Development of novel diagnostic tool for male infertility using near-infrared spectroscopy

• Project/Area Number: 22591807
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Urology
• Research Institution: Osaka University
• Principal Investigator: MIYAGAWA Yasushi 大阪大学, 大学院・医学系研究科, 講師 (70362704)
• Co-Investigator(Kenkyū-buntansha): 辻村 晃 大阪大学, 大学院・器官制御外科学, 泌尿器科 (40294053)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 男性不妊症 / 精液検査 / 精子 / 精巣 / 近赤外分光法

Research Abstract

Non-obstructive azoospermia (NOA) is an vital condition of male infertility which presents no sperm in ejaculate. But 40% of NOA patients still maintainspermatogenesis in their testis and testicular sperm extraction combined with intracytoplasmic sperm injection is only the way to treat NOA patients. However it is actually difficult to predict successful sperm retrieval presurgically by previous diagnostic tools. We found near-infrared spectroscopic examination may predict existence of sperm in non-invasive condition. Further study will help us to predict successful outcome more accurately .

Research Products

• [Journal Article] A single nucleotide polymorphism within the novel sex-linked testis-specific retrotransposed PGAM4 gene influences human male fertility (2012)
  • Author(s): Okuda H, Tsujimura A, Irie S, Yamamoto K, Fukuhara S, Matsuoka Y, Takao T, Miyagawa Y, Nonomura N, Wada M, Tanaka H
  • Journal Title: PLoS One Volume: 7
• [Journal Article] Single-nucleotide polymorphisms in HORMAD1 may be a risk factor for azoospermia caused by meiotic arrest in Japanese patients (2012)
  • Author(s): Miyamoto T, Tsujimura A, Miyagawa Y, Koh E, Namiki M, Horikawa M, Saijo Y, Sengoku K
  • Journal Title: Asian J Androl Volume: 14 Pages: 580-3
  • NAID: 120006580771
• [Journal Article] Single nucleotide polymorphisms in the SEPTIN12 gene may be associated with azoospermia by meiotic arrest in Japanese men (2012)
  • Author(s): Miyamoto T, Tsujimura A, Miyagawa Y, Koh E, Namiki M, Horikawa M, Saijo Y, Sengoku K
  • Journal Title: J Assist Reprod Genet Volume: 29 Pages: 47-51
• [Journal Article] Male infertility and its causes in human (2012)
  • Author(s): Miyamoto T, Tsujimura A, Miyagawa Y, Koh E, Namiki M, Sengoku K
  • Journal Title: Adv Urol. Volume: 2012 Pages: 384520-384520
• [Journal Article] Single-nucleotide polymorphisms in the SEPTIN12 gene may be a genetic risk factor for Japanese patients with Sertoli cell-only syndrome (2012)
  • Author(s): Miyakawa H, Miyamoto T, Koh E, Tsujimura A, Miyagawa Y, Saijo Y, Namiki M, Sengoku K.
  • Journal Title: J Androl Volume: 33 Pages: 483-7
• [Journal Article] 乏精子症・無精子症に対する薬物療法.男性サイドからみる生殖医療の最前線、 (2012)
  • Author(s): 辻村 晃, 宮川 康, 野々村 祝夫
  • Journal Title: 日本医師会雑誌 Volume: 141 Pages: 1241-1244
• [Journal Article] 精巣内精子採取術時と顕微授精時における精子の評価 (2012)
  • Author(s): 岡本 吉夫, 吉住 彩香, 一谷 有希子, 土家 真紀, 高尾 徹也, 宮川 康, 高田 晋吾, 松宮 清美, 辻村 晃
