Clarify of extra-territorial pain hyperalgesia in maxillofacial region following a trigeminal nerve injury.

• Project/Area Number: 22592039
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Morphological basic dentistry
• Research Institution: Hiroshima University
• Principal Investigator: WATANABE Mineo 広島大学, 医歯薬保健学研究院, 助教 (80325183)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 口腔解剖学(含組織学・発生学) / 病態痛 / 口腔解剖学（含組織学・発生学） / 異所性疼痛 / 三叉神経 / ギャップ結合 / ラット / 核酸分子

Research Abstract

Intravenous injection of bone marrow stromal cells reversed pain hypersensitivity in rats after the infraorbital nerveinjury. The pain hypersensitivity was rekindled by naloxone hydrochloride, an opioid receptor antagonist（Guo, Watanabe et al. Stem cells 2011）. The infraorbital nerve injury or the mental nerve transection induced pain hyperalgesia, and up-regulation of P2X7receptor, TNF-αand phosphorylated (p)-p38 MAPK in the trigeminal sensory nuclear complex. Pain hyperalgesia or up-regulation of TNF-αin was inhibited by a P2X7receptor antagonist or p-p38 MAPK inhibitor（Ito, Watanabe et al. European J Pain 2013, Murasaki, Watanabe et al. J Dent Res 2013）.

