GLYPICAN 3 targeted therapy in pediatric solid malignant tumors.

• Project/Area Number: 22591980
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Pediatric surgery
• Research Institution: Kyushu University
• Principal Investigator: KINOSHITA Yoahiaki 九州大学, 大学院・医学研究院, 准教授 (80345529)
• Co-Investigator(Kenkyū-buntansha): TAGUCHI Tomoaki 九州大学, 医学研究院, 教授 (20197247) ; TAJIRI Tatsuro 京都府立医科大学, 医学研究科, 教授 (80304806) ; SUZAKI Ryota 九州大学, 大学病院, 助教 (10403990) ; TANAKA Sakura 九州大学, 医学研究院, 共同研究員 (40467923)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: GLYPICAN3 / 小児固形悪性腫瘍 / 腫瘍マーカー / AFP / 分子標的治療

Research Abstract

【Purpose】The purpose of this study is to analyze the usefulness of Glypican3 as a novel diagnostic tumor marker for pediatric solid malignant tumor, and to establish the new diagnostic system. Final goal is to develop the immuno-genetic therapy targeted for the Glypican3.【Method】The immunohistochemical analysis and serological analysis of Glypican3 and AFP was performed for the pathological materials and patient’s serum. 【Results】Immunohistochemlically and serologically, most cases of hepatoblastoma and malignant germ cell tumor showed positive for Glypican3, and one of thirds Wilms tumor, alveolar type rhabdomyosarcoma and undifferentiated sarcoma were also positive. Concerning AFP, most cases of hepatoblastoma and malignant germ cell tumor showed positive. Concerning non-neoplastic patients’, serum Glypican 3 was high just after birth and gradually decreased according to their age. The level normalized by the 1 year.【Discussion】Glypican3 was confirmed as the novel diagnostic marker for pediatric solid malignant tumors. Based on this research, for the refractory cases of pediatric solid malignant tumor over 1 year, such new treatment immuno-genetic therapy targeted forGlypican 3 will be expected.

