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Analysis of a crosstalk signaling between mechanical stress and lyso-phospholipid signals in periodontal ligament

Research Project

Project/Area Number 22592074
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Functional basic dentistry
Research InstitutionHealth Sciences University of Hokkaido

Principal Investigator

ARAKAWA Toshiya  北海道医療大学, 歯学研究科, 講師 (40306254)

Co-Investigator(Kenkyū-buntansha) TAKUMA Taishin  北海道医療大学, 歯学部, 教授 (40095336)
SHITARA Akiko  北海道医療大学, 歯学部, 助教 (30508718)
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords歯学 / ストレス / 脂質 / シグナル伝達 / 歯根膜 / メカニカルストレス / リゾホスファチジン酸 / 歯の移動 / 歯根吸収 / 骨関連遺伝子 / ヒト歯根膜 / DNAチップ
Research Abstract

LPA1 and LPA6 of LPA receptors were highly expressed; autotaxin (lyso-PLD) was also highly expressed, however, PA-PLA_ and PS-PLA_1 were not expressed. MS stimulated autotaxin mRNA in human PDL cells. ID1 (Inhibitor of DNA binding 1), EGR1 (Early growth response 1), SGK1 (Regulator of G-protein signaling 2) and DUSP1 (Dual specificity phosphatase 1) were upregulated in PDL cells. LPA produced from PDL cells was analyzed by Thin-Layer Chromatography using ^C-labeled palmitic acid and lysophosphatidylcholine. An enzyme for LPA synthesis, lyso-Phospholipase D (lyso-PLD) was induced by MS after 6h. ERK phosphorylation was determined by MS or LPA treatment at 10 min. LPA was synthesized by PDL cell with MS.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (33 results)

All 2013 2012 2011 2010 Other

All Journal Article (8 results) (of which Peer Reviewed: 4 results) Presentation (25 results)

  • [Journal Article] Gravity loading induces adenosine triphosphate release and phosphorylation of extracellular signal-regulated kinases inhuman periodontal ligament cells2013

    • Author(s)
      Ito Mai, Arakawa Toshiya, Okayama Miki, Shitara Akiko, Mizoguchi Itaru, Takuma Taishin
    • Journal Title

      Journal of Investigative and Clinical Dentistry

      Volume: 4(in press)

    • Related Report
      2012 Final Research Report
  • [Journal Article] Isoproterenol stimulates transient SNAP23-VAMP2 interaction in rat parotid glands2013

    • Author(s)
      Taishin Takuma, Akiko Shitara, Toshiya Arakawa, Miki Okayama, Itaru Mizoguchi, Yoshifumi Tajima
    • Journal Title

      FEBS Letters

      Volume: 587 Pages: 583-589

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Journal Article] Gravity loading induces adenosine triphosphate release and phosphorylation of extracellular signal-regulated kinases inhuman periodontal ligament cells2013

    • Author(s)
      Mai Ito, Toshiya Arakawa, Miki Okayama, Akiko Shitara, Itaru Mizoguchi, Taishin Takuma
    • Journal Title

      Journal of Investigative and Clinical Dentistry

      Volume: 4 Issue: 4 Pages: 1-9

    • DOI

      10.1111/jicd.12049

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] VAMP4 is required to maintain the ribbon structure of the Golgi apparatus2013

    • Author(s)
      Shitara A, Shibui T, Okayama M, Arakawa T, Mizoguchi I, Shakakura Y, Takuma T
    • Journal Title

      Mol Cell Biochem

      Volume: 378

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] SNARE proteins are not excessive for the formation of post-Golgi SNARE complexes in HeLa cells2012

    • Author(s)
      Miki Okayama, Akiko Shitara, Toshiya Arakawa Yoshifumi, Tajima, Itaru Mizoguchi, Taishin Takuma
    • Journal Title

      Molecular and Cellular Biochemistry

    • Related Report
      2012 Final Research Report
  • [Journal Article] SNARE proteins are not excessive for the formation of post-Golgi SNARE complexes in HeLa cells2012

    • Author(s)
      Okayama M, Shitara A, Arakawa Y, Takuma T, Tajima Y, Mizoguchi I.
    • Journal Title

      Mol Cell Biochem(on line)

      Volume: 366 Issue: 1-2 Pages: 159-168

    • DOI

      10.1007/s11010-012-1293-z

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A PCR-based method for constructing a linear vector with site-specific DNA methylation2011

    • Author(s)
      Toshiya Arakawa, Toru Ohta, Yoshihiro Abiko, Miki Okayama, Itaru Mizoguchi and Taishin Takuma
    • Journal Title

      Analytical Biochemistry

      Volume: 416 Pages: 211-217

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
  • [Journal Article] Nicotine induces upregulated expression of beta defensin-2 via the P38MAPK pathway in the HaCaT human keratinocyte cell line2011

    • Author(s)
      Nakamura S, Saitoh M, Yamazaki M, Nishimura M, Kurashige Y, Arakawa T, Takuma T, Kaku T, Abiko Y
    • Journal Title

