| Project/Area Number |
22592215
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Okayama University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
YAMAAI Yuichiro 岡山大学, 大学院・医歯薬学総合研究科, 助教 (00158057)
TAKAGI Shin 岡山大学, 大学院・医歯薬学総合研究科, 准教授 (40116471)
NAGATSUKA Hitoshi 岡山大学, 大学医歯薬学総合研究科, 教授 (70237535)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2010: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | ヒトβデフェンシン / シェーグレン症候群 / リアルタイムRT-PCR / ヒトベータデフェンシン / RT-PCR / 遺伝子発現 / 迅速診断法 / シェーングレン症候群 / βデフェンシン |
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| Research Abstract |
To analyze the details of changes in expression patterns of human β-defensins in labial glands in Sjogren’s syndrome, the present study investigated the immunoreactivity and gene expression of human β-defensin s in patients with and without Sjogren’s syndrome. Otherwise, we analyzed the localization patterns of β defensins , in the tracheal epithelium of mouse modeles of sick building mice. The results of this study indicated that acute inflammation increased β defensin4 and chronic inflammation decreased β defensin4. Weak gene expression of human β-defensin4 was detected in labial glands with Sjogren’s syndrome, but was almost absent in labial glands without Sjogren’s syndrome. Interestingly, a subgroup of labial glands without Sjogren’s syndrome showed gene expression of human β-defensin4and similar morphology to the Sjogren’s syndrome. These findings and the result that acute inflammation increased β defensin4 and chronic inflammation decreased β defensin4 may indicate a potential for future Sjogren’s syndrome. These analyses might prove useful in the diagnosing and assessing risk of Sjogren’s syndrome.
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