  • Journal Title: 泌尿器外科 Volume: 2 Pages: 91-395
• [Journal Article] Morphologic and mitochondrial characterization of human spermatogenic cells dispersed in wet preparation for testicular sperm extraction: establishment of a microscopic diagram of developing human spermatogenic cells (2011)
  • Author(s): Okuda H, Tsujimura A, Yamamoto K, Fukuhara S, Nakayama J, Kiuchi H, Takao T, Miyagawa Y, Nonomura N, Okuyama A
  • Journal Title: Fertil Steril Volume: 95 Pages: 2665-8
• [Journal Article] Single nucleotide polymorphism in the UBR2 gene may be a genetic risk factor for Japanese patients with azoospermia by meiotic arrest (2011)
  • Author(s): Miyamoto T, Tsujimura A, Miyagawa Y, Koh E, Namiki M, Horikawa M, Saijo Y, Sengoku K
  • Journal Title: J Assist Reprod Genet. Volume: 28 Pages: 743-6
• [Journal Article] Morphologic and mitochondrial characterization of human spennatogenic cells dispersed in wet preparation for testicular sperm extraction : establishment of a microscopic diagram of developing human spermatogenic cells (2011)
  • Author(s): Okuda H, Tsujimura A, Yamamoto K, Fukuhara S, Nakayama J, Kiuchi H, Takao T, Miyagawa Y, Nonomura N, Okuyama A
  • Journal Title: Fertil Steril Volume: 95 Issue: 8 Pages: 1665-8
  • DOI: 10.1016/j.fertnstert.2011.04.026
  • Peer Reviewed
• [Journal Article] Single nucleotide polymorphism in the UBR2 gene may be a genetic risk factor for Japanese patients with azoospermia by meiotic airest (2011)
  • Author(s): Miyamoto T, Tsujimura A, Miyagawa Y, Koh E, Namiki M, Horikawa M, Saijo Y, Sengoku K
  • Journal Title: J Assist Reprod Genet Volume: 28 Issue: 8 Pages: 743-6
  • DOI: 10.1007/s10815-011-9576-y
  • Peer Reviewed
• [Journal Article] Single-Nucleotide Polymorphisms in the SEPTIN12 Gene May Be a Genetic Risk Factor for Japanese Patients with Sertoli Cell-Only Syndrome (2011)
  • Author(s): Miyakawa H, Miyamoto T, Koh E, Tsujimura A, Miyagawa Y, Saijo Y, Namiki M, Sengoku K
  • Journal Title: J Androl Volume: (Epub ahead of print) Issue: 3 Pages: 483-487
  • DOI: 10.2164/jandrol.110.012146
  • Peer Reviewed
• [Journal Article] Current concepts of the human azoospermia and its causes (2010)
  • Author(s): Miyamoto T, Tsujimura A, Miyagawa Y, Koh E, Namiki M, Sengoku K
  • Journal Title: Biol Med Reprod Volume: 9 Pages: 121-127
  • NAID: 10027050484
• [Journal Article] Current concepts of the human azoospermia and its causes. (2010)
  • Author(s): Miyamoto T, Tsujimura A, Miyagawa Y, Koh E, Namiki M, Sengoku K
  • Journal Title: Biol Med Reprod Volume: 9 Pages: 121-127
  • NAID: 10027050484
  • Peer Reviewed
• [Journal Article] Alterations in the steroid hormone receptor co-chaperone FKBPL are associated with male infertility : a case-control study. (2010)