      Med Mol Morphol

      Volume: 43(4) Pages: 204-10

    • NAID

      10027752317

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] Crosstalk Between Lysophosphatidic Acid and Mechanical Stress in Periodontal Ligament2013

    • Author(s)
      Toshiya Arakawa, Miki Okayama, Akiko Shitara, Itaru Mizoguchi, Taishin Takuma
    • Organizer
      IADR/AADR/CADR 91th General Session and Exhibition
    • Place of Presentation
      Seattle convention center
    • Year and Date
      2013-03-21
    • Related Report
      2012 Final Research Report
  • [Presentation] Analysis of lysophosphatidic acid signaling in periodontal Ligament2012

    • Author(s)
      Toshiya Arakawa, Miki Okayama, Nobuko Obara, Akiko Shitara, Itaru Mizoguchi,Taishin Takuma
    • Organizer
      41st Annual Meeting & Exhibition of the AADR
    • Place of Presentation
      Tampa convention center(Florida USA)
    • Year and Date
      2012-03-24
    • Related Report
      2012 Final Research Report
  • [Presentation] Analysis of lysophosphatidic acid signaling in periodontal Ligament2012

    • Author(s)
      Toshiya Arakawa, Miki Okayama, Nobuko Obara, Akiko Shitara, Itaru Mizoguchi, Taishin Takuma
    • Organizer
      41st Annual Meeting & Exhibition of the AADR
    • Place of Presentation
      タンパ コンベンションセンター(Florida USA)(poster presentation)
    • Year and Date
      2012-03-24
    • Related Report
      2011 Annual Research Report
  • [Presentation] ヒト歯根膜におけるリゾホスファチジン酸の合成およびシグナル経路の解析2012

    • Author(s)
      荒川俊哉, 岡山三紀, 設楽彰子, 溝口 到, 田隈泰信
    • Organizer
      第85回日本生化学会
    • Place of Presentation
      福岡市
    • Related Report
      2012 Final Research Report
  • [Presentation] ヒト歯根膜細胞におけるリゾホスファチジン酸シグナルの解析2012

    • Author(s)
      岡山三紀、荒川俊哉、池 田和貴、田隈泰信、溝口到
    • Organizer
      第71回日本矯正歯科学会学術大会
    • Place of Presentation
      岩手県 盛岡市
    • Related Report
      2012 Final Research Report
  • [Presentation] ヒト歯根膜におけるリゾホスファチジン酸シグナル2012

    • Author(s)
      荒川俊哉、岡山三紀、小原伸子、設楽彰子、入江一元、溝口到、田隈泰信
    • Organizer
      第54回歯科基礎医学会学術大会
    • Place of Presentation
      福島県 郡山市
    • Related Report
      2012 Final Research Report
  • [Presentation] 特異的メチル基導入ベクターの ES 細胞での発現調節2012

    • Author(s)
      荒川 俊哉、安彦 善裕、岡山 三紀、溝口到、設楽 彰子、田隈 泰信
    • Organizer
      第52回歯科基礎医学会学術大会
    • Place of Presentation
      東京都 船堀
    • Related Report
      2012 Final Research Report
  • [Presentation] VAMP4 ノックダウン細胞におけるゴルジ体の断片化と初期エンドソームの分散化2012

    • Author(s)
      設楽 彰子、岡山 三紀、荒川 俊哉、溝口到、田隈 泰信
    • Organizer
      第52回歯科基礎医学会学術大会
    • Place of Presentation
      東京都 船堀
    • Related Report
      2012 Final Research Report
  • [Presentation] ヒト歯根膜細胞のメカニカルストレスによる遺伝子発現2011

    • Author(s)
      荒川俊哉、岡山三紀、溝口到、設楽彰子、田隈泰信
    • Organizer
      第53回歯科基礎医学会学術大会
    • Place of Presentation
      岐阜県長良川国際会議場(口演発表)
    • Year and Date
      2011-10-02
    • Related Report
      2011 Annual Research Report
  • [Presentation] ヒト歯根膜におけるリゾホスファチジン酸シグナル解析2011

    • Author(s)
      荒川俊哉、岡山三紀、小原伸子、設楽彰子、柴田俊一、溝口到、田隈泰信
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      国立京都国際会館(ポスター発表)
    • Year and Date
      2011-09-22
    • Related Report
      2011 Annual Research Report
  • [Presentation] ヒト歯根膜におけるリゾホスファチジン酸シグナルの解析2011

    • Author(s)
      荒川俊哉、岡山三紀、小原伸子、設楽彰子、柴田俊一、溝口到、田隈泰信
    • Organizer
      第84回日本生化学会
    • Place of Presentation
      京都国際会議場
    • Related Report
      2012 Final Research Report
  • [Presentation] メカニカルストレスに誘導されるヒト歯根膜細胞の遺伝子検索2011

