The effect of estrogen for carbohydrate metabolism in pancreatic beta cells

• Project/Area Number: 22591853
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Obstetrics and gynecology
• Research Institution: Shinshu University
• Principal Investigator: NISHIO Shin-ichi 信州大学, 医学部附属病院, 講師 (30467146)
• Co-Investigator(Kenkyū-buntansha): KOMATSU Mitsuhisa 信州大学, 医学部, 教授 (90221978)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: エストロゲン / 膵β細胞 / 転写調節因子 / インスリンプロモーター / 膣β組胞 / 転写調節子

Research Abstract

We have demonstrated the treatment of HIT-T15 insulinoma cellswith E2 led to the decreased insulin mRNA levels. Transient transfection assays of HIT-T15 cells revealed that ERα repressed transcriptional activity of rat insulin II promoter bothin a ligand-dependent and -independent manner. The repression was not mediated by an ERE-like sequence or AP-1 site in the promoter. Instead, transcriptional factor PDX-1 and BETA2 appear to be involved.

Research Products

• [Journal Article] Possible Roles of the AP-1 Site in the Cytosolic T3 Binding Protein Promoter and Insights into its Physiological Significance. (2013)
  • Author(s): Suzuki S, Nishio SI, Ishii H, Sekido T, Takeshige K,Ohkubo Y, Hiwatashi D, Takeda T, Komatsu M.
  • Journal Title: Horm Metab Res. Volume: Mar 18. Issue: 07 Pages: 501-506
  • DOI: 10.1055/s-0033-1337933
  • Peer Reviewed
• [Journal Article] A new experimental model of ATP-sensitive K⁺ channel-independent insulinotropic action of glucose: a permissive role of cAMP for triggering of insulin release from rat pancreatic β-cells (2013)
  • Author(s): Masahiro Takei
  • Journal Title: Endocrine Journal Volume: 60 Issue: 5 Pages: 599-607
  • DOI: 10.1507/endocrj.EJ12-0388
  • NAID: 10031184527
  • ISSN: 0918-8959, 1348-4540
  • Peer Reviewed
• [Journal Article] Humoral hypercalcemia associated with gastric carcinoma secreting parathyroid hormone: a case report and review of the literature (2013)
  • Author(s): Nakajima K, Tamai M, Okaniwa S, Nakamura Y, Kobayashi M, Niwa T, Horigome N, Ito N, Suzuki S, Nishio S, Komatsu M.
  • Journal Title: Endocrine Journal Volume: 60 Issue: 5 Pages: 557-562
  • DOI: 10.1507/endocrj.EJ12-0406
  • NAID: 10031184522
  • ISSN: 0918-8959, 1348-4540
  • Peer Reviewed
• [Journal Article] Fasting induces CART down-regulation in the zebrafish nervous system in a cannabinoid receptor 1-dependent manner. (2012)
  • Author(s): Nishio S, Gibert Y, Berekelya L, Bernard L, Brunet F, Guillot E, Le Bail JC, Sánchez JA, Galzin AM, Triqueneaux G, Laudet V.
  • Journal Title: Mol Endocrinol. Volume: 26(8) Issue: 8 Pages: 1316-1326
  • DOI: 10.1210/me.2011-1180
  • Peer Reviewed
• [Journal Article] Pituitary resistance to thyroid hormones : pathophysiology and therapeutic options (2012)
