Establishment of an animal-free systems for human dental pulp-derived stem cell culture and induction of iPS cells
| Project/Area Number |
22592181
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Dental engineering/Regenerative dentistry
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| Research Institution | Gifu University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
SHIBATA Toshiyuki 岐阜大学, 医学(系)研究科, 教授 (50226172)
MAKITA Hiroki 岐阜大学, 医学(系)研究科, 講師 (50345790)
HATAKEYAMA Daijiro 岐阜大学, 医学(系)研究科, 助教 (60377653)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 再生歯学 / 歯学 / 組織幹細胞 / 歯髄 |
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| Research Abstract |
Human dental pulp stem cell (DPSC) lines are usually established in the medium containing fetal bovine serum (FBS). FBS is an important additive for cell growth, however, the allergenic potential and the possibility of contamination can not be excluded. In this study, we established DPSCs with two kinds of medium: 1) medium containing FBS or 2) serum-free medium. DPSCs grown under the condition containing FBS, showed significantly higher primary colony formation than those under serum-free condition. But once established, serum-free condition was applicable for culture, preservation and iPS cells induction.
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Report
(4 results)
Research Products
(18 results)