    • Author(s)
      岡山三紀、荒川俊哉、池田和貴、田隈泰信、溝口 到
    • Organizer
      第70回日本矯正歯科学会学術大会
    • Place of Presentation
      名古屋国際会議場
    • Related Report
      2012 Final Research Report
  • [Presentation] ゴルジ体のリボン構造形におけるVAMP4の重要性2011

    • Author(s)
      設楽彰子、渋井徹、岡山三紀、荒川俊哉、溝口 到、坂倉康則、田隈泰信
    • Organizer
      第53回歯科基礎医学会学術大会
    • Place of Presentation
      岐阜県 長良川国際会議場
    • Related Report
      2012 Final Research Report
  • [Presentation] ヒト歯根膜細胞のメカニカルストレスによる遺伝子発現2011

    • Author(s)
      荒川俊哉、岡山三紀、溝口到、設楽彰子、田隈泰信
    • Place of Presentation
      岐阜県 長良川国際会議場
    • Related Report
      2012 Final Research Report
  • [Presentation] ラット耳下腺調節開口分泌に関わるSNARE複合2011

    • Author(s)
      田隈泰信、設楽彰子、岡山三紀、荒川俊哉、溝口到
    • Organizer
      第53回歯科基礎医学会学術大会
    • Place of Presentation
      岐阜県 長良川国際会議場
    • Related Report
      2012 Final Research Report
  • [Presentation] Gene expression by mechanical stress in human periodontal ligament cells2011

    • Author(s)
      Toshiya ARAKAWA, Miki OKAYAMA, Yoshihiro ABIKO, Itaru MIZOGUCHI, AkikoSHITARA, and Taishin TAKUMA
    • Organizer
      IADR/AADR/CADR 89th General Session and Exhibition
    • Place of Presentation
      San Diego,CA USA
    • Related Report
      2012 Final Research Report
  • [Presentation] メカニカルストレスに誘導されるヒト歯根膜細胞の遺伝子検索2011

    • Author(s)
      岡山三紀、荒川俊哉、池田和貴、田隈泰信、溝口到
    • Organizer
      第70回日本矯正歯科学会学術大会
    • Place of Presentation
      名古屋国際会議場(ポスター発表)
    • Related Report
      2011 Annual Research Report
  • [Presentation] Gene expression by mechanical stress in human periodontal ligament cells2011

    • Author(s)
      T.ARAKAWA, M.OKAYAMA, Y.ABIKO, I.MIZOGUCHI, A.SHITARA, T.TAKUMA
    • Organizer
      89th General Session and Exhibition of IADR/AADR/CADR
    • Place of Presentation
      San Diego,国際会議場CA USA
    • Related Report
      2010 Annual Research Report
  • [Presentation] ヒト歯根膜細胞のメカニカルストレスによる遺伝子発現2010

    • Author(s)
      荒川 俊哉、岡山 三紀、溝口 到、設楽 彰子、田隈 泰信
    • Organizer
      第83回日本生化学会
    • Place of Presentation
      神戸市
    • Related Report
      2012 Final Research Report
  • [Presentation] 開口分泌のSNAREタンパク質は過剰に存在するか?2010

    • Author(s)
      田隈 泰信、岡山 三紀、設楽 彰子、荒川俊哉、溝口 到
    • Organizer
      第83回日本生化学会
    • Place of Presentation
      神戸市.
    • Related Report
      2012 Final Research Report
  • [Presentation] 点特異的メチル基導入ヘクターのES細胞での発現調節2010

    • Author(s)
      荒川俊哉、安彦善裕、岡山三紀、溝口到、設楽彰子、田隈泰信
    • Organizer
      第52回歯科基礎医学会学術大会
    • Place of Presentation
      東京都船堀会議場
    • Related Report
      2010 Annual Research Report
  • [Presentation] ヒト歯根膜細胞のメカニカルストレスによる遺伝子発現2010

    • Author(s)
      荒川俊哉、岡山三紀、溝口到、設楽彰子、田隈泰信
    • Organizer
      第83回日本生化学会大会
    • Place of Presentation
      神戸ポートアイランド会議場
    • Related Report
      2010 Annual Research Report
  • [Presentation] ヒト歯根膜におけるリゾホスファチジン酸シグナル

    • Author(s)
      荒川俊哉、岡山三紀、小原伸子、設楽彰子、入江一元、溝口到、田隈泰信
    • Organizer
      日本歯科基礎医学会
    • Place of Presentation
      奥羽大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] ヒト歯根膜におけるリゾホスファチジン酸の合成およびシグナル経路の解析

    • Author(s)
      荒川俊哉、岡山三紀、設楽彰子、溝口到、田隈泰信
    • Organizer
      日本生化学会
    • Place of Presentation
      福岡国際会議場
    • Related Report
      2012 Annual Research Report
  • [Presentation] Crosstalk Between Lysophosphatidic Acid and Mechanical Stress in Periodontal Ligament

    • Author(s)
      Toshiya Arakawa, Miki Okayama, Akiko Shitara, Itaru Mizoguchi, Taishin Takuma
    • Organizer
      IADR
    • Place of Presentation
      Seattle convention center
    • Related Report
      2012 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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