Development of a highly sensitive genetic diagnosis ofSjogren syndrome based on detection ofβdefensins

• Project/Area Number: 22592215
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Okayama University
• Principal Investigator: MIZUKAWA Nobuyoshi 岡山大学, 岡山大学病院, 講師 (00263608)
• Co-Investigator(Kenkyū-buntansha): YAMAAI Yuichiro 岡山大学, 大学院・医歯薬学総合研究科, 助教 (00158057) ; TAKAGI Shin 岡山大学, 大学院・医歯薬学総合研究科, 准教授 (40116471) ; NAGATSUKA Hitoshi 岡山大学, 大学医歯薬学総合研究科, 教授 (70237535)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2010: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: ヒトβデフェンシン / シェーグレン症候群 / リアルタイムRT-PCR / ヒトベータデフェンシン / RT-PCR / 遺伝子発現 / 迅速診断法 / シェーングレン症候群 / βデフェンシン

Research Abstract

To analyze the details of changes in expression patterns of human β-defensins in labial glands in Sjogren’s syndrome, the present study investigated the immunoreactivity and gene expression of human β-defensin s in patients with and without Sjogren’s syndrome. Otherwise, we analyzed the localization patterns of β defensins , in the tracheal epithelium of mouse modeles of sick building mice. The results of this study indicated that acute inflammation increased β defensin4 and chronic inflammation decreased β defensin4. Weak gene expression of human β-defensin4 was detected in labial glands with Sjogren’s syndrome, but was almost absent in labial glands without Sjogren’s syndrome. Interestingly, a subgroup of labial glands without Sjogren’s syndrome showed gene expression of human β-defensin4and similar morphology to the Sjogren’s syndrome. These findings and the result that acute inflammation increased β defensin4 and chronic inflammation decreased β defensin4 may indicate a potential for future Sjogren’s syndrome. These analyses might prove useful in the diagnosing and assessing risk of Sjogren’s syndrome.

Research Products

• [Journal Article] Toluene exposure leads to a change in expression patterans of β defensins in the mouse tracheal epithelium (2013)
  • Author(s): Seiko Takeda, Yuichiro Yamaai, Yoshihiro Kaneda, Nobuyoshi Mizukawa, Seiji Iida, and Hidekazu Fujimaki
  • Journal Title: J Toxicol Pathol Volume: 26 Pages: 35-40
  • Peer Reviewed
• [Journal Article] Toluene Exposure Leads to a Change in Expression Patterns of β Defensins in the Mouse Tracheal Epithelium (2013)
  • Author(s): 武田斉子
  • Journal Title: Journal of Toxicologic Pathology Volume: 26 Issue: 1 Pages: 35-40
  • DOI: 10.1293/tox.26.35
  • NAID: 130003362356
  • ISSN: 0914-9198, 1881-915X
  • Peer Reviewed
• [Presentation] シェーグレン症候群患者の口唇腺におけるヒトβデフェンシンの局在と臨床応用 (2011)
  • Author(s): 金田祥弘・水川展吉
  • Organizer: 第56回日本口腔外科学会総会・学術大会
  • Place of Presentation: 大阪国際会議場
  • Year and Date: 2011-10-21
• [Presentation] デフェンシン遺伝子を標的にしたRT-PCR法によるシェーグレン症候群の迅速診断 (2011)
  • Author(s): 武田斉子・水川展吉
  • Organizer: 第56回日本口腔外科学会総会・学術大会
  • Place of Presentation: 大阪国際会議場
  • Year and Date: 2011-10-21
• [Presentation] Change in expression pattern of human β defensins in mouse tracheal epithelium induced by toluene exposure (2010)
  • Author(s): 武田斉子
  • Organizer: 第17回 日本免疫毒性学会学術大会
  • Place of Presentation: 独立行政法人大山記念ホール
  • Year and Date: 2010-09-10
• [Presentation] Change in expression pattern of human β defensins in mouse tracheal epithelium induced by toluene exposure. (2010)
  • Author(s): 武田斉子(研究協力者)
  • Organizer: 第17回 日本免疫毒性学会学術大会
  • Place of Presentation: 独立行政法人国立環境研究所 大山記念ホール
  • Year and Date: 2010-09-10