Research on the role of βadrenergic receptors in pathogenesis of Meniere's disease

• Project/Area Number: 22591883
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Otorhinolaryngology
• Research Institution: Kagawa University
• Principal Investigator: MORI Nozomu 香川大学, 医学部, 教授 (90124883)
• Co-Investigator(Kenkyū-buntansha): MIYASHITA Takenori 香川大学, 医学部, 助教 (60363214) ; AKIYAMA Kosuke 香川大学, 医学部附属病院, 助教 (40568838) ; INAMOTO Ryuhei 香川大学, 医学部附属病院, その他 (50581047)
• Co-Investigator(Renkei-kenkyūsha): MATSUBARA Ai
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: メニエール病 / β受容体 / 内リンパ嚢 / 内リンパ圧 / ストレス / ラット内リンパ嚢 / 上皮細胞 / 中間部 / アドレナリン受容体 / RT-PCR / 免疫組織学 / β_2-AR / β_3-AR / βアドレナリン受容体 / カテコールアミン / βアゴニスト / 内耳圧

Research Abstract

The results that catecholamines increased endolymphatic hydropressure in the cochlea and semicircular canalin the normal guinea pigs but not in the guinea pigs with obstructed endolymphatic sac (ES) strongly suggest the involvement of ES in increased endolymphatic hydropressure by catecholamines. The findings that catecholamines increased endolymphatic hydropressure in the cochlea to a larger degree than that in the semicircular canalcorrespond to clinical findings that Meniere patients suffer more frequently from the cochlear symptoms than the vestibular symptoms. The strong expression of β2-adrenergic receptor in epithelial cells of the ES intermediate portion accords with the electrophysiological results that catecholamines decrease ES direct current potentialvia β2-action.

Research Products

• [Journal Article] Presence of adrenergic receptors in rat endolymphatic sac epithelial cells (2013)
  • Author(s): Matsubara,A., T. Miyashita, R.Inamoto and N. Mori
  • Journal Title: J. of Membrane Biol Volume: 246 Pages: 109-114
• [Journal Article] Presence of adrenergic receptors in rat endolymphatic sac epithelial cells. (2013)
  • Author(s): Matsubara Ai
  • Journal Title: Journal of Membrane Biology Volume: 246 Issue: 2 Pages: 109-114
  • DOI: 10.1007/s00232-012-9508-5
  • Peer Reviewed
• [Journal Article] The mRNA of claudins is expressed in the endolymphatic sac epithelia. (2012)
  • Author(s): Matsubara,A., T. Miyashita, T. Mori, K. Akiyama, R.Inamoto and N. Mori
  • Journal Title: Auris Nasus Larynx Volume: 39 Pages: 361-364
• [Journal Article] Presence of FXYD6 in the endolymphatic sac epithelia. (2012)
  • Author(s): Miyashita,T., K.Akiyama, R. Inamoto, A. Matsubara, T. Nakagawa, F. Yamaguchi, M. Tokuda and N. Mori
  • Journal Title: Neuroscience Letters Volume: 513 Pages: 47-50
• [Journal Article] Presence of FXYD6 in the endolymphatic sac epithelia (2012)
  • Author(s): Miyashita Takenori
  • Journal Title: Neuroscience Letters Volume: 513 Issue: 1 Pages: 47-50
  • DOI: 10.1016/j.neulet.2012.02.005
  • Peer Reviewed
• [Journal Article] Presence of FXYD6 in the endolymphatic sac epithelia (2012)
  • Author(s): Miyashita, T., Akiyama, K., Mori, N., et al
  • Journal Title: Neurosci Lett Volume: 513 Pages: 47-50
  • Peer Reviewed
• [Journal Article] Evaluation of the vestibular aqueduct in Meniere's disease using multiplanar reconstruction images of CT (2012)
  • Author(s): Miyashita, T., Mori N., et al
  • Journal Title: Auris Nasus Larynx Volume: (on line)(available)
  • Peer Reviewed
• [Journal Article] The mRNAof claudins is expressed in the endolymphatic sac epithelia (2011)
  • Author(s): Matsubara, A., Mori, N., et al
  • Journal Title: Auris Nasus Larynx Volume: (on line)(available)
  • Peer Reviewed
• [Presentation] ラット内リンパ嚢におけるアドレナリンレセプターの発現. (2012)
  • Author(s): 松原あい、宮下武憲、稲本隆平、森 望
  • Organizer: 第30回耳鼻咽喉科ニューロサイエンス研究会
  • Year and Date: 2012-08-25
• [Presentation] イソプロテレノールの内耳への影響. (2012)
  • Author(s): 稲本隆平、宮下武憲、森 望
  • Organizer: 第30回耳鼻咽喉科ニューロサイエンス研究会
  • Year and Date: 2012-08-25
• [Presentation] Presence of adrenergic receptors in the rat endolymphatic sac epithelial cells. (2012)
  • Author(s): Matsubara,A., T. Miyashita, T..Inamoto and N. Mori
  • Organizer: The first Asian Otology Meeting & the 3rd East Asian Symposium on Otology Nagasaki
  • Place of Presentation: Japan
• [Presentation] Role of ion transport in endolymphatic sac in pathogenesis of Meniere'disease (2010)
  • Author(s): Mori N
  • Organizer: Sixth International Symposium on Meniere's Disease and Inner Ear Disorders
  • Place of Presentation: 京都国際会館(京都府)
  • Year and Date: 2010-11-15
• [Presentation] Presence of adrenergic receptors in the rat endolymphatic sac epithelial cells.
  • Author(s): Matsubara Ai
  • Organizer: The first Asian Otology Meeting & the 3rd East Asian Symposium on Otology
  • Place of Presentation: Nagasaki
• [Presentation] ラット内リンパ嚢におけるアドレナリンレセプターの発現．
  • Author(s): 松原 あい
  • Organizer: 第30回耳鼻咽喉科ニューロサイエンス研究会
  • Place of Presentation: 大阪
• [Presentation] イソプロテレノールの内耳への影響．
  • Author(s): 稲本 隆平
  • Organizer: 第30回耳鼻咽喉科ニューロサイエンス研究会
  • Place of Presentation: 大阪
• [Book] Laser Capture Microdissection : Methods and Protocols, Methods in Molecular Biology, Vol.755 (2011)
  • Author(s): Akiyama, K., et al
  • Publisher: Springer Science+Business Media