| Project/Area Number |
22592070
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional basic dentistry
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| Research Institution | Nagasaki University |
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Principal Investigator |
NEMOTO Yuko 長崎大学, 医歯薬学総合研究科, 准教授 (10164667)
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| Co-Investigator(Kenkyū-buntansha) |
NEMOTO Takayuki 長崎大学, 大学院・医歯薬学総合研究科, 教授 (90164665)
KIMURA Shigenobu 岩手医科大学, 歯学部, 教授 (10177917)
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| Co-Investigator(Renkei-kenkyūsha) |
BABA Tomomi 長崎大学, 大学院・医歯薬学総合研究科, 助教 (60189727)
KOBAYAKAWA Takeshi 長崎大学, 大学院・医歯薬学総合研究科, 教務職員 (10153587)
ONO Toshio 長崎大学, 大学院・医歯薬学総合研究科, 研究員 (80050607)
SHIMOYAMA Yu 岩手医科大学, 歯学部, 助教 (90453331)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | セリンプロテアーゼ / プロテアーゼ / リコンビナントタンパク質 / 病原因子 / 黄色ブドウ球菌 / 活性化 / ジペプチジルペプチダーゼ / ブドウ球菌 / 酵素 |
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| Research Abstract |
Shortening of the propeptide of Glu-specific serine peptidase(GluV8) family commonly occurred in an auto-catalytic manner. Accompanied with propeptide-shortening, the pro-mature junction became more susceptible toward the maturation enzymes. We demonstrated the in vitro Glu-specific endopeptidase activity of exfoliative toxin (ET) A for the first time, which was measured with Escherichia coli-expressed recombinant ETAs and synthetic peptidyl substrates. The animal model revealed that the proteolytic activity was indispensable for exfoliation of the skin. In addition, we determined characteristics of DPP11 and DPP7, and proposed a novel phylogenetic system of these family molecules.
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