  • Author(s): Sunnotel O, Hiripi L, Lagan K, McDaid JR, De Leon JM, Miyagawa_Y, Crowe H, Kaluskar S, Ward M, Scullion C, Campbell A, Downes CS, Hirst D, Barton D, Mocanu E, Tsujimura A, Cox MB, Robson T, Walsh CP
  • Journal Title: Reproductive Biology and Endocrinology Volume: 8(On line)
  • Peer Reviewed
• [Journal Article] Single Nucleotide Polymorphisms of the IZUMO Gene in Male Infertile Patients. (2010)
  • Author(s): Irie S, Nakamura J, Tsujimura A, Miyagawa Y, Tanaka H
  • Journal Title: Nagasaki Int.Univ.Rev. Volume: 10 Pages: 209-213
  • NAID: 120005578649
  • Peer Reviewed
• [Presentation] 非モザイク型クラインフェルター症候群に対する Micro-TESE の成績 (2012)
  • Author(s): 宮川 康, 竹澤 健太郎, 奥田 英伸, 木内 寛, 高尾 徹也, 辻村 晃, 松岡 庸洋, 高田 晋吾, 松宮 清美, 野々村 祝夫
  • Organizer: 第57回日本 生殖医 学会 総会
  • Place of Presentation: 長崎市
  • Year and Date: 2012-11-08
• [Presentation] 精巣内精子採取術 (2011)
  • Author(s): 宮川 康、奥田英伸、山本圭介、福原慎一郎、吉岡 巌、木内 寛、高尾徹也、辻村 晃、野々村祝夫
  • Organizer: 第16回男性不妊症手術フォーラム
  • Place of Presentation: 横浜市
  • Year and Date: 2011-12-09
• [Presentation] 精巣内精子採取術 (2011)
  • Author(s): 宮川康
  • Organizer: 第16回男性不妊症手術フォーラム
  • Place of Presentation: 神奈川県
  • Year and Date: 2011-12-09
• [Presentation] Clinical investigation of patients with ejaculatory dysfunction and male infertility (2011)
  • Author(s): Yasushi Miyagawa, Tetsuya Takao, Hidenobu Okuda, Keisuke Yamamoto, Yasuhiro Matsuoka, Akira Tsujimura, Shingo Takada, Kiyomi Matsumiya, Yoshio Okamoto , Norio Nonomura
  • Organizer: The 13thBiennial Meeting of the Asia
  • Place of Presentation: Pacific Society for Sexual MedicineKaohsiung, Taiwan
  • Year and Date: 2011-11-18
• [Presentation] 男性不妊症患者におけるCalsperin(CALR3)遺伝子の一塩多型の探索 (2011)
  • Author(s): 宮川康
  • Organizer: 第30回日本アンドロロジー学会
  • Place of Presentation: 東京都
  • Year and Date: 2011-07-23
• [Presentation] 大阪大学医学部附属病院・生殖医療センターの開設3年間の男性不妊診療実績 (2011)
  • Author(s): 宮川康
  • Organizer: 第99回日本泌尿器科学会
  • Place of Presentation: 愛知県(招待講演)
  • Year and Date: 2011-04-23
• [Presentation] 男性不妊症患者におけるIZUMO遺伝子の一塩基多型 (2010)
  • Author(s): 宮川康、奥田英伸、山本圭介、福原慎一郎、高尾徹也、辻村晃、野々村祝夫、奥山明彦、入江新司、中村順子、田中宏光
  • Organizer: 第55回日本生殖医学会
  • Place of Presentation: 徳島市
  • Year and Date: 2010-11-12
• [Presentation] 大阪大学医学部附属病院・生殖医療センターの開設 3 年間の男性不妊診療実績 (2010)
  • Author(s): 宮川 康、奥田英伸、山本圭介、福原慎一郎、高尾徹也、辻村 晃、野々村祝夫、筒井建紀、木村 正、奥山明彦
  • Organizer: 第41回関西アンドロロジーカンファレンス
  • Place of Presentation: 大阪市
  • Year and Date: 2010-10-02
• [Presentation] 大阪大学医学部附属病院・生殖医療センターの開設3年間の男性不妊診療実績 (2010)
  • Author(s): 宮川康、奥田英伸、山本圭介、福原慎一郎、高尾徹也、辻村晃、野々村祝夫、筒井建紀、木村正、奥山明彦
  • Organizer: 第41回関西アンドロロジーカンファレンス
  • Place of Presentation: 大阪市
  • Year and Date: 2010-10-02
• [Presentation] 男性不妊患者に検出される IZUMO 遺伝子の一塩基多型 (2010)
  • Author(s): 宮川 康、奥田英伸、山本圭介、福原慎一郎、高尾徹也、辻村 晃、野々村祝夫、奥山明彦、入江新司、中村順子、田中宏光
  • Organizer: 第29回日本アンドロロジー学会
  • Place of Presentation: 神戸市
  • Year and Date: 2010-07-30
• [Book] 男性因子に対する治療、 1)薬物療法 生殖医療ガイドブック 2010 (2010)
  • Author(s): 辻村 晃、宮川 康
  • Publisher: 金原出版株式会社