Research Products

• [Journal Article] Glypican 3 expression in tumors with loss of SMARCB1/INI1 protein expression. (2013)
  • Author(s): Kohashi K
  • Journal Title: Human Pathology Volume: 44 Issue: 4 Pages: 526-533
  • DOI: 10.1016/j.humpath.2012.06.014
  • Peer Reviewed
• [Journal Article] The prognostic significance of blastemal predominant histology in initially resected Wilms'tumors: a report from the Study Group for Pediatric Solid Tumors in the Kyushu Area, Japan (2012)
  • Author(s): Kinoshita Y, Suminoe A, Inada H, Yagi M, Yanai F, Zaizen Y, Nishi M, Inomata Y, Kawakami K, Matsufuji H, Suenobu S, Handa N, Kohashi K, Oda Y, Hara T, Taguchi T
  • Journal Title: J Pediatr Surg Volume: 47 Issue: 12 Pages: 2205-2209
  • DOI: 10.1016/j.jpedsurg.2012.09.009
  • Peer Reviewed
• [Journal Article] Implications of surgical intervention in the treatment of neuroblastomas: 20-year experience of a single institution (2012)
  • Author(s): Tajiri T, Souzaki R, Kinoshita Y, Koga Y, Suminoe A, Hara T, Taguchi T
  • Journal Title: Surg Today Volume: 42 Issue: 3 Pages: 220-224
  • DOI: 10.1007/s00595-011-0053-0
  • NAID: 10030231044
  • Peer Reviewed
• [Journal Article] Surgical intervention strategies for pediatric ovarian tumors : experience with 60 cases at one institution (2012)
  • Author(s): Tajiri T, Souzaki R, Kinoshita Y, Yosue R, Kohashi K, et al
  • Journal Title: Pediatr Surg Int Volume: 28 Issue: 1 Pages: 27-31
  • DOI: 10.1007/s00383-011-3004-3
  • Peer Reviewed
• [Journal Article] Surgical strategies for unresectable hepatoblastomas. (2012)
  • Author(s): Tajiri T, Hiyama E, et al
  • Journal Title: Journal of Pediatric Surgery. Volume: 47 Issue: 12 Pages: 2194-2198
  • DOI: 10.1016/j.jpedsurg.2012.09.006
  • Peer Reviewed
• [Journal Article] The prognostic significance of blastemal predominant histology in initially resected Wilms tumors: a report from the Study Group for Pediatric Solid Tumors in the Kyushu Area, Japan (2012)
  • Author(s): Kinoshita Y, Suminoe A, Inada A, et al
  • Journal Title: J Pediatr Surg Volume: 47 Pages: 2205-2209
  • Peer Reviewed
• [Journal Article] Glypican 3 expression in tumors with loss of SMARCB1/INI1 protein expression (2012)
  • Author(s): Kohashi K, Kinoshita Y, Taguchi T, et al
  • Journal Title: Human Pathol Volume: 44 Pages: 526-533
  • Peer Reviewed
• [Journal Article] Implications of surgical intervention in the treatment of neuroblastoma : 20-year experience of a single institution (2012)
  • Author(s): Tajiri T, Souzaki R, Kinoshita Y, et al
  • Journal Title: Surg Today Volume: 42 Pages: 220-224
  • NAID: 10030231044
  • Peer Reviewed
• [Journal Article] Successful resection of an undifferentiated sarcoma in a child using a real-time surgical navigation system in an open magnetic resonance imaging operation room (2011)
  • Author(s): Souzaki R, Kinoshita Y, Matsuura T, Tajiri T, Taguchi T, Ieiri S, Hong J, Uemura M, Konishi K, Tomikawa M, Tanoue K, Hashizume M, Koga Y, Suminoe A, Hara T, Kohashi K, et al
  • Journal Title: J Pediatr Surg Volume: 46 Issue: 3 Pages: 608-611
  • DOI: 10.1016/j.jpedsurg.2010.11.043
  • Peer Reviewed
• [Journal Article] Correlation between the number of segmental chromosome aberrations and the age at diagnosis of diploid neuroblastomas without MYCN amplification (2011)
  • Author(s): Souzaki R, Tajiri T, Teshiba R, Kinoshita Y, Yosue R, Kohashi K, et al
  • Journal Title: J Pediatr Surg Volume: 46 Issue: 12 Pages: 2228-2232
  • DOI: 10.1016/j.jpedsurg.2011.09.005
  • Peer Reviewed
• [Journal Article] Successful resection of an undifferentiated sarcoma in a child using areal-time surgical navigation system in an open magnetic resonance imaging operation room (2011)
  • Author(s): Souzaki R, Kinoshita Y, et al
  • Journal Title: J Pediatr Surg Volume: 46 Pages: 608-611
