga N, Teramura T, Onodera Y, Takehara T, Fukuda K, Hosoi Y
  • Journal Title: Cell Reprogram Volume: 12(4) Pages: 369-76
  • Peer Reviewed
• [Journal Article] Induction of mesenchymal progenitor cells with chondrogenic property from mouse-induced pluripotent stem cells (2010)
  • Author(s): 寺村岳士, 小野寺勇太, 三原敏敬, 細井美彦, 浜西千秋, 福田寛二
  • Journal Title: Cellular Reprogramming Volume: 21 Pages: 249-261
  • Peer Reviewed
• [Presentation] 人工多能性幹(iPS)細胞からの間葉系幹細胞の分化誘導 (2010)
  • Author(s): 寺村岳士, 小野寺勇太, 浜西千秋, 福田寛二
  • Organizer: 第25回日本整形外科基礎学術集会
  • Place of Presentation: 京都国際会議場
  • Year and Date: 2010-10-15
• [Presentation] メカニカルストレスはRho/ROCKの活性化を介してヒトiPS細胞の未分化性制御に関与する
  • Author(s): 小野寺 勇太 他3名
  • Organizer: 日本整形外科学会基礎学術集会
  • Place of Presentation: 名古屋国際会議場
• [Presentation] 低酸素培養によるヒトiPS細胞からの間葉系幹細胞の分化誘導
  • Author(s): 寺村 岳士 他3名
  • Organizer: 日本再生医療学会
  • Place of Presentation: パシフィコ横浜
• [Remarks]
  • URL: http://www.med.kindai.ac.jp/stemcell/
• [Patent(Industrial Property Rights)] 免疫不全動物を用いた細胞の製 (2010)
  • Inventor(s): 寺村岳士、福田寛二
  • Industrial Property Rights Holder: 学校法人近畿大学
  • Filing Date: 2010-12-08
  • Overseas
• [Patent(Industrial Property Rights)] 免疫不全動物を用いた細胞の製法 (2010)
  • Inventor(s): 寺村岳士, 福田寛二
  • Industrial Property Rights Holder: 学校法人近畿大学
  • Industrial Property Number: 2010-274147
  • Filing Date: 2010-12-08
  • Overseas