Relationship of ECM superstructure and cytokine in the osteogenesis

• Project/Area Number: 22592038
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Morphological basic dentistry
• Research Institution: Okayama University
• Principal Investigator: Kawai Mariko 岡山大学, 医歯(薬)学総合研究科, 助教 (40379839)
• Co-Investigator(Kenkyū-buntansha): 十川 紀夫 岡山大学, 医歯(薬)学総合研究科, 准教授 (30236153)
• Research Collaborator: Neubueser Annette Freiburug University, Dr. Prof. ; herrmann David Freiburug University, Dr.
• Project Period (FY): 2010-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 発生・分化 / 細胞外基質 / サイトカイン / 再生医学 / 発生・文化 / 骨 / 再生 / 発生

Outline of Final Research Achievements

The superstructure of extracelluar matrix controls cytokine's activities. In this study, we tried to analyze how laminin super structure effects bone formation through the interaction fgf signaling. We made monoclonal antibodies for laminin super structure and applied them to in vitro and in vivo bone formation models. Our results might conclude that laminin super structure could control bone formation and calcification.

Research Products

• [Journal Article] BMP-2 gene transfer under various conditions with in vivo electroporation and bone induction (2012)
  • Author(s): Hiromitsu Yamamoto, Mariko Kawai, 他8名
  • Journal Title: Asian Journal of Oral and Maxillofacial Surgery Volume: 24 Issue: 1 Pages: 49-53
  • DOI: 10.1016/j.ajoms.2011.10.006
  • Peer Reviewed
• [Presentation] fgf conditional knock out mice derived chondrogenesis andosteogenesis in the cranial bone formation (2013)
  • Author(s): 河井まりこ、山本敏男
  • Organizer: 第55回歯科基礎医学会総会•学術集会
  • Place of Presentation: 岡山
• [Presentation] Simple strategy for bone regeneration with a BMP-2/7 gene expression cassette vector (2010)
  • Author(s): 河井 まりこ
  • Organizer: 日本骨代謝学会
  • Place of Presentation: 京王プラザホテル(東京)
  • Year and Date: 2010-07-23