Research Products

• [Journal Article] Tanne K.P2X7receptor in the trigeminal sensory nuclear complex contributes to tactile allodynia/hyperalgesia following trigeminal nerve injury. (2013)
  • Author(s): Ito G, Suekawa Y, Watanabe M, Takahashi K, Inubushi T, Murasaki K, Hirose N, Hiyama S, Uchida T
  • Journal Title: Eur J Pain Pages: 85-99
  • Peer Reviewed
• [Journal Article] Tanne K.P2X7receptor and cytokines contribute to extra-territorial facial pain (2013)
  • Author(s): Murasaki K, Watanabe M, Takahashi K, Ito G, Suekawa Y, Inubushi T, Hirose N, Uchida T
  • Journal Title: J Dent Res Pages: 260-5
  • Peer Reviewed
• [Journal Article] P2X7 receptor in the trigeminal sensory nuclear complex contributes to tactile allodynia/hyperalgesia following trigeminal nerve injury. (2013)
  • Author(s): Ito G, Suekawa Y, Watanabe M.
  • Journal Title: Eur J Pain Volume: 17 Issue: 2 Pages: 185-199
  • DOI: 10.1002/j.1532-2149.2012.00174.x
  • Peer Reviewed
• [Journal Article] P2X7 receptor and cytokines contribute to extra-territorial facial pain. (2013)
  • Author(s): Murasaki K, Watanabe M.
  • Journal Title: J Dent Res Volume: 92 Issue: 3 Pages: 260-265
  • DOI: 10.1177/0022034512474668
  • Peer Reviewed
• [Journal Article] Bone marrow stromal cells produce long-term pain relief in rat models of persistent pain (2011)
  • Author(s): Guo W, Wang H, Zou S, Gu M, Watanabe M, Wei F, Dubner R, Huang GT, Ren K
  • Journal Title: Stem Cells Pages: 1294-303
  • Peer Reviewed
• [Journal Article] IL-1beta in the trigeminal subnucleus caudal is contributes to extra-territorial allodynia/hyperalgesia following a trigeminal nerve injury (2011)
  • Author(s): Takahashi K, Watanabe M, Suekawa Y, Ito G, Inubushi T, Hirose N, Murasaki K, Hiyama S, Uchida T, Tanne K
  • Journal Title: European Journal of Pain Volume: 15 Issue: 5
  • DOI: 10.1016/j.ejpain.2010.10.006
  • Peer Reviewed
• [Journal Article] Bone marrow stromal cells produce long-term pain relief in rat models of persistent pain (2011)
  • Author(s): W.Guo, H.Wang, S.Zou, M.Gu, M.Watanabe, F.Wei, R.Dubner, G.Huang, K.Ren
  • Journal Title: Stem Cells Volume: (in press)
  • Peer Reviewed
• [Presentation] 顎顔面領域の神経因性疼痛発現におけるP2X7受容体の役割. (2013)
  • Author(s): 渡邉峰朗, 伊藤剛志, 村崎恭子, 高橋拡史, 末川洋平, 広瀬尚人, 丹根一夫, 内田 隆
  • Organizer: 第118回日本解剖学会総会・全国学術集会
  • Place of Presentation: 高松市
• [Presentation] 顎顔面領域の神経因性疼痛発現におけるP2X7受容体の役割 (2013)
  • Author(s): 渡邉峰朗
  • Organizer: 第118回日本解剖学会総会・全国学術集会
  • Place of Presentation: 高松市
• [Presentation] 顎顔面領域の神経因性疼痛発現における P2X7受容体の役割. 第96回広島大学歯学会(併催) (2012)
  • Author(s): 渡邉峰朗, 伊藤剛志, 村崎恭子, 高橋拡史, 末川洋平, 広瀬尚人, 丹根一夫,内田 隆
  • Organizer: 第51回広島県歯科医学会
  • Place of Presentation: 広島市
  • Year and Date: 2012-10-28
• [Presentation] IL-1βin trigeminal nucleus caudalis contributes to extra-territorial allodynia/hyperalgesia following a trigeminal nerve injury (2012)
  • Author(s): Watanabe M, Hiyama S, Uchida T
  • Organizer: 第117回 日本解剖学会総会・全国学術集会
  • Place of Presentation: 甲府市
• [Presentation] Aletred glutamate homeostasis in descending circuitry correlates with the emergence of pain chronicity. (2012)
  • Author(s): Guo W, Gu M, Zou S, Watanabe M, Wei F, Dubner R, Ren K
  • Organizer: 14th World Congress on Pain
  • Place of Presentation: Milano, Italy
• [Presentation] A role of P2X7receptor in neuropathic pain following trigeminal nerve injury. (2012)
  • Author(s): Watanabe M, Uchida T.
  • Organizer: 第54回歯科基礎医学会学術大会・総会
  • Place of Presentation: 郡山市
• [Presentation] 三叉神経傷害後の顔面領域における異所性疼痛発現に対する小膠細胞の役割 (2012)
  • Author(s): 村崎恭子, 渡邉峰朗, 伊藤剛志, 高橋拡史, 内田 隆, 丹根一夫
  • Organizer: 第71回 日本矯正歯科学会大会
  • Place of Presentation: 日盛岡市
• [Presentation] TNF-αin the trigeminal subnucleus caudalis contributes to extra-territorial allodynia/hyperalgesia following a trigeminal nerve injury. (2012)
  • Author(s): Murasaki K, Watanabe M, Takahashi K, Ito G, Suekawa Y, Inubushi T, Hirose N, Uchida T, Tanne K.
  • Organizer: The 45th Scientific Congress of Korean Association of Orthodontists 4th Joint Symposium of KAO and JOS
  • Place of Presentation: Seoul, Korea
• [Presentation] Altered glutamate homeostasis in descending circuitry correlates with the emergence of pain chronicity (2012)
  • Author(s): Mineo Watanabe
  • Organizer: 14th World Congress on Pain
  • Place of Presentation: Milano, Italy
• [Presentation] TNF-α in the trigeminal subnucleus caudalis contributes to extra-territorial allodynia/hyperalgesia following a trigeminal nerve injury (2012)
  • Author(s): Kyoko Murasaki
  • Organizer: The 45th Annual Scientific Congress of Korean Association of Orthodontists The 4th Joint Symposium of KAO and JOS Nov 1-3, 2012, COEX, Seoul, Korea
  • Place of Presentation: Seoul, Korea
• [Presentation] A role of P2X7 receptor in neuropathic pain following trigeminal nerve injury (2012)
  • Author(s): Mineo Watanabe
  • Organizer: 第54回歯科基礎医学会学術大会・総会
  • Place of Presentation: 郡山市
• [Presentation] 三叉神経傷害後の顔面領域における異所性疼痛発現に対する小膠細胞の役割 (2012)
  • Author(s): 村崎恭子
  • Organizer: 第 71回 日本矯正歯科学会大会
  • Place of Presentation: 盛岡市
• [Presentation] IL-1β in trigeminal nucleus caudalis contributes to extra-territorial allodynia/hyperalgesia following a trigeminal nerve injury (2012)
  • Author(s): Mineo Watanabe, Shinji Hiyama, Takashi Uchida
  • Organizer: 第117回日本解剖学会総会・全国学術集会
  • Place of Presentation: 甲府市
• [Presentation] Bone marrow stromal cells produce longterm pain relief in rat models of persistent orofacial pain (2011)
  • Author(s): Guo W, Zou S, Gu M, Wei F, Watanabe M, Huang G, Dubner R, Ren K
  • Organizer: 41st annual meeting of Society for Neuroscience
  • Place of Presentation: Washington DC, U.S.A
• [Presentation] Bone marrow stromal cells produce longterm pain relief in rat models of persistent orofacial pain (2011)
  • Author(s): W.GUO, S.ZOU, M.GU, F.WEI, M.WATANABE, G.T.J.HUANG, R.DUBNER, K.REN
  • Organizer: 41st annual meeting of Society for Neuroscience
  • Place of Presentation: Washington DC・U.S.A
• [Presentation] Astrocytic Interleukin-1βcontributes to neuropathic pain following mental nerve injury. (2010)
  • Author(s): Takahashi K, Watanabe M, Suekawa Y, Ito G, Murasaki K, Hirose N, Hiyama S, Uchida T, Tanne K.
  • Organizer: 88th General Session & Exhibition of the IADR
  • Place of Presentation: Barcelona, Spain