  • Author(s): Suzuki S, Nishio S, Takeda T, Komatsu M
  • Journal Title: Thyroid Res Volume: 5(1) Issue: 1 Pages: 1-1
  • DOI: 10.1186/1756-6614-5-1
  • Peer Reviewed
• [Journal Article] Pituitary resistance to thyroid hormones : pathophysiology and therapeutic options (2011)
  • Author(s): Suzuki S, Shigematsu S, Inaba H, Takei M, Takeda T, Komatsu M
  • Journal Title: Endocrine Volume: 40(3) Issue: 3 Pages: 366-371
  • DOI: 10.1007/s12020-011-9538-2
• [Journal Article] Glucose-incretin interaction revisited [Review] (2011)
  • Author(s): Ishii H, Sato Y, Takei M, Nishio S, Komatsu M
  • Journal Title: Endocrine Journal Volume: 58 Issue: 7 Pages: 519-525
  • DOI: 10.1507/endocrj.EJ11-0064
  • NAID: 10029589169
  • ISSN: 0918-8959, 1348-4540
  • Peer Reviewed
• [Journal Article] Transient polyuria related to central diabetes insipidus caused by lymphocytic infundibulo-neurohypophysitis in a patient treated for Graves' disease. (2010)
  • Author(s): Yamazaki M, Sato A, Nishio S,Ue hara T, Komatsu M.
  • Journal Title: Intern Med. Volume: 49(17) Pages: 1885-1890
  • NAID: 130000336870
  • Peer Reviewed
• [Journal Article] Distinct clinical courses in type l diabetes mellitus induced by peg-interferon-alpha treatment for chronic hepatitis C. (2010)
  • Author(s): Yamazaki M, Sato A, Takeda T,Ko matsu M.
  • Journal Title: Intern Med. Volume: 49(5) Pages: 403-407
  • Peer Reviewed
• [Journal Article] C10sed-loopとopen-loopによる血糖制御 (2010)
  • Author(s): 駒津光久
  • Journal Title: 内分泌等幼猫代謝内科 Volume: 30 Pages: 591-595
• [Journal Article] 機能的膵β細胞量を標的としたあらたな糖尿病治療一GLPI治療の展望一 (2010)
  • Author(s): 駒津光久
  • Journal Title: International Review of Diabetes Volume: 2 Pages: 24-25
• [Presentation] 糖尿病、肥満症の患者にエキセナチドを導入して著効した3例 (2012)
  • Author(s): 西尾真一、石井宏明、武井真大, 山崎雅則、鈴木悟、駒津光久
  • Organizer: 日本糖尿病学会関東甲信越地方会
  • Place of Presentation: 東京
  • Year and Date: 2012-01-21
• [Presentation] The Phytoestrogen Genistein Affects Zebrafish Development through Two Different Pathways (2011)
  • Author(s): Shin-ichi Nishio, Sana Sassi-Messai, Yann Gibert, Hiroaki Ish-i, Masahiro Takei, Satoru Suzuki, Mitsuhisa Komatsu, Patrick Balaguer, Laudet Vincent
  • Organizer: 日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-13
• [Presentation] TRβはPAX6、BETA2を介してインスリン転写活性を増強する (2011)
  • Author(s): 西尾真一、石井宏明、武井真大, 四宮健、宮本高秀、武田貞二、鈴木悟、駒津光久
  • Organizer: 日本甲状腺学会
  • Place of Presentation: 大阪
  • Year and Date: 2011-11-22
• [Presentation] ゼブラフィッシュレプチン受容体OB-Rbは筋形成に必要である (2011)
  • Author(s): 西尾真一、石井宏明、武井真大、駒津光久、Vincent Laudet
  • Organizer: 日本肥満学会
  • Place of Presentation: 淡路
  • Year and Date: 2011-09-23
• [Presentation] 糖尿病、肥満症の患者にエキセナチドを導入して著効した2例 (2011)
  • Author(s): 西尾真一、石井宏明、武井真大, 佐藤亜位、山崎雅則、駒津光久
  • Organizer: 日本肥満学会
  • Place of Presentation: 淡路
  • Year and Date: 2011-09-23
• [Presentation] ERαはPDX-1、BETA2を介してインスリン転写活性を抑制する (2011)
  • Author(s): 西尾真一、石井宏明、武井真大、鈴木悟、駒津光久
  • Organizer: 日本糖尿病学会
  • Place of Presentation: 札幌
  • Year and Date: 2011-05-20
• [Presentation] SHPはPDX-1プロモーター転写活性を抑制する (2011)
  • Author(s): 四宮健、西尾真一、石井宏明、武井真大、柿澤供子、宮本高秀, 鈴木悟、駒津光久
  • Organizer: 日本内分泌学会
  • Place of Presentation: 神戸
  • Year and Date: 2011-04-23
• [Presentation] TRβはPAX6、BETA2を介してインスリン転写活性を増強する (2011)
  • Author(s): 石井宏明、西尾真一、武井真大, 四宮健、宮本高秀、鈴木悟、駒津光久
  • Organizer: 日本内分泌学会
  • Place of Presentation: 神戸
  • Year and Date: 2011-04-22
• [Presentation] ERαはPDX-1、BETA2を介してインスリン転写活性を抑制する (2011)
  • Author(s): 武井真大、西尾真一、四宮健、石井宏明、鈴木悟、駒津光久
  • Organizer: 日本内分泌学会
  • Place of Presentation: 神戸
  • Year and Date: 2011-04-21
• [Presentation] ゼブラフィッシュレプチン受容体OB-Rbは筋形成に必要である (2011)
  • Author(s): 西尾真一、武井真大、石井宏明、鈴木悟、駒津光久、Vincent Laudet
  • Organizer: 日本内分泌学会
  • Place of Presentation: 神戸
  • Year and Date: 2011-04-21
• [Presentation] Leptin receptor homolog in zebrafish controls morphogenetic movements duringgastrulation and is involved in somite formation. (2010)
  • Author(s): 西尾真一,石井宏明,武井真大,鈴木悟,駒津光久、ローデヴァンサン
  • Organizer: 日本分子生物学会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-07
• [Presentation] ゼブラフィッシュ神経系においてCARTの発現はCB1によって調節されている (2010)
  • Author(s): 西尾真一, 石井宏明, 武井真大, 佐藤亜位,山崎雅則
  • Organizer: 日本肥満学会
  • Place of Presentation: 前橋
  • Year and Date: 2010-10-02
• [Presentation] The activation of rat insulin gene II transcription by thyroid hormone receptor beta in pancreatic beta cells.
  • Author(s): Nishio S, Ishi-i H, Suzuki S, Komatsu M
  • Organizer: 日本分子生物学会
  • Place of Presentation: 福岡
• [Presentation] 母子共にDUOX2遺伝子へテロ接合変異、TSB-Ab 陽性で母親が甲状腺機能低下症、児が甲状腺機能低下症および甲状腺腫大を来した一例
  • Author(s): 西尾真一、阿部清美、大平哲史、平林佳奈枝、石井宏明、山崎雅則、櫻井晃洋、鈴木悟、長谷川奉延、駒津光久
  • Organizer: 日本甲状腺学会
  • Place of Presentation: 福岡
• [Presentation] 二相性インスリンアナログ製剤(BIAsp)3回投与法の有効性・安全性に関する研究：50BIAspと70BIAspの比較
  • Author(s): 西尾真一、武田貞二、佐藤亜位、鈴木悟、駒津光久
  • Organizer: 日本糖尿病学会
  • Place of Presentation: 横浜
• [Presentation] The Phytoestrogen Genistein Affects Zebrafish Development through Two Different Pathways.
  • Author(s): Nishio S, Sassi-Messai S, Gibert Y, Ish-I H, Takei M, Suzuki S, Komatsu M, Balaguer P, Laudet V
  • Organizer: 日本内分泌学会
  • Place of Presentation: 名古屋
• [Presentation] プレドニゾロン（PSL）が著効した後天性high density lipoprotein cholesterol (HDL-C)欠損症の1例
  • Author(s): 西尾真一、日高宏哉、有賀雅和、石井宏明、武井真大、山崎雅則、鈴木悟、駒津光久
  • Organizer: 日本内分泌学会
  • Place of Presentation: 名古屋
• [Presentation] ペグミソマントによりコントロール改善した先端巨大症の一例
  • Author(s): 西尾真一、山崎雅則、武田貞二、鈴木悟、駒津光久
  • Organizer: 日本内分泌学会
  • Place of Presentation: 名古屋