| Project/Area Number |
22591892
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Kansai University of Welfare Sciences (2011-2013)
Osaka Medical College (2010) |
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Principal Investigator |
MORI Yoshiaki 関西福祉科学大学, 保健医療学部, 教授 (70268192)
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| Co-Investigator(Kenkyū-buntansha) |
MORI Atsuko 大阪医科大学, 医学部, 助教 (40368105)
INUI Takaki 大阪医科大学, 医学部, 助教 (60465614)
YAMAJI Junko 関西福祉科学大学, 健康福祉学部, 准教授 (40340559)
萩森 伸一 大阪医科大学, 医学部, 准教授 (90291799)
窪田 隆裕 大阪医科大学, 医学部, 教授 (10084906)
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| Project Period (FY) |
2010-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | 蝸牛内直流電位 / 辺縁細胞 / 細胞内情報伝達機構 / L型カルシウムチャネル / Cキナーゼ / フォスフォリパーゼC / 細胞内Ca2+濃度 / L型Ca2+チャネル / PKC / PLC / サイクリックヌクレオチド / 細胞内Ca2+ / イオンチャネル / 血管条辺縁細胞 / 細胞内カルシウム / L型Ca^<2+>チャネル / プロティンキナーゼC / アミロリド / ロティンキナーゼC |
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| Research Abstract |
A large positive potential in the endolymph, named the endocochlear potential (EP) has been considered to occur at cochlear stria vascularis. In the previous study, we reported that the decrease in EP induced by transient asphyxia was result from the increase in intracellular calcium ion induced by the activation of L-type calcium channels in the basolateral membrane of the marginal cells. In the present study, we demonstrated that a fall in the EP induced by transient asphyxia was suppressed by the administration of calcium-chelator, L-type calcium channel inhibitor, C kinase (PKC) inhibitors, or phospholipase C (PLC) inhibitors to the endolymph. Thus, asphyxia induced decrease in the EP was induced by the increase in calcium ion in the cells of stria vascularis, through the activation of L-type calcium channels mediated by the PKC activation result from the PLC activation.
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