  • Peer Reviewed
• [Journal Article] 肝移植のタイミングに難渋した巨大肝芽腫の1例 (2010)
  • Author(s): 高橋由紀子, 西本祐子, 松浦俊治, 林田真, 田尻達郎, 増本幸二, 藤田桂子, 木下義晶, 宗崎良太, 武冨紹信, 前原喜彦, 猪股裕紀洋, 田口智章
  • Journal Title: 臨床と研究 Volume: 87 Pages: 1288-1289
  • Peer Reviewed
• [Journal Article] 樹状細胞療法を施行した小児腎細胞癌の1例 (2010)
  • Author(s): 竜田恭介, 木下義晶, 田尻達郎, 中辻隆徳, 東真弓, 宗崎良太, 江藤正俊, 立神勝則, 孝橋賢一, 内藤誠二, 恒吉正澄, 田口智章
  • Journal Title: 小児がん Volume: 47 Pages: 441-446
  • NAID: 110008661027
  • URL: http://ci.nii.ac.jp/els/110008661027.pdf?id=ART0009741000&type=pdf&lang=jp&host=cinii&order_no=&ppv_type=0&lang_sw=&no=1369212538&cp=
  • Peer Reviewed
• [Journal Article] Concordance for neuroblastoma in monozygotic twins: case report and review of the literature (2010)
  • Author(s): Tajiri T, Souzaki R, Kinoshita Y, Tanaka S, Koga Y, Suminoe A, Hara T, Kohashi K, Oda Y, Masumoto K, Ohira M, Nakagawara A, Taguchi T
  • Journal Title: J Pediatr Surg Volume: 45 Issue: 12 Pages: 2312-2316
  • DOI: 10.1016/j.jpedsurg.2010.08.025
  • Peer Reviewed
• [Journal Article] Successful treatment of advanced pancreatoblastoma by a pylorus-preserving pacreatoduodenectomy after radiation and high-dose chemotherapy (2010)
  • Author(s): Souzaki R, Tajiri T, Kinoshita Y, Tanaka S, Koga Y, Suminoe A, Hara T, Kohashi K, Oda Y, Taguchi T
  • Journal Title: Pediatr Surg Int Volume: 26 Issue: 10 Pages: 1045-1048
  • DOI: 10.1007/s00383-010-2655-9
  • Peer Reviewed
• [Journal Article] Hedgehog signaling pathway in neuroblastoma differentiation (2010)
  • Author(s): Souzaki R, Tajiri T, Souzaki M, Kinoshita Y, Tanaka S, Kohashi K, Oda Y, Katano M, Taguchi T
  • Journal Title: J Pediatr Surg Volume: 45 Issue: 12 Pages: 2299-2304
  • DOI: 10.1016/j.jpedsurg.2010.08.020
  • Peer Reviewed
• [Journal Article] 新規腫瘍マーカーによる小児固形悪性腫瘍における診断システムの確立 (2010)
  • Author(s): 木下義晶
  • Journal Title: 第5回ふくおか「臨床医学研究賞」 Pages: 12-13
• [Presentation] 小児腎腫瘍に対する術前化学療法の検討 (2012)
  • Author(s): 木下義晶、宗崎良太、代居良太、大場詩子、古賀友紀、住江愛子、三好きな、孝橋賢一、原寿郎、小田義直、田口智章
  • Organizer: 第55回日本小児血液・がん学会
  • Place of Presentation: 横浜
• [Presentation] 小児腎腫瘍の新病理分類に基づく解析 (2011)
  • Author(s): 木下義晶
  • Organizer: 第53回日本小児血液・がん学会
  • Place of Presentation: ベイシア文化ホール(群馬)
  • Year and Date: 2011-11-26
• [Presentation] 当科における巨大仙尾部奇形腫の検討と胎児治療に関する展望 (2011)
  • Author(s): 木下義晶
  • Organizer: 第48回日本小児外科学会
  • Place of Presentation: ITF東京ファッションタウンビル(東京)
  • Year and Date: 2011-07-20
• [Presentation] Clinical significance of serum Glypican 3 level as the novel tumor marker in pediatric malignant solid tumors (2010)
  • Author(s): Kinoshita Y
  • Organizer: SIOP 2010
  • Place of Presentation: John B.Hynes Veterans Memorial Convention Center (USA)
  • Year and Date: 2010-10-23
• [Presentation] Clinical implications of serum Glypican 3 level as the novel tumor marker in pediatric malignant solid tumors (2010)
  • Author(s): Kinoshita Y
  • Organizer: XXIII Internatinal Symposium of Pediatric Surgical Research
  • Place of Presentation: 東京ガーデンパレス(東京都)
  • Year and Date: 2010-09-14
• [Presentation] 小児固形悪性腫瘍における新規腫瘍マーカーとしてのGlypican3の有用性 (2010)
  • Author(s): 木下義晶
  • Organizer: 第47回日本小児外科学会学術集会
  • Place of Presentation: 愛知県産業労働センター(愛知県)
  • Year and Date: 2010-06-18
• [Presentation] Clinical implications of serum Glypican 3 level as the novel tumor marker in pediatric malignant solid tumors (2010)
  • Author(s): Kinoshita Y
  • Organizer: The 43^<rd> Annual Meeting of Pacific Association of Pediatric Surgeons
  • Place of Presentation: 神戸ポートピアホテル(兵庫県)
  • Year and Date: 2010-05-26
• [Presentation] New strategy is necessary for blastemal predominant type of Wilms tumor: a report from the Study Group for Pediatric Solid Tumors in the Kyushu Area Japan
  • Author(s): Kinoshita Y, Suminoe A, Taguchi T, et al
  • Organizer: 45th Pacific Association of Pediatric Surgeons
  • Place of Presentation: 